Toxicology International (Formerly Indian Journal of Toxicology)

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Therapeutic Role of Mitochondria Targeted Curcumin against Pseudomonas aeruginosa Induced Oxidative Stress in Thyroid Gland of Female Swiss albino Mice


Affiliations
1 Endocrinology Laboratory, Department of Zoology, School of Biological Sciences, Dr Harisingh Gour University, Sagar – 470003, Madhya Pradesh, India
     

   Subscribe/Renew Journal


Bacteria are found universally and contaminate the soil, air and water. Through contaminated air, water and soil bacteria reach inside the body and causes immense harm. The problem of thyroid is that, it affects the public health worldwide. The main purpose of this investigation is to find out the toxic effects of Pseudomonas aeruginosa on thyroid gland and preventive role of mitochondria targeted curcumin in bacterial induced thyroid toxicity in female Swiss albino mice. The animals are separated in different groups. Group I served as control and Group II administered with effective dose of Pseudomonas aeruginosa. Group III co-treated with mitochondria targeted curcumin for the period of 14 days. With the completion of treatment period blood serum and tissue samples were collected for determining the level of thyroid hormones and oxidative stress. In this investigation shows that mice exposed to bacteria have decreased T3 and T4 concentration as compared to control and co-treated mice have some elevated level of T3 and T4 hormones as compared to bacteria treated mice. In the biochemical study it is found that the increased lactate dehydrogenate, catalase activity, superoxide dismutase and lipid peroxidation and decreased glutathione reductase points towards the induced oxidative stress in thyroid gland. It is concluded that the exposure to Pseudomonas aeruginosa causes thyroid problem and treatment with mitochondria targeted curcumin prevents the induced toxicity.

Keywords

Mitochondria Targeted Curcumin, Pseudomonas aeruginosa, Thyroid Gland, T3, T4, TSH.
User
Subscription Login to verify subscription
Notifications
Font Size

  • Frohlich E, Wahl R. Microbiota and thyroid interaction in health and disease. Trends in Endo and Meta. 2019; 30(8):479–90. PMid: 31257166. https://doi.org/10.1016/j.tem.2019.05.008

  • Ramos E, Ramirez-Bahena MH, Valverde A, Velazquer E, Zuniga D, Velezmoro C, Peix A. Pseudomonas punonensis sp. nov., isolated from straw. Int J of Syst and Evolut Microbio. 2013; 63:1834–9. PMid: 23002045. https://doi.org/10.1099/ijs.0.042119-0

  • Moore JE, Heaney N, Millar BC, Crowe M, Elborn JS. Incidence of Pseudomonas aeruginosa in recreational and hydrotherapy pools. Communicable Disease and Public Health. 2002; 5(1):23–6.

  • Fiorillo L, Zucker M, Sawyer D, Lin AN. The pseudomonas hot-foot syndrome. New England Journal of Medicine. 2001; 345(5):335–8. PMid: 11484690. https://doi.org/10.1056/NEJM200108023450504

  • Blanc DS, Nahimana I, Petignat C, Wenger A, Bille J, Francioli P. Faucets as a reservoir of endemic Pseudomonas aeruginosa colonization/infections in intensive care units. Int Care Med. 2004; 30(10):1964–8. PMid: 15257431. https://doi.org/10.1007/s00134-004-2389-z

  • Gerwing J, Long DA, Pitt-Rivers R. The influence of bacterial exotoxins on the activity of the thyroid gland in different species. The J of Physiol. 1958; 144(2):229– 42. PMid: 13611689 PMCid: PMC1356738. https://doi.org/10.1113/jphysiol.1958.sp006097

  • Lee SE, Shin HT, Hwang HJ, Kim JH. Antioxidant activity of extracts from Alpinia katsumadai seed: Phytotherapy research. An Int J Devoted to Pharmacol and Toxicol Eva of Natural Product Derivatives. 2003; 17(9):1041–7. PMid: 14595584. https://doi.org/10.1002/ptr.1291

  • Braekke K, Harsem NK, Staff AC. Oxidative stress and antioxidant status in fetal circulation in preeclampsia. Pedia Res. 2006; 60(5):560–4. PMid: 16988193. https://doi.org/10.1203/01.pdr.0000242299.01219.6a

