Toxicology International (Formerly Indian Journal of Toxicology)

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Potential Protective Effect of Magnesium Supplementation on Nickel Induced Hepatotoxicity in Albino Wistar Rats


Affiliations
1 Department of Biochemistry, Faculty of Sciences, Applied Biochemistry and Microbiology Laboratory, University of Annaba, 23000 Annaba, Algeria
2 3Department of Biology, Faculty of Science, Ecophysiology Animal Laboratory, University of Sfax, 3038 Sfax., Tunisia
     

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Nickel is considered one of the most harmful heavy metals on human health. This experimental study was designed to investigate whether magnesium supplementation has any influence on nickel-induced toxicity in albino rats. Twenty one adult rats were divided into three groups of seven animals each group. The first group was used as a control and received saline solution. The second group was administered with nickel sulfate (2.0 mg/100 g, i.p) and the third group was given both nickel sulfate (2.0 mg/100 g, i.p.) and magnesium sulfate (300 mg/kg, i.p) simultaneously on alternate days. Body weight gain was recorded regularly. After 21 consecutive days, cellular functions were evaluated by biochemical and oxidative stress markers. The nickel sulfate-treatment decreased body weight gain in rats. Nickel treatment produced also oxidative injury characterized by an increase in glucose level, enzymes markers activities, lipid profile and total proteins and bilirubin concentrations. Simultaneously, glutathione level (GSH) and catalase activity was diminished in liver tissue. These results are further substantiated with obviously changes in hepato- histoarchithecture. However, the treatment with magnesium ameliorated the previous parameters and resulted in an improvement of the histopathological lesions. Our results suggest that

Keywords

Hepatic Toxicity, Histopathology, Magnesium, Nickel, Oxidative Stress, Rats
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  • Potential Protective Effect of Magnesium Supplementation on Nickel Induced Hepatotoxicity in Albino Wistar Rats

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Authors

Mohamed Khiari
Department of Biochemistry, Faculty of Sciences, Applied Biochemistry and Microbiology Laboratory, University of Annaba, 23000 Annaba, Algeria
Zine Kechrid
Department of Biochemistry, Faculty of Sciences, Applied Biochemistry and Microbiology Laboratory, University of Annaba, 23000 Annaba, Algeria
Fahima Klibet
Department of Biochemistry, Faculty of Sciences, Applied Biochemistry and Microbiology Laboratory, University of Annaba, 23000 Annaba, Algeria
Mongi Saoudi
3Department of Biology, Faculty of Science, Ecophysiology Animal Laboratory, University of Sfax, 3038 Sfax., Tunisia

Abstract


Nickel is considered one of the most harmful heavy metals on human health. This experimental study was designed to investigate whether magnesium supplementation has any influence on nickel-induced toxicity in albino rats. Twenty one adult rats were divided into three groups of seven animals each group. The first group was used as a control and received saline solution. The second group was administered with nickel sulfate (2.0 mg/100 g, i.p) and the third group was given both nickel sulfate (2.0 mg/100 g, i.p.) and magnesium sulfate (300 mg/kg, i.p) simultaneously on alternate days. Body weight gain was recorded regularly. After 21 consecutive days, cellular functions were evaluated by biochemical and oxidative stress markers. The nickel sulfate-treatment decreased body weight gain in rats. Nickel treatment produced also oxidative injury characterized by an increase in glucose level, enzymes markers activities, lipid profile and total proteins and bilirubin concentrations. Simultaneously, glutathione level (GSH) and catalase activity was diminished in liver tissue. These results are further substantiated with obviously changes in hepato- histoarchithecture. However, the treatment with magnesium ameliorated the previous parameters and resulted in an improvement of the histopathological lesions. Our results suggest that

Keywords


Hepatic Toxicity, Histopathology, Magnesium, Nickel, Oxidative Stress, Rats

References





DOI: https://doi.org/10.18311/ti%2F2021%2Fv28i2%2F26691