Toxicology International (Formerly Indian Journal of Toxicology)

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Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice


Affiliations
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, India
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, India
     

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Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.

Keywords

AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.
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  • Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice

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Authors

Rashmi Singh
Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, India
Veena Sharma
Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, India

Abstract


Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.

Keywords


AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.

References





DOI: https://doi.org/10.18311/ti%2F2019%2Fv26i1%2F23997