Toxicology International (Formerly Indian Journal of Toxicology)

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Rutin a Citrus Flavonoid Attenuates Sodium Arsenate Induced Cytotoxicity in CHO Cells


Affiliations
1 Department of Biotechnology, MIT, MAHE, Manipal – 576104, Karnataka,, India
2 Department of Chemistry, MIT, MAHE, Manipal – 576104, Karnataka,, India
3 Department of Biochemistry, KMC, MAHE, Manipal – 576104, Karnataka,, India
     

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The potential role of oxidative stress in the pathogenesis induced by arsenic proposes that antioxidants can be deliberated as an alternative approach in alleviating arsenic-induced toxicity. The aim of this work was assay in vitro the potential advantageous effects of plant flavonoid rutin against arsenate exposure, by studying lipid peroxidation and the stress-related markers. Rutin, a flavanol, has been exhibiting anti-cancer property, mitigating, hostile to diabetic and anti-inflammatory properties. CHO cells were selected to study the effect sodiumarsenate and its amelioration by rutin a citrus flavonoid glycoside. Sodium arsenate at low concentration induced cytotoxicity and genotoxicity. MTT assay, Catalase, Superoxide dismutase, GSH, GST and lipid peroxidation was measured in CHO cell. Pre-treatment of CHO cells with rutin attenuated arsenate induced toxicity. DNA damage was also measured

Keywords

CHO, GSH, GST, SOD, Sodium Arsenate.
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  • Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT-Food Sci Technol. 2008; 41(6):1060–6. https://doi.org/10.1016/j.lwt.2007.06.010

  • Jiang P, Burczynski F, Campbell C, Pierce G, Austria JA, Briggs CJ. Rutin and flavonoid contents in three buckwheat species Fagopyrum esculentum, F. tataricum and F. homotropicum and their protective effects against lipid peroxidation. Food Res Inter. 2007; 40(3):356–64. https://doi.org/10.1016/j.foodres.2006.10.009

  • Ganeshpurkar A, Saluja AK. The pharmacological potential of rutin. Saudi Pharmaceut J. 2017 Feb; 25(2):149–64. PMid: 28344465 PMCid: PMC5355559. https://doi.org/10.1016/j.jsps.2016.04.025

  • Guo X, Yao H, Chen Z. Effect of heat, rutin and disulfide bond reduction on in vitro pepsin digestibility of Chinese tartary buckwheat protein fractions. Food Chem. 2007; 102(1):118–22. https://doi.org/10.1016/j.foodchem. 2006. 04.039

  • Kalgaonkar S, Gross HB, Yokoyama W, Keen CL. Effects of a flavonol-rich diet on select cardiovascular parameters in a Golden Syrian hamster model. J Medicinal Food. 2010; 13(1):108–15. PMid: 20136443. https://doi.org/ 10.1089/jmf.2008.0295

  • Sladowski D, Steer SJ, Clothier RH, Balls M. An improved MIT assay. J Immunol Methods. 1993; 157(1-2):203–7. https://doi.org/10.1016/0022-1759(93)90088-O

  • Aebi H. Catalase in vitro. Methods in enzymology. Academic Press. 1984; 105:121–6. https://doi.org/10 .1016/S0076-6879(84)05016-3

  • Beyer Jr WF, Fridovich I. Phosphate, not superoxide dismutase, facilitates electron transfer from ferrous salts to cytochrome c. Arch Biochem Biophysics. 1991; 285(1):60–3. https://doi.org/10.1016/0003-9861(91)90328-G

  • Grunert RR. A modification of nitroprusside method of analysis for glutathione. Ach Biochem Biophys.1951; 30(2):217–25.

  • Habig WH, Jakoby WB. Assays for differentiation of glutathione S-transferases. Methods in Enzymology. 1981; 77:398–405. https://doi.org/10.1016/S0076-6879 (81)77053-8

  • Gelvan D, Saltman P. Different cellular targets for Cu-and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbituric acid assay. Biochimica et Biophysica Acta (BBA)-General Subjects. 1990; 1035(3):353–60. PMID 2119808. https://doi.org/10.1016/0304-4165(90)90100-B

  • Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1):184–91. https://doi.org/10.1016/0014-4827(88)90265-0

  • Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev. 2016; 3164734. PMid: 26881021 PMCid: PMC4736974. https://doi.org/10.1155/2016/3164734

  • Buddi R, Lin B, Atilano SR, Zorapapel NC, Kenney MC, Brown DJ. Evidence of oxidative stress in human corneal diseases. J Histochem Cytochem. 2002; 50(3): 341–51. PMid: 11850437. https://doi.org/10.1177/002215540205 000306

  • Ayala A, Munoz MF, Arguelles S. Lipid peroxidation: production, metabolism and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014. p. 31. PMid: 24999379 PMCid: PMC4066722. https://doi.org/10.1155/2014/360438

  • Sil Park C, Li L, Lau BH. Thymic peptide modulates glutathione redox cycle and antioxidant enzymes in macrophages. J Leukocyte Biol. 1994; 55(4):496–500. PMID 8145020. https://doi.org/10.1002/jlb.55.4.496

  • Susan A, Rajendran K, Sathyasivam K, Krishnan UM. An overview of plant-based interventions to ameliorate arsenic toxicity. Biomed Pharmacother. 2019; 109:838–52. PMID: 30551538. https://doi.org/10.1016/j.biopha.2018. 10.099

  • Ghaffar A, Hussain R, Abbas G, Ahmad MN, Abbas A, Rahim Y, Younus M, Shahid M, Mohiuddin M. Sodium arsenate and/or urea differently affect clinical attributes, hemato-biochemistry and DNA damage in intoxicated commercial layer birds. Toxin Reviews. 2018 Jul; 37(3):206–15. https://doi.org/10.1080/15569543.20 17.1342096


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  • Rutin a Citrus Flavonoid Attenuates Sodium Arsenate Induced Cytotoxicity in CHO Cells

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Authors

S. A. Salmataj
Department of Biotechnology, MIT, MAHE, Manipal – 576104, Karnataka,, India
Pushpanjali Bhat
Department of Chemistry, MIT, MAHE, Manipal – 576104, Karnataka,, India
Shobha Kamath
Department of Biochemistry, KMC, MAHE, Manipal – 576104, Karnataka,, India

Abstract


The potential role of oxidative stress in the pathogenesis induced by arsenic proposes that antioxidants can be deliberated as an alternative approach in alleviating arsenic-induced toxicity. The aim of this work was assay in vitro the potential advantageous effects of plant flavonoid rutin against arsenate exposure, by studying lipid peroxidation and the stress-related markers. Rutin, a flavanol, has been exhibiting anti-cancer property, mitigating, hostile to diabetic and anti-inflammatory properties. CHO cells were selected to study the effect sodiumarsenate and its amelioration by rutin a citrus flavonoid glycoside. Sodium arsenate at low concentration induced cytotoxicity and genotoxicity. MTT assay, Catalase, Superoxide dismutase, GSH, GST and lipid peroxidation was measured in CHO cell. Pre-treatment of CHO cells with rutin attenuated arsenate induced toxicity. DNA damage was also measured

Keywords


CHO, GSH, GST, SOD, Sodium Arsenate.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i3%264%2F26077