Toxicology International (Formerly Indian Journal of Toxicology)

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Evaluation of the Antibacterial Activity of Isolated Anthraquinones from the Root of Rubia Cordifolia Linn


Affiliations
1 Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh,, India
2 Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
3 Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
     

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The present study was intended to screen the antibacterial activity of anthraquinones i.e. 2-Methyl Anthraquinone (MAQ) and Quinizarine (QNZ), isolated from roots of Rubia cordifolia Linn. Minimum Inhibitory Concentration (MIC) of isolated anthraquinones was evaluated against selected gram-positive [Bacillus subtilis (BS) and Staphylococcus aureus (SA)] and gram-negative [Serratia marcescens (SM) and Escherichia coli (EC)] bacterial strains. The MIC was determined by micro broth dilution method using Tryptone Soya Broth (TSB) in 96 well plates as well as by measuring zone of inhibition using nutrient agar medium by the cup-plate method. Absorbance values after micro broth dilution revealed the significant MIC of MAQ as 40 μg/mL against BS, 40 μg/mL of MAQ against SA and 80 μg/mL of MAQ against SM. Whereas, The MIC of QNZ was 20 μg/mL against EC. Percentage zone of inhibition after cup-plate method revealed the significant MIC of MAQ as 39.8% against BS when compared with vancomycin, 88.33% against SA when compared with linezolid, 53.37% against SM when compared with amikacin. Whereas, percentage zone of inhibition revealed the significant MIC of QNZ as 53.76% against EC when compared with ciprofloxacin. Based on the MIC results after both micro broth dilution and cup-plate method, we observed that MAQ has shown considerable antibacterial activity against selected bacteria.

Keywords

MIC, Micro Broth Dilution, Quinizarine, 2-Methyl Anthraquinone.
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  • Evaluation of the Antibacterial Activity of Isolated Anthraquinones from the Root of Rubia Cordifolia Linn

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Authors

Pottella Srinivasulu
Acharya Nagarjuna University, Guntur – 522510, Andhra Pradesh,, India
Padarthi Pavan Kumar
Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
Ch Aruna Kumar
Department of Physiology and Pharmacology, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
J. Venkateswara Rao
Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
S. Vidyadhara
Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India
Janga Ramesh Babu
Department of Pharmaceutics, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram – 522019, Andhra Pradesh,, India

Abstract


The present study was intended to screen the antibacterial activity of anthraquinones i.e. 2-Methyl Anthraquinone (MAQ) and Quinizarine (QNZ), isolated from roots of Rubia cordifolia Linn. Minimum Inhibitory Concentration (MIC) of isolated anthraquinones was evaluated against selected gram-positive [Bacillus subtilis (BS) and Staphylococcus aureus (SA)] and gram-negative [Serratia marcescens (SM) and Escherichia coli (EC)] bacterial strains. The MIC was determined by micro broth dilution method using Tryptone Soya Broth (TSB) in 96 well plates as well as by measuring zone of inhibition using nutrient agar medium by the cup-plate method. Absorbance values after micro broth dilution revealed the significant MIC of MAQ as 40 μg/mL against BS, 40 μg/mL of MAQ against SA and 80 μg/mL of MAQ against SM. Whereas, The MIC of QNZ was 20 μg/mL against EC. Percentage zone of inhibition after cup-plate method revealed the significant MIC of MAQ as 39.8% against BS when compared with vancomycin, 88.33% against SA when compared with linezolid, 53.37% against SM when compared with amikacin. Whereas, percentage zone of inhibition revealed the significant MIC of QNZ as 53.76% against EC when compared with ciprofloxacin. Based on the MIC results after both micro broth dilution and cup-plate method, we observed that MAQ has shown considerable antibacterial activity against selected bacteria.

Keywords


MIC, Micro Broth Dilution, Quinizarine, 2-Methyl Anthraquinone.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i3%264%2F26169