Toxicology International (Formerly Indian Journal of Toxicology)

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Influence of Vitamin D on Ethanol Induced Retinal Changes in Experimental Rats


Affiliations
1 Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
2 Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
3 Department of Anatomy, American University of Antigua College of Medicine, Coolidge, P.O. Box 1451, Antigua, United States
4 Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
     

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Retina is a neurosensory tissue of ocular system having highly unique lipids which are susceptible to free radical injuries. Chronic ethanol consumption can initiate a wide range of disorders in the CNS and also alteration in the visual system via oxidative pathway. Our study was designed to evaluate the role of vitamin D, a well-known antioxidant and neuroprotective steroid hormone, in the maintenance of ocular health in ethanol induced toxicity. In our present study 4 months old Wistar rats were exposed to ethanol, daily for 6 weeks, while another set of rats received ethanol daily, along with vitamin D for the same duration. A control group consisting of normal rats without any treatment was also included in this experiment. After 6 weeks, Total Anti-Oxidant (TAO) level of eyeball and retinal thickness was measured and statistically analyzed. We observed significantly decreased total antioxidant level in whole eye homogenate in ethanol administered rats which was associated with morphometric changes in the retina. Whereas, vitamin D treated rats showed near to normal retinal morphology as well as TAO level suggesting that vitamin D has the potential to reverse retinal damages caused by ethanol toxicity induced by long term consumption of it.

Keywords

Ethanol, Retina, Toxicity, Vitamin D.
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  • Hassan SM, Saeed AK, Hussein AJ. Ethanol induced hepatic and renal histopathological changes in BALB/c mice. J Nat Sci Res. 2015; 5:2224-86. https://doi.org/10.1155/2015/890632.

  • Hayes DM, Deeny MA, Shaner CA, Nixon K. Determining the threshold for alcohol‐induced brain damage: new evidence with gliosis markers. Alcohol Clin Exp Res. 2013; 37(3):425-34. https://doi.org/10.1111/j.1530-0277.2012.01955.x. PMid:23347220 PMCid:PMC3586303.

  • Blanco A, Guerri C. Alcohol and neuro inflammation: Involvement of astroglial cells and TLR4/IL-1RI receptors. Inmunología. 2006; 25:188-200.

  • Berry KA, Gordon WC, Murphy RC, Bazan NG. Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. Journal of Lipid Research. 2014; 55(3):504-15. https://doi.org/10.1194/jlr.M044990. PMid:24367044 PMCid:PMC3934734.

  • Datta AK. Essentials of human Anatomy - Head and Neck. 3rd ed. Kolkata, Current Books International; 2004.

  • Avignon J, Díaz-Llopis M, Spain E