  • Valko M, Rhodes C, Moncol J, Izakovic MM, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biolog Interact. 2006; 160(1):1–40. PMid: 16430879. https://doi.org/10.1016/j.cbi.2005.12.009

  • Praditya D, Kirchhoff L, Bruning J, Rachmawati H, Steinmann J, Steinmann E. Anti-infective properties of the golden spice curcumin. Frontiers in Microbial. 2019; 10:1–16. PMid: 31130924 PMCid: PMC6509173. https://doi.org/10.3389/fmicb.2019.00912

  • Cocheme HM, Murphy MP. Can antioxidants be effective therapeutics. Curr Opin Inves Drugs. 2010; 11(4):426–31.

  • Murphy MP, Smith RA. Targeting antioxidants to mitochondria by conjugation to lipophilic cations. Annu Rev Pharmacol Toxicol. 2007; 47:629–56. PMid: 17014364. https://doi.org/10.1146/annurev.pharmtox.47.120505.105110

  • Smith RA, Porteous CM, Coulter CV, Murphy MP. Selective targeting of an antioxidant to mitochondria. Europ J of Biochem. 1999; 263(3):709–16. PMid: 10469134. https://doi.org/10.1046/j.1432-1327.1999.00543.x

  • Kornberg A. Lactic dehydrogenase of muscle: Pyruvate+DPNH Lactate+ DPN. 1955; 441–3. https://doi.org/10.1016/0076-6879(55)01072-0

  • Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal Biochem. 1966; 16(2):359–64. https://doi.org/10.1016/0003-2697(66)90167-9

  • Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1):276–87. https://doi.org/10.1016/0003-2697(71)90370-8

  • Sachidhanandam M, Singh SN, Salhan AK, Ray US. Evaluation of plasma hormone concentrations using Enzyme-immunoassay/Enzyme-linked immunosorbent assay in healthy Indian men: Effect of ethnicity. Ind J of Cli Biochem. 2010; 25(2):153–7. PMid: 23105902 PMCid: PMC3453109. https://doi.org/10.1007/s12291-010-0028-8

  • Shewell J, Long DA. A species difference with regard to the effect of cortisone acetate on body weight, γ-globulin and circulating antitoxin levels. Epid and Inf. 1956; 54(4):452– 60. PMid: 13385481 PMCid: PMC2217895. https://doi.org/10.1017/S0022172400044739

  • Long DA. The influence of corticosteroids on immunological responses to bacterial infections. Inter Archives of Aller and Immun. 1957; 10(1):5–12. PMid: 13428355. https://doi.org/10.1159/000228360

  • Sakr SA, Saber A. Ameliorative effect of ginger (Zingiber officinale) on mancozeb fungicide induced liver injury in albino rats. Aust J Basic Appl Sci. 2007; 1(4):650–6.

  • Mezey E. Metabolic effects of alcohol. In Fed Proc. 1985; 44(1):134–8.

  • Malamos B, Koutras DA, Rigopoulos GA, Papapetrou PD, Gougas E, Kelperi H, Moraitopoulos C, Davi E, Leonardopoulos J. Endemic goiter in Greece: Some new epidemiologic studies. The J of Clin Endo and Met. 1971; 32(2):130–9. PMid: 4321500. https://doi.org/10.1210/jcem-32-2-130

  • Reid GM, Tervit H. Sudden infant death syndrome and placental disorders: The thyroid-selenium link. Med. Hypo. 1997; 48(4):317–24. https://doi.org/10.1016/S03069877(97)90100-6

  • Maekawa M, Inomata M, Sasaki MS, Kaneko A, Ushiama M, Sugano K, Takayama J, Kanno, T. Electrophoretic variant of a lactate dehydrogenase isoenzyme and selective promoter methylation of the LDHA gene in a human retinoblastoma cell line. Clin Chem. 2002; 48(11):1938–45. PMid: 12406979. https://doi.org/10.1093/clinchem/48.11.1938

  • Girotti AW, Thomas JP. Damaging effects of oxygen radicals on resealed erythrocyte ghosts. J of Bio Chem. 1984; 259(3):1744–52.

