Toxicology International (Formerly Indian Journal of Toxicology)

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid from Stem-bark of Prosopis Cineraria on Lps-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice


Affiliations
1 Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan, India
     

   Subscribe/Renew Journal


The present research aimed to elucidate the structure and characterize the isolated compound from stem-bark of Prosopis cineraria and unravel its potential against LPS-induced toxicity in mouse model. The spectral techniques were done for characterization and structure elucidation of the isolated compound (HPLC, NMR, FT-IR, LC-MS. The experimental mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with isolated compound from Prosopis cineraria (15 mg/kg body weight). Dexamethasone was used as a standard (10 mg/kg body weight). The oxidative stress parameters (LPO, CAT, SOD, GSH, GST and GPx) and biochemical activities (AST, ALT, ACP and ALP) were studied. The levels of pro-inflammatory cytokines (TNF-α; Prostaglandins E2; IL-6; NF-κBp65; IFN-γ and IL-10) were determined in liver homogenate. Nitric Oxide (NO) produced due to LPS-intoxication was determined by using Griess reagent. The results of the spectral analysis were used to elucidate the structure of the isolated flavonoid. The isolated flavonoid suppressed the over-expression and altered levels of oxidative parameters and cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- , Prostaglandin E2 and IL-10. The research investigation unfolded the alleviating potential of the isolated compound against LPS-induced adverse effects by modulating the expression of cytokines and combating oxidative stress.

Keywords

Anti-inflammatory, Cytokines, Flavonoids, Oxidative Stress, Prosopis cineraria, ROS
User
Subscription Login to verify subscription
Notifications
Font Size

  • Singer M, Deutschman CS, Seymour CW. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016; 315(8):801–10. PMid:26903338PMCid:PMC4968574. https://doi. org/10. 1001/jama.2016.0287

  • Callery MP, Kamei T, Flye MW. Kupffer cell blockade increases mortality during intra-abdominal sepsis despite improving systemic immunity. Arch Surg. 1990;125(1):36–41.PMid:2294881. https://doi. org/10.1001/archsurg.1990.01410130038005

  • Nostro A, Germano MP, D’angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett.o Appl.o Microbiol. 2000; 30:379. PMid: 10792667. https://doi.org/10.1046/j.1472-765x.2000.00731.x

  • Nandkarni KM. Indian Materia Medica. Vol. 1. Popular Prakashan: Mumbai; 2000.

  • Mohammad IS, Muhammad H, Shoaib Khan NA, Rasool F. Biological potential and phytochemical evaluation of Prosopis cineraria. World Appl Sci J. 2013; 27:1489–94.

  • Sumathi S, Dharani B, Sivaprabha J. Cell death induced by methanolic extract of Prosopis cineraria leaves in MCF-7 breast cancer cell line. Int J Pharmacol Sci Invent. 2013; 2:21–6.

  • Nwanjo HU, Ojiako OA. Effect of vitamins E and C on exercise induced oxidative stress. Global J Pure Appl Sci. 2005; 12(2):199–202. https://doi.org/10.4314/gjpas.v12i2.16591

  • Marklund S, Marklund G. Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469–74. PMid: 4215654. https:// doi.org/10.1111/j.1432-1033.1974.tb03714.x

  • Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121–6. https://doi.org/10.1016/S0076-6879(84) 05016-3

  • Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American J Clin Pathol. 1957; 28:53–6. PMid: 13458125. https://doi.org/10.1093/ ajcp/28.1.56

  • Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82:70–7. https://doi.org/10.1016/0003- 9861(59)90090-6

  • Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130–9.

  • Rotruck JT, Pope AL, Ganther HE. Selenium: Biochemical role as a component of Glutathione Peroxidases. Science. 1973; 179(4073):588–90. PMid: 4686466. https://doi. org/10.1126/science.179.4073.588

  • Lowry OH, Rosenbrough AL, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951; 193:265–75.

  • Sadashivam S, Manickam A. Biochemical methods. Vol 2. New Delhi, India: New Age International (P) Limited; 1996.

  • Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite,and (15N) nitrate in biological fluids. Anal Biochem. 1982;126:131–8. https://doi.org/10.1016/0003-2697(82) 90118-X

  • Esterbauer H, Dieber-Rotheneder M, Waeg G, Striegl G, Juergens G. Biochemical structural and functional properties of oxidized low-density lipoprotein. Chem Res Toxicol. 1990; 3(2):77–92. PMid: 2130945. https:// doi.org/10.1021/tx00014a001

  • Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A review. Intl J Pharm Appl. 2010; 1:38–45.

