Toxicology International (Formerly Indian Journal of Toxicology)

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Effects of Caffeine on the Fertility of Hermetia Illucens (Stratiomyidae), Fed with Coffee Pulp


Affiliations
1 Department of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle, Santiago de Cali - 760032,, Colombia
     

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Coffee Pulp is a residue with high concentrations of caffeine, which is a harmful alkaloid that affects fertility in Diptera. The main objective of this study is to define how the caffeine present in the Coffee Pulp affects the fertility of H. illucens, seeking to establish if the bioconversion of Coffee Pulp affects the long-term reproduction of adults. For this purpose, larvae were fed with fresh Coffee Pulp and solid vegetable waste. The pre-pupae obtained were weighed and the internal genitalia of 90 females from each diet were dissected. Afterwards, crosses were conducted between the adults from each diet, the egg clutches were weighed and the emerged larvae were counted. We encountered that the Coffee Pulp does not affect the adults’ survival or the weight of the pre-pupae, but it does affect the developmental duration of the immature state. No significant differences were found in the oocyte production between diets, but their size varied according to the females’ age. We found that the hatching rate varied significantly between the crosses and the control. With this study we can affirm that caffeine does not affect the fertility of H. illucens, but we cannot affirm that it affects the hatching rate. The economic and environmental impact of our results is significant, due to the potential that H. illucens has in the bioconversion of Coffee Pulp and the commercial use of the byproducts.

Keywords

Black Soldier Fly, Caffeine, Coffee Pulp.
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  • Ocampo O, Álvarez-Herrera, L. Coffee production and consumption trends in Colombia. Apun Cenes.2017; 36(64):139–65. https://doi.org/10.19053/01203053.v36. n64.2017.5419

  • Mazzafera P. Degradation of caffffeine by microorganisms and potential use of decaffffeinated coffffee husk and pulp in animal feeding. Sci Agric. 2002; 59:815–21. https:// doi.org /10.1590/S0103-90162002000400030

  • Noriega SA, Silvia, AR, Garcia M. Chemical composition of Coffee Pulp at different silage times for potential use in animal feed. Zoo Trop. 2009; 27:135–41.

  • Selvamurugan M, Doraisamy P, Maheswari M. An integrated treatment system for coffffee processing wastewater using anaerobic and aerobic process. Ecol Eng. 2010; 36:1686–90. https://doi.org/10.1016/j. ecoleng.2010.07.013

  • Gobbi FP. Biología reproductiva y caracterización morfológica de los estadios larvarios de Hermetia illucens (L., 1758) (Diptera: Stratiomyidae). Bases para su producción masiva en Europa. [PhD thesis]. Alicante: Universidad de Alicante; 2012.

  • Tomberlin JK, Sheppard DC. Factors influencing mating and oviposition of black soldier flies (Diptera: Stratiomyidae) in a colony. J Entomol Sci. 2002; 37:345–52. https://doi. org/10.18474/0749-8004-37.4.345

  • Newton L, Sheppard C, Watson D, Burtle G, Dove R. Using the black soldier fly, Hermetia illucens, as a value-added tool for the management of swine manure. APWMC. 2005; 17:2–17.

  • Srinivasan A, Kesavan P. Reproductive toxicity of caffffeine in Musca domestica. J Toxicol Environ Health. 1979; 2:569–76. PMid: 846005. https://doi.org/ 10.1080/1528739770952945

  • Itoyama M, Bicudo H. Effffects of caffffeine on fecundity, egg-laying capacity, development time and longevity in Drosophila prosaltans. Braz J Genet. 1992; 15:303–21.

  • Laranja AT, Manzatto, AJ, Bicudo, HE. Effffects of caffffeine and used coffffee grounds on biological features of Aedes aegypti (Diptera, Culicidae) and their possible use in alternative control. Genet Mol Biol. 2003; 26:419–29. https://doi.org/10.1590/S1415 -47572003000400004

  • Laranja AT, Manzato AJ, Bicudo HE. Caffffeine effffect on mortality and oviposition in successive generations of Aedes aegypti. Rev Saude Publ. 2006; 40:1112–7. PMid: 17173171. https://doi.org/10.1590/S0034-89102 006000700022

  • Larde G. Investigation on some factors affffecting larval growth in a Coffffee Pulp bed. Biol Wastes. 1989; 30:11–9. https://doi.org/10.1016/0269 -7483(89)90139-0

  • Larde G. Recycling of Coffffee Pulp by Hermetia illucens (Diptera: Stratiomyidae) larvae. Biol Wastes. 1990; 33:307–10. https://doi.org/10.1016/0269-7483(90)90134-E

  • Giraldo M, Rodríguez N, Benavides P. Uso potencial de Hermetia illucens (Linnaeus) (Diptera: Stratyiomidae) para transformación de pulpa de café: Aspectos biológicos. Revista Cenicafé. 2019; 70(2):81–90.

