Toxicology International (Formerly Indian Journal of Toxicology)

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Evaluation of Analgesic, Antidiarrheal and Hypoglycemic Activities of Wendlandia paniculata (Roxb.) DC Leaves Extract using Mice Model


Affiliations
1 Department of Environmental Science and Technology, JUST, Jashore – 7408., Bangladesh
2 1Department of Chemistry, JUST, Jashore – 7408., Bangladesh
3 Department of Pharmacy, SUB, 77 Satmasjid road, Dhanmandi, Dhaka – 1205, Bangladesh
4 Department of Chemistry, DU, Dhaka – 1000.
     

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The leaves of Wendlandia paniculata (Roxb.) DC is a rare folk medicine among tribal people even though there is no scientific literature available regarding its pharmacological potentials. This study aimed to evaluate the effects of crude methanol extract of W. paniculata (Roxb.) DC. Leaves in mice model. The in vivo glucose-lowering capacity of methanolic crude extract was analyzed utilizing the well-known tail tipping method. The central and peripheral analgesic activity was evaluated using the ‘tail flick’ and writhing assay respectively. The anti-diarrheal activity was evaluated using the method of castor oil-induced diarrhea in mice. As a result, oral administration (400 mg/kg) of the extract resulted significant (P<0.001) delay in response to pain and inhibition of acetic acid-induced writhing response. The results were comparable to the respective standards morphine (2 mg/kg) and diclofenac (50 mg/kg). Likewise, maximum reduction (p<0.05) of blood glucose (32.3%) was observed 180 min after oral intake(400 mg/kg) of the extract compared to the 47% of the standard glibenclamide. Moreover, a reduction of diarrheal feces was observed 45.83% (p<0.001) compared to standard loperamide 70.83%. The in vivo bioassays confirmed that the crude methanol extract of leaves of W. paniculata possesses significant analgesic (central, peripheral) and antidiarrheal activity with mild glucose-lowering activity. Isolation of bioactive compounds is needed to justify the needed molecule responsible for the activity.


Keywords

Analgesic, Antidiarrheal, Hypoglycemic, Wendlandia paniculata
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  • Evaluation of Analgesic, Antidiarrheal and Hypoglycemic Activities of Wendlandia paniculata (Roxb.) DC Leaves Extract using Mice Model

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Authors

Md. Sabbir Hossain
Department of Environmental Science and Technology, JUST, Jashore – 7408., Bangladesh
Mohammad Mahmudul Hasan
1Department of Chemistry, JUST, Jashore – 7408., Bangladesh
Mohammad Abdullah Taher
Department of Pharmacy, SUB, 77 Satmasjid road, Dhanmandi, Dhaka – 1205, Bangladesh
Tauhidur Rahman
Department of Chemistry, DU, Dhaka – 1000.

Abstract


The leaves of Wendlandia paniculata (Roxb.) DC is a rare folk medicine among tribal people even though there is no scientific literature available regarding its pharmacological potentials. This study aimed to evaluate the effects of crude methanol extract of W. paniculata (Roxb.) DC. Leaves in mice model. The in vivo glucose-lowering capacity of methanolic crude extract was analyzed utilizing the well-known tail tipping method. The central and peripheral analgesic activity was evaluated using the ‘tail flick’ and writhing assay respectively. The anti-diarrheal activity was evaluated using the method of castor oil-induced diarrhea in mice. As a result, oral administration (400 mg/kg) of the extract resulted significant (P<0.001) delay in response to pain and inhibition of acetic acid-induced writhing response. The results were comparable to the respective standards morphine (2 mg/kg) and diclofenac (50 mg/kg). Likewise, maximum reduction (p<0.05) of blood glucose (32.3%) was observed 180 min after oral intake(400 mg/kg) of the extract compared to the 47% of the standard glibenclamide. Moreover, a reduction of diarrheal feces was observed 45.83% (p<0.001) compared to standard loperamide 70.83%. The in vivo bioassays confirmed that the crude methanol extract of leaves of W. paniculata possesses significant analgesic (central, peripheral) and antidiarrheal activity with mild glucose-lowering activity. Isolation of bioactive compounds is needed to justify the needed molecule responsible for the activity.


Keywords


Analgesic, Antidiarrheal, Hypoglycemic, Wendlandia paniculata

References





DOI: https://doi.org/10.18311/ti%2F2021%2Fv28i2%2F26775