  • Yasui K, Baba A. Therapeutic potential of Superoxide Dismutase (SOD) for resolution of inflammation. Inflamm Res. 2006; 55:359–63. PMid: 17122956. https:// doi.org/10.1007/s00011-006-5195-y

  • Bowler RP, Nicks M, Tran K, Tanner G, Chang LY, Young SK. Extracellular superoxide dismutase attenuates lipopolysaccharide induced neutrophilic inflammation. Am J Respir Cell Mol Biol. 2004; 31:432–9. PMid: 15256385. https://doi.org/10.1165/rcmb.2004-0057OC

  • Joseph A, Li Y, Koo HC, Davis JM, Pollack S, Kazzaz JA. Superoxide dismutase attenuates hyperoxia-induced interleukin-8 induction via AP-1. Free Radic Biol Med. 2008; 45:1143–9. PMid: 18692129. https://doi. org/10.1016/j.freeradbiomed.2008.07.006

  • Porfire AS, Leucuţa SE, Kiss B, Loghin F, Pârvu AE. Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and anti-inflammatory activity in rat model of peritonitis. Pharmacol Rep. 2014; 66:670–6. PMid: 24948070. https://doi.org/10.1016/j. pharep.2014.03.011

  • Perez-Rivero G, Ruiz-Torres MP, Diez-Marques ML, Canela A, Lopez-Novoa JM, Rodriguez-Puyol M. Telomerase deficiency promotes oxidative stress by reducing catalase activity. Free Radic Biol Med. 2008; 45:1243–51. PMid: 18718525. https://doi.org/10.1016/j. freeradbiomed.2008.07.017

  • Ito K, Nakazato T, Yamato K, Miyakawa Y, Yamada T, Hozumi N. Induction of apoptosis in leukemic cells by homovanillic acid derivative, capsaicin, through oxidative stress: Implication of phosphorylation of p53 at Ser-15 residue by reactive oxygen species. Cancer Res. 2004; 64:1071–8. PMid: 14871840. https://doi. org/10.1158/0008-5472.CAN-03-1670

  • Yu L, Wan F, Dutta S, Welsh S, Liu Z, Freundt E. Autophagic programmed cell death by selective catalase degradation. Proc Natl Acad Sci USA, 2006; 103:4952–7. PMid: 16547133 PMCid: PMC1458776. https://doi.org/10.1073/pnas.0511288103

  • Abdalla MY. Glutathione as potential target for cancer therapy; more or less is good? (mini-review). Jordan J Biol Sci. 2011; 4(3):119–24.

  • Nada SA, El-Shamarka ME-S, Omara EA, Abdel-Salam OM. Grape seed extract and Vitamin C combination blocked LPS-induced multiple organ toxicity in mice. ROS. 2019; 7(21):161–75. https://doi.org/10.20455/ ros.2019.827

  • Jahan MS, Vani G, Shyamaladevi CS. Anti-carcinogenic effect of solanum trilobatum in diethylnitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rats. Asian J Biochem. 2011; 6(1):74–81. https://doi.org/10.3923/ajb.2011.74.81

  • Han YJ, Kwon YG, Chung HT. Antioxidant enzymes suppress Nitric Oxide production through the inhibition of NF-kappa B activation: role of H2O2 and Nitric Oxide in inducible Nitric Oxide synthase expression in macrophages. Nitric Oxide. 2001; 5(5):504–13. PMid: 11587565.

  • RH Shih, CY Wang, CM Yang. NF-kappaB signaling pathways in neurological inflammation: A mini review. Front. Mol Neurosci, 2015; 8:77. PMid: 26733801 PMCid:PMC4683208. https://doi.org/10.3389/fnmol. 2015.00077

  • Choi YY, Kim MH, Hong J, Kim SH, Yang WM. Dried ginger (Zingiber officinalis) inhibits inflammation in alipopolysaccharide- inducedmousemodel.Evid-based Complementary Altern Med. 2013; 914563. PMid: 23935687 PMCid: PMC371222 https://doi. org/10.1155/2013/914563

  • Park HS, Jung HY, Park EY, Kim J, Lee WJ, Bae YS. Cutting edge: Direct interaction of TLR4 with NAD(P) H oxidase 4 isozyme is essential for lipopolysaccharide- induced production of reactive oxygen species and activation of NF-kappa B. J Immunol. 2004; 173(6):3589–93.PMid: 15356101. https://doi. org/10.4049/jimmunol.173.6.3589

  • Erridge C, Bennett-Guerrero E, Poxton IR. Structure and function of lipopolysaccharides. Microbes Infect. 2002; 4(8):837–51. https://doi.org/10.1016/ S1286-4579(02)01604-0

  • Kirtikar KR, Basu BD. Indian medicinal plants Vol. 2. Dehradun India: International Book Distributors; 1984.

  • Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem. 1998; 46:41137. https://doi.org/10.1021/jf9801973

  • Khatri A, Rathore A, Patil UK. Assessment of anti- inflammatory activity of bark of Prosopis cineraria (L.) Druce. Int J Pharm Res. 2012; 4(2):27–9.

  • Sachdeva S, Kaushik V, Saini V. A review on phytochemical and pharmacological potential of Prosopis cineraria. Int J Ethnobiol Ethnomed. 2014; 1(1):1–4.

  • Rotelli AE, Guardia T, Juarez AO, de la Rocha NE, Pelzer LE. Comparative study of flavonoids in experimental models of inflammation. Pharmacol Res. 2003; 48:601–6. https://doi.org/10.1016/S1043-6618(03)00225-1

  • Galvez J, de la Cruz JP, Zarzuelo A, Sanchez de Medina FJ, Jimenez J Sanchez, de la Cuesta F. Oral administration of quercitrin modifies intestinal oxidative status in rats. Gen Pharmacol. 1994; 25:1237– 43. https://doi.org/10.1016/0306-3623(94)90143-0

  • Comalada M, Camuesco D, Sierra S, Ballester I, Xaus J, Galvez J, Zarzuelo A. In vivo quercetin anti- inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-kappaB pathway. Eur J Immunol. 2005; 35:584–92. PMid: 15668926. https://doi.org/10.1002/eji.200425778

  • Sawatzky D, Willoughby D, Colville-Nash P, Rossi A. The involvement of the apoptosis-modulating proteins Erk 1/2, Bcl-xL and Bax in the resolution of acute inflammation in vivo. Am J Pathol. 2006; 168:33–41. PMid: 16400007 PMCid: PMC1592663. https://doi. org/10.2353/ajpath.2006.050058


Abstract Views: 160

PDF Views: 0




  • Isolation, Characterization and Elucidation of Invigorative Potential of Flavonoid from Stem-bark of Prosopis Cineraria on Lps-induced Oxidative Stress and Inflammatory Cascade in Swiss Albino Male Mice

Abstract Views: 160  |  PDF Views: 0

Authors

Veena Sharma
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan, India
Preeti Sharma
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – 304022, Rajasthan, India

Abstract


The present research aimed to elucidate the structure and characterize the isolated compound from stem-bark of Prosopis cineraria and unravel its potential against LPS-induced toxicity in mouse model. The spectral techniques were done for characterization and structure elucidation of the isolated compound (HPLC, NMR, FT-IR, LC-MS. The experimental mice were intoxicated (intra-peritoneal) with LPS (2 mg/kg body weight) and further treated with isolated compound from Prosopis cineraria (15 mg/kg body weight). Dexamethasone was used as a standard (10 mg/kg body weight). The oxidative stress parameters (LPO, CAT, SOD, GSH, GST and GPx) and biochemical activities (AST, ALT, ACP and ALP) were studied. The levels of pro-inflammatory cytokines (TNF-α; Prostaglandins E2; IL-6; NF-κBp65; IFN-γ and IL-10) were determined in liver homogenate. Nitric Oxide (NO) produced due to LPS-intoxication was determined by using Griess reagent. The results of the spectral analysis were used to elucidate the structure of the isolated flavonoid. The isolated flavonoid suppressed the over-expression and altered levels of oxidative parameters and cytokines due to LPS intoxication and restored the levels of TNF-α, NF-κB, NO, IL-6, IFN- , Prostaglandin E2 and IL-10. The research investigation unfolded the alleviating potential of the isolated compound against LPS-induced adverse effects by modulating the expression of cytokines and combating oxidative stress.

Keywords


Anti-inflammatory, Cytokines, Flavonoids, Oxidative Stress, Prosopis cineraria, ROS

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i3%264%2F25615