  • Pierre F, Rosell M, Quiroz A Granda Y. Chemical and biological evaluation of Coffffee Pulp compost in Caspito. Andres Eloy Blanco municipality, Lara State, Venezuela. Bioagro. 2009; 21:105–10.

  • Hardenburg RE, Watada AE and Wang CY. Almacenamiento comercial de frutas, legumbres y existencias de floristerías y viveros. Instituto interamericano de cooperación para la agricultura, San José, Costa Rica; 1988.

  • Walker TJ, Wineriter SA. Marking techniques for recognizing individual insects. Fla Entomol. 1981; 64: 18–29. https://doi.org/10.2307/3494598

  • Tomberlin JK, Sheppard DC, Joyce JA. Selected life-history traits of black soldier flies (Diptera: Stratiomyidae) reared on three artificial diets. Ann Entomol Soc Am. 2002; 95(3):379–86. https://doi.org/10.1603/0013-8746 (2002)095[0379: SLHTOB]2.0.CO;2

  • R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, [Internet] 2013. http://www.R-project.org/

  • Ulloa JA. Fruits self-stabilized in the container by the obstacle technology. Autonomous University of Nayarit, Tepic, Nayarit, Mexico; 2007.

  • Alattar MA. Biological treatment of leachates of microaerobic fermentation. Dissertation, [Master thesis]. Portland: Portland State University; 2012.

  • Tomberlin JK, Adler PH, Myers HM. Development of the black soldier fly (Diptera: Stratiomyidae) in relation to temperatura. Environ Entomol. 2009; 38:930–4. PMid: 19508804. https://doi.org/10.1603/022.038.0347

  • Sheppard DC, Tomberlin JK, Joyce JA, Kiser BC, Sumner SM. Rearing methods for the black soldier fly (Diptera:Stratiomyidae). J Med Entomol. 2002; 39:695–8. PMid: 12144307. https://doi.org/10.1603/0022-2585-39. 4.695

  • García J, Iannacone J. Viabilidad de huevos y modelo de jaula para la cría artificial masiva de Anastrepha fraterculus (Diptera: Tephritidae). Rev Soc Entomol Arge. 2011; 70(3-4):267–76.

  • Salles LA. Biology of Anastrepha fraterculus. [Internet] 2099. www.iaea.org/inis/collection/NCLCollectionStore/_ Public/30/007/30007856.pdf

  • Brockerhoff EG, Turgeon JJ, Brockerhoff AM. Ovipositional phenology and behavior of the spruce cone fly, Strobilomyia neanthracina (Diptera: Anthomyiidae). J Insect Behav. 1998; 12(1):47–65. https://doi.org/10. 1023/A:1020977114592


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  • Effects of Caffeine on the Fertility of Hermetia Illucens (Stratiomyidae), Fed with Coffee Pulp

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Authors

Nancy Soraya Carrejo Gironza
Department of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle, Santiago de Cali - 760032,, Colombia
Michelle Alexandra Pardo
Department of Biology, Faculty of Natural and Exact Sciences, Universidad del Valle, Santiago de Cali - 760032,, Colombia

Abstract


Coffee Pulp is a residue with high concentrations of caffeine, which is a harmful alkaloid that affects fertility in Diptera. The main objective of this study is to define how the caffeine present in the Coffee Pulp affects the fertility of H. illucens, seeking to establish if the bioconversion of Coffee Pulp affects the long-term reproduction of adults. For this purpose, larvae were fed with fresh Coffee Pulp and solid vegetable waste. The pre-pupae obtained were weighed and the internal genitalia of 90 females from each diet were dissected. Afterwards, crosses were conducted between the adults from each diet, the egg clutches were weighed and the emerged larvae were counted. We encountered that the Coffee Pulp does not affect the adults’ survival or the weight of the pre-pupae, but it does affect the developmental duration of the immature state. No significant differences were found in the oocyte production between diets, but their size varied according to the females’ age. We found that the hatching rate varied significantly between the crosses and the control. With this study we can affirm that caffeine does not affect the fertility of H. illucens, but we cannot affirm that it affects the hatching rate. The economic and environmental impact of our results is significant, due to the potential that H. illucens has in the bioconversion of Coffee Pulp and the commercial use of the byproducts.

Keywords


Black Soldier Fly, Caffeine, Coffee Pulp.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i3%264%2F26234