Toxicology International (Formerly Indian Journal of Toxicology)

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Rutin a Citrus Flavonoid Attenuates Sodium Arsenate Induced Cytotoxicity in CHO Cells


Affiliations
1 Department of Biotechnology, MIT, MAHE, Manipal – 576104, Karnataka,, India
2 Department of Chemistry, MIT, MAHE, Manipal – 576104, Karnataka,, India
3 Department of Biochemistry, KMC, MAHE, Manipal – 576104, Karnataka,, India
     

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The potential role of oxidative stress in the pathogenesis induced by arsenic proposes that antioxidants can be deliberated as an alternative approach in alleviating arsenic-induced toxicity. The aim of this work was assay in vitro the potential advantageous effects of plant flavonoid rutin against arsenate exposure, by studying lipid peroxidation and the stress-related markers. Rutin, a flavanol, has been exhibiting anti-cancer property, mitigating, hostile to diabetic and anti-inflammatory properties. CHO cells were selected to study the effect sodiumarsenate and its amelioration by rutin a citrus flavonoid glycoside. Sodium arsenate at low concentration induced cytotoxicity and genotoxicity. MTT assay, Catalase, Superoxide dismutase, GSH, GST and lipid peroxidation was measured in CHO cell. Pre-treatment of CHO cells with rutin attenuated arsenate induced toxicity. DNA damage was also measured

Keywords

CHO, GSH, GST, SOD, Sodium Arsenate.
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  • Rutin a Citrus Flavonoid Attenuates Sodium Arsenate Induced Cytotoxicity in CHO Cells

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Authors

S. A. Salmataj
Department of Biotechnology, MIT, MAHE, Manipal – 576104, Karnataka,, India
Pushpanjali Bhat
Department of Chemistry, MIT, MAHE, Manipal – 576104, Karnataka,, India
Shobha Kamath
Department of Biochemistry, KMC, MAHE, Manipal – 576104, Karnataka,, India

Abstract


The potential role of oxidative stress in the pathogenesis induced by arsenic proposes that antioxidants can be deliberated as an alternative approach in alleviating arsenic-induced toxicity. The aim of this work was assay in vitro the potential advantageous effects of plant flavonoid rutin against arsenate exposure, by studying lipid peroxidation and the stress-related markers. Rutin, a flavanol, has been exhibiting anti-cancer property, mitigating, hostile to diabetic and anti-inflammatory properties. CHO cells were selected to study the effect sodiumarsenate and its amelioration by rutin a citrus flavonoid glycoside. Sodium arsenate at low concentration induced cytotoxicity and genotoxicity. MTT assay, Catalase, Superoxide dismutase, GSH, GST and lipid peroxidation was measured in CHO cell. Pre-treatment of CHO cells with rutin attenuated arsenate induced toxicity. DNA damage was also measured

Keywords


CHO, GSH, GST, SOD, Sodium Arsenate.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i3%264%2F26077