Toxicology International (Formerly Indian Journal of Toxicology)

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Neuroprotective Effect of Naringin on Acrylamideinduced Cytotoxicity in U87MG Cells and Wistar Rats: An in-vitro and in-vivo Study


Affiliations
1 Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati – 517502, Andhra Pradesh., India
2 Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 522019, Andhra Pradesh., India
     

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Acrylamide (ACR) is a potent neurotoxic to humans and animals. Neuroprotective effect of naringin was evaluated on ACR induced cytotoxicity using U87MG cells as in-vitro model and rat as in vivo model. ACR (50 mg/kg, i.p.) and Naringin (50 and 100 mg/kg) were administered to rats for 4 weeks. After 4 weeks, rats were sacrificed and sciatic nerves were isolated to determine the biochemical and apoptotic parameters. The exposure of U87MG cells to ACR reduced cell viability. Pretreatment of cells with 5–300 μg/ml naringin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Naringin down-regulated the Bax and upregulated the Bcl2 protein expression levels and also scavenged ACR induced free radicals in rats. In conclusion, our results indicated that pretreatment with naringin protected cells and rats from ACR-induced cytotoxicity and the neuroprotective effect was due to its antiapoptotic and antioxidant potential.

Keywords

Acrylamide, Apoptosis, Naringin, Neurotoxicity, Oxidative Stress, U87MG Cells.
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  • Neuroprotective Effect of Naringin on Acrylamideinduced Cytotoxicity in U87MG Cells and Wistar Rats: An in-vitro and in-vivo Study

Abstract Views: 180  |  PDF Views: 1

Authors

Thakur Santhrani.
Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati – 517502, Andhra Pradesh., India
Sathuluri Vineela.
Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 522019, Andhra Pradesh., India
Chinta Manga Devi.
Division of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Visvavidyalayam, Tirupati – 517502, Andhra Pradesh., India

Abstract


Acrylamide (ACR) is a potent neurotoxic to humans and animals. Neuroprotective effect of naringin was evaluated on ACR induced cytotoxicity using U87MG cells as in-vitro model and rat as in vivo model. ACR (50 mg/kg, i.p.) and Naringin (50 and 100 mg/kg) were administered to rats for 4 weeks. After 4 weeks, rats were sacrificed and sciatic nerves were isolated to determine the biochemical and apoptotic parameters. The exposure of U87MG cells to ACR reduced cell viability. Pretreatment of cells with 5–300 μg/ml naringin before ACR treatment significantly attenuated ACR cytotoxicity in a dose-dependent manner. Naringin down-regulated the Bax and upregulated the Bcl2 protein expression levels and also scavenged ACR induced free radicals in rats. In conclusion, our results indicated that pretreatment with naringin protected cells and rats from ACR-induced cytotoxicity and the neuroprotective effect was due to its antiapoptotic and antioxidant potential.

Keywords


Acrylamide, Apoptosis, Naringin, Neurotoxicity, Oxidative Stress, U87MG Cells.

References





DOI: https://doi.org/10.18311/10.18311%2Fti%2F2021%2Fv28i1%2F26267