  • Balasubramanyam M, Koteswari AA, Kumar RS, Monickaraj SF, Maheswari JU, Mohan V. Curcumin-induced inhibition of cellular reactive oxygen species generation: Novel therapeutic implications. J of Biosci. 2003; 28(6):715–21. PMid: 14660871. https://doi.org/10.1007/BF02708432

  • Resch U, Helsel G, Tatzber F, Sinzinger H. Antioxidant status in thyroid dysfunction. Clin Chem and Lab Med. 2002; 40(11):1132–4. PMid: 12521231. https://doi.org/10.1515/cclm.2002.198

  • Guerrero A, Pamplona R, Portero-Otín M, Barja G, LopezTorres M. Effect of thyroid status on lipid composition and peroxidation in the mouse liver. Free Rad Bio and Med. 1999; 26(1-2):73–80. https://doi.org/10.1016/S08915849(98)00173-7

  • Yilmaz S, Ozan S, Benzer F, Canatan H. Oxidative damage and antioxidant enzyme activities in experimental hypothyroidism. Cell Biochem. and Function: Cell. Biochem. and its modulation by active agents or disease. 2003; 21(4):325–30. PMid: 14624470. https://doi.org/10.1002/cbf.1031

  • Vranova E, Inze D, Van Breusegem F. Signal transduction during oxidative stress. J of Exp Bot. 2002; 53(372):1227–36. PMid: 11997371. https://doi.org/10.1093/jexbot/53.372.1227

  • Kumar A, Mahobiya P. The efficiency of mitochondriatargeted curcumin in reducing thyroid mitochondrial oxidative damage. Inter J of Sci and Res. 2017; 6(1):1445– 50.


Abstract Views: 339

PDF Views: 0




  • Therapeutic Role of Mitochondria Targeted Curcumin against Pseudomonas aeruginosa Induced Oxidative Stress in Thyroid Gland of Female Swiss albino Mice

Abstract Views: 339  |  PDF Views: 0

Authors

Narendra Namdev
Endocrinology Laboratory, Department of Zoology, School of Biological Sciences, Dr Harisingh Gour University, Sagar – 470003, Madhya Pradesh, India
Arun Kumar
Endocrinology Laboratory, Department of Zoology, School of Biological Sciences, Dr Harisingh Gour University, Sagar – 470003, Madhya Pradesh, India
Gayatri Rai
Endocrinology Laboratory, Department of Zoology, School of Biological Sciences, Dr Harisingh Gour University, Sagar – 470003, Madhya Pradesh, India
Payal Mahobiya
Endocrinology Laboratory, Department of Zoology, School of Biological Sciences, Dr Harisingh Gour University, Sagar – 470003, Madhya Pradesh, India

Abstract


Bacteria are found universally and contaminate the soil, air and water. Through contaminated air, water and soil bacteria reach inside the body and causes immense harm. The problem of thyroid is that, it affects the public health worldwide. The main purpose of this investigation is to find out the toxic effects of Pseudomonas aeruginosa on thyroid gland and preventive role of mitochondria targeted curcumin in bacterial induced thyroid toxicity in female Swiss albino mice. The animals are separated in different groups. Group I served as control and Group II administered with effective dose of Pseudomonas aeruginosa. Group III co-treated with mitochondria targeted curcumin for the period of 14 days. With the completion of treatment period blood serum and tissue samples were collected for determining the level of thyroid hormones and oxidative stress. In this investigation shows that mice exposed to bacteria have decreased T3 and T4 concentration as compared to control and co-treated mice have some elevated level of T3 and T4 hormones as compared to bacteria treated mice. In the biochemical study it is found that the increased lactate dehydrogenate, catalase activity, superoxide dismutase and lipid peroxidation and decreased glutathione reductase points towards the induced oxidative stress in thyroid gland. It is concluded that the exposure to Pseudomonas aeruginosa causes thyroid problem and treatment with mitochondria targeted curcumin prevents the induced toxicity.

Keywords


Mitochondria Targeted Curcumin, Pseudomonas aeruginosa, Thyroid Gland, T3, T4, TSH.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i1%262%2F24457