Open Access
Subscription Access
Open Access
Subscription Access
Evaluation of Haematological and Biochemical Activity of Thai Traditional Antinausea Remedy in Normal Albino Rats
Subscribe/Renew Journal
The present study was aimed to evaluate the effect of Thai Traditional anti-nausea remedy against haematological and biochemical effects in animals in the acute toxicity and sub-acute toxicity phase. Twenty-two male and female rats were divided into four groups with equal numbers of sex, each group were respectively received distilled water, 500, 1000 and 2000 mg/kg body weight as a single dose by gavage. While the experiment in the sub-acute phase, eighteen male and female rats were divided into three groups with equal numbers of sex. Each group were respectively given distilled water, 500 and 1000 mg/kg body weight once a day for the duration of the experiment until collecting blood samples. The blood samples were collected for haematological: Red Blood Cell (RBC), Haemoglobin (HGB), Hematocrit (HCT), Mean Corpuscular Volume (MCV), White Blood Cell (WBC), Neutrophil (NEU), Lymphocyte (LYMPH), Monocyte (MONO), Eosinophil (EO), Basophil (BASO) and biochemical analysis; Glucose (GLU), Blood Urea Nitrogen (BUN), Creatinine (CREA), Cholesterol (CHOL) and Triglyceride (TG). The results showed that the remedy caused behavioural adverse effects, but no mortality observed. In the acute toxicity phase, single oral administration of anti-nausea remedy increased mean difference compared to the control group on Creatinine level and decreased in AST level and ALP level (P<0.05). In the subacute study, daily oral administration of the remedy did not result in death or changes in the body weight and food consumption but also showed comparable results on renal function marker including increasing of BUN levels and decreasing of AST level and ALP level (P<0.05). In haematological and some biochemical analyses showed few disadvantageous effects. Based on the results obtained, although there were some changes in blood chemistry values, these were small changes not higher than reference values, indicating that the remedy was safe to take for a short period of time.
Keywords
Biochemical Activity, Haematological Activity, Kaelomvingvian Remedy, Thai Traditional Antinausea Remedy
User
Subscription
Login to verify subscription
Font Size
Information
- Saber Tehrani AS, Coughlan D, Hsieh YH, Mantokoudis G, Korley FK, Kerber KA, Frick KD, Newman-Toker DE, Schneider S. Rising annual costs of dizziness presentations to U.S. Emergency Departments. Academic Emergency Medicine. 2013; 20(7):689–96. PMid:23859582. https://doi. org/10.1111/acem.12168
- Thai Traditional and Alternative Health Profile: Thai Traditional Medicine, Indigenous Medicine and Alternative Medicine 2014-2016. Nonthaburi: Department of Thai Traditional and Alternative Medicine, Ministry of Public Health; 2017.
- National Drug System Development Committee. Thailand National List of Essential Medicines (NLEM). Government gazette. 2018; 135(14):228–31.
- Damjuti W, Kwansang J, Boonruab J. Effect of Thai traditional anti-nausea remedy on hypnotic and sedative activity in animal experimental models: Interaction with drugs acting at GABAA receptor. J Adv Pharm Technol Res. 2019; 10(2):85–9. PMid:31041188 PMCid:PMC6474159. https://doi. org/10.4103/japtr.JAPTR_361_18
- Delwatta SL, Gunatilake M, Baumans V, Seneviratne MD, Dissanayaka MLB., Batagoda SS, Udagedara AH,Walpola PB. Reference values for selected hematological, biochemical and physiological parameters of Sprague-Dawley rats at the Animal House, Faculty of Medicine, University of Colombo, Sri Lanka. Anim Models Exp Med. 2018; 1(4):250–4. PMid:30891574 PMCid:PMC6388088. https://doi. org/10.1002/ame2.12041
- Organisation for Economic Cooperation and Development (OECD). Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure. 2002.
- Organisation for Economic Cooperation and Development (OECD). Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure. 2008.
- Chimata M, Masaoka H, Fujimaki M, Hamada N, Oda K, Sudo M, Nagase M. Low serum aminotransferase activity in patients undergoing regular hemodialysis. Nihon Jinzo Gakkai Shi. 1994; 36(4):389–95.
- Ono K, Ono T, Matsumata T. The pathogenesis of decreased aspartate aminotransferase and alanine aminotransferase activity in the plasma of hemodialysis patients: The role of vitamin B6 deficiency. Clin Nephrol. 1995; 43(6):405–8.
- Siddique A, Kowdley KV. Approach to a patient with elevated serum alkaline phosphatase. Clin Liver Dis. 2012; 16(2):199–229. PMid:22541695 PMCid:PMC3341633. https://doi.org/10.1016/j.cld.20 12.03.012
- Nazari S, Rameshrad M, Hosseinzadeh H. Toxicological effects of Glycyrrhiza glabra (Licorice): A review. Phytother Res. 2017; 31(11):1635–50. PMid:28833680. https://doi.org/10.1002/ptr.5893
- Ehrenpreis JE, DesLauriers C, Lank P, Armstrong PK, Leikin JB. Nutmeg poisonings: A retrospective review of 10 years experience from the Illinois Poison Center, 2001-2011. J Med Toxicol. 2014; 10(2):148– 51. PMid:24452991 PMCid:PMC4057546. https://doi. org/10.1007/s13181-013-0379-7
- Li J, Yu Y, Yang Y, Liu X, Zhang J, Li B, Zhou X, Niu J, Wei X, Liu Z. A 15-day oral dose toxicity study of aspirin eugenol ester in Wistar rats. Food Chem Toxicol. 2012; 50(6):1980–5. PMid:22516304. https:// doi.org/10.1016/j.fct.2012.03.080
- Zhang H, Han T, Yu C-H, Jiang Y-P, Peng C, Ran X, Qin L-P. Analysis of the chemical composition, acute toxicity and skin sensitivity of essential oil from rhizomes of Ligusticum chuanxiong. J Ethnopharmacol. 2012; 144(3):791–6. PMid:23085396. https://doi. org/10.1016/j.jep.2012.10.010
- Kunanusorn P, Panthong A, Pittayanurak P, Wanauppathamkul S, Nathasaen N, Reutrakul V. Acute and subchronic oral toxicity studies of Nelumbo nucifera stamens extract in rats. J Ethnopharmacol. 2011; 134(3):789–95. PMid:21291992. https://doi. org/10.1016/j.jep.2011.01.037
Abstract Views: 88
PDF Views: 0