Toxicology International (Formerly Indian Journal of Toxicology)

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Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice


Affiliations
1 Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, India
2 Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, India
     

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Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.

Keywords

AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.
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  • Abdallah H, Mohamed M, Abdou A, Hamed M, AbdelNaim A, Ashour O. Protective effect of Centaurea pallescens Del. against CCl4-induced injury on a human hepatoma cell line (Huh7). Med Chem Res. 2013; 22:5700–6. https:// doi.org/10.1007/s00044-013-0563-y

  • Srivastava A, Shivanandappa T. Hepatoprotective effect of the ischolar_main extract of Decalepishamiltonii against carbon tetrachlorideinduced oxidative stress in rats. Food Chem. 2010; 118:411–7. https://doi.org/10.1016/j.foodchem.2009.05.014

  • Kerry JP, Kerry JF. Processed meats: Improving safety, nutrition and quality. Sinko P, editor. Heat and processing generated contaminants in processed meats. Cambridge: Woodhead Publishing Ltd. 2011. https://doi.org/10.1533/9780857092946

  • Hecht SS, Upadhyaya P, Wang M. Evolution of research on the DNA Adduct Chemistry of N-Nitrosopyrrolidine and Related Aldehydes. Chem ResToxicol. 2011; 24:781–90. PMid: 21480629 PMCid: PMC3118975. https://doi.org/10.1021/tx200064a

  • Nadkarni KM.I ndian Materia medica. Bombay: Popular Prakashan; 1996.

  • Savithramma N, Sulochana Ch, Rao KN. Ethnobotanical survey of plants used to treat asthma in Andhra Pradesh, India. J Ethnopharmacol. 2007; 113:4–61. PMid: 17606346. https://doi.org/10.1016/j.jep.2007.04.004

  • Motamarri SN, Arthikeyan M, Rajsekar S, Gopal V. Indigofera tinctoria Linn - A phytopharmacological review. Int J Res Pharm BiomedSci. 2012; 3:164–9.

  • Kris-Etherton PM, Hecker HD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer. Am J Med. 2002; 113:71S–88S. https://doi.org/10.1016/S0002-9343(01)00995-0

  • Khandelwal KP. Practical Pharmacology: Techniques and experiments. Pune: Nirali Prakashan; 2006.

  • Sharma V, Singh R. Isolation and structural elucidation of an isothiocyanate compound from Indigoferatinctoria Linn. extract. Curr Sci. 2017; 113:941–6. https://doi.org/10.18520/cs/v113/i05/941-946

  • Singh BN, Singh BR, Sharma BK, Singh HB. Potential chemoprevention of N-nitrosodiethylamine-induced hepatocarcinogenesis by polyphenolics from Acacia nilotica bark. Chem Biol Interact. 2009; 181:20–8. PMid: 19446540. https://doi.org/10.1016/j.cbi.2009.05.007

  • Priyadarsini G, Kumar A, Anbu J, Anjana A, Ayyaswamy A. Nephroprotective activity of decoction of Indigofera tinctoria (Avurikudineer) against cisplatin-induced nephropathy in rats. Int J Life Sci Pharm Res. 2012; 2:56–62.

  • Tian CC, Zha XQ, Luo JP. A polysaccharide from Dendrobium huoshanensehepatic inflammatory response caused by carbon tetra chloride. Biotechnol Biotechnol Equip 2015; 29:132–8. PMid: 26019626 PMCid: PMC4434038. https://doi.org/10.1080/13102818.2014.987514

  • Reitman S, Frankel S. A method of assaying liver enzymes in human serum. Am J Clin Pathol. 1957; 28:56–8. PMid: 13458125. https://doi.org/10.1093/ajcp/28.1.56

  • Sadashivam S, Manickam A. Phenolics: Biochemical Methods. New Delhi: New Age International Publishers; 2004.

  • Floyd RA, Soong LM, Stuart MA, Reigh DL. Spin trapping of free radicals produced from nitrosamine carcinogens. Photochem Photobiol. 1978; 28:857–62. PMid: 216035. https://doi.org/10.1111/j.1751-1097.1978.tb07032.x

  • Rao GM, Rao Ch.V, Pushpangadan P, Annie S. Hepatoprotective effects of rubiadin, a major constituent of Rubia Cordifolia Linn. J Ethnopharmacol. 2006; 103:484–90. PMid: 16213120. https://doi.org/10.1016/j.jep.2005.08.073

  • Rej R. Measurement of aminotransferases: Part 1. Aspartate aminotransferases. CRC Crit Rev Clin Lab Sci. 1984; 21:99–106. PMid: 6391817. https://doi.org/10.3109/10408368409167137

  • Dufour DR, Lott JA, Nolte FS, Gretch DR, Koff RS, Seeff LB. Diagnosis and monitoring of Hepatic injury: Recommendations for use of Laboratory tests in screening, diagnosis and monitoring. Clin Chem. 2000; 46:2050–68.

  • Pol S, Nalpas B, Vassault A, Bousquet-Lemercier B, Franco D, Lacour B, et al. Hepatic activity and mRNA expression of aspartate aminotransferase isoenzymes in alcoholic and non-alcoholic liver disease. Hepatology 1991; 14:620–5. https://doi.org/10.1002/hep.1840140408

  • Jahan R, Vani G, Shyamaladevi CS. Anticarcinogenic effect of Solanum trilobatum in Diethylnitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rats. Asian J Biochem. 2011; 6:74–81. https://doi.org/10.3923/ajb.2011.74.81

  • Sahu CR. Mechanisms involved in toxicity of liver caused by Piroxicam in mice and protective effects of leaf extracts of Hibiscus rosa-sinensis L. Clin Med Insights Artheritis Musculoskelet Disord. 2016; 9:9–13. PMid: 26819562 PMCid: PMC4720181. https://doi.org/10.4137/CMAMD.S29463

  • Singh R, Kumar S, Rana AC, Sharma N. Different models of Hepatotoxicity and related liver disease: A review. Int Res J Pharma. 2012; 3:86–95.

  • MLAB2401- Clinical Chemistry Lab Manual CF113. UNIT: Total and Direct Bilirubin. www.2ndchance.info/dxme-BilirubinUrine-defDirect.pdf

  • Singh R, Sharma S, Sharma V. Comparative and quantitative analysis of antioxidant and scavenging potential of Indigofera tinctoria Linn. extracts. J Integr Med. 2015; 13:269–78. https://doi.org/10.1016/S2095-4964(15)60183-2


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  • Anti-hepatotoxic Potential of Indigofera tinctoria and its isolated Isothiocyanate compound ‘ITC-1’ against NPYR-CCl4 Intoxicated Mice

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Authors

Rashmi Singh
Department of Biotechnology and Microbiology, Alpine Group of Management and Technology, Dehradun – 248001, Uttarakhand, India
Veena Sharma
Department of Bioscience and Biotechnology, Banasthali University, Vanasthali – 304022, Rajasthan, India

Abstract


Isothiocyanate derivative from hydroethanolic extract of Indigofera tinctoria (HEIT) was previously isolated, purified and characterized as 1-[1,2-Diisothiocyanato-2-(3-isothiocyanato-2,2-dimethyl-propylsulfanyl)-ethoxy]-3-isothiocyanato-2,2-dimethyl-propane (C16H22N4OS5; m/z 446.70; ITC-1). To elucidate hepatoprotective efficacy, liver toxicity was induced in mice via intoxication of N-Nitrosopyrrolidine (NPYR) followed by CCl4 for 50 days. Both low and high doses of crude HEIT were given orally to NPYR treated mice for 21 days. Silymarin was also administered to compare the results. After completion of post treatment, various hepatic toxicity markers were evaluated. Results showed that both doses of HEIT and ITC-1 have successfully normalized the levels of AST, ALT (P<0.001 v/s NPYR treated group), ALP and bilirubin (P<0.01). ITC-1 has showed better remedial response against liver toxicity in comparison to Silymarin. Thus, we concluded that both I. tinctoria and ‘ITC-1’ have future remedial aspect in diminution of hepatic toxicity.

Keywords


AST, Bilirubin, Hepatic Toxicity, Indigofera tinctoria, Isothiocyanate Compounds, Silymarin.

References





DOI: https://doi.org/10.18311/ti%2F2019%2Fv26i1%2F23997