Toxicology International (Formerly Indian Journal of Toxicology)

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Acute, Sub-Acute and Genotoxicity Assessment of Cocculus hirsutus Hydroalcoholic Leaf Extract in Wistar Rats


Affiliations
1 College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh, India
2 College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh., India
3 2Vignan Institute of Pharmaceutical Technology, Duvvada, Visakhapatnam – 534202, Andhra Pradesh, India
     

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Acute (0, 500, 1000 and 2000 mg/kg) and 28-day (0, 100, 200 and 400 mg/kg) toxicity assessments of Csocculu  hirsutus hydroalcoholic leaf extract (CH-Ex) were performed in Wistar rats consistent with OECD guidelines 423 and 407 respectively. Average body weights were recorded, blood and biochemical parameters were analyzed and histopathological studies were performed. At doses (12.5–100 μg/ml), genotoxicity assessments were conducted on pUC 19 plasmid by evaluating DNA damage protection. Acute toxicity assessment categorized CH-Ex as safe and non-toxic (category 5). The 28-day toxicity assessments showed no significant (p>0.05) changes in body weights, hematology and histopathology. At 400 mg/kg females exhibited a slight decline in SGPT, creatinine and BUN levels, while male rats exhibited a slight enhancement of SGOT and ALP levels (p<0.05). CH-Ex exhibited strong ability to protect DNA damage from oxidative stress in a dose-dependent manner.

Keywords

Biochemical, Cocculus hirsutus, DNA Damage, Histopathological, Oxidative Stress, Toxicity
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  • Acute, Sub-Acute and Genotoxicity Assessment of Cocculus hirsutus Hydroalcoholic Leaf Extract in Wistar Rats

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Authors

Uma Sankar Gorla
College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh, India
G. S. N. Koteswara Rao
College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh, India
Umasankar Kulandaivelu
College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh, India
Rajasekhar Reddy Alavala
College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh., India
Siva Prasad Panda
College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram – 522502, Andhra Pradesh., India
Rajkiran Kolakota
2Vignan Institute of Pharmaceutical Technology, Duvvada, Visakhapatnam – 534202, Andhra Pradesh, India

Abstract


Acute (0, 500, 1000 and 2000 mg/kg) and 28-day (0, 100, 200 and 400 mg/kg) toxicity assessments of Csocculu  hirsutus hydroalcoholic leaf extract (CH-Ex) were performed in Wistar rats consistent with OECD guidelines 423 and 407 respectively. Average body weights were recorded, blood and biochemical parameters were analyzed and histopathological studies were performed. At doses (12.5–100 μg/ml), genotoxicity assessments were conducted on pUC 19 plasmid by evaluating DNA damage protection. Acute toxicity assessment categorized CH-Ex as safe and non-toxic (category 5). The 28-day toxicity assessments showed no significant (p>0.05) changes in body weights, hematology and histopathology. At 400 mg/kg females exhibited a slight decline in SGPT, creatinine and BUN levels, while male rats exhibited a slight enhancement of SGOT and ALP levels (p<0.05). CH-Ex exhibited strong ability to protect DNA damage from oxidative stress in a dose-dependent manner.

Keywords


Biochemical, Cocculus hirsutus, DNA Damage, Histopathological, Oxidative Stress, Toxicity

References





DOI: https://doi.org/10.18311/ti%2F2021%2Fv28i2%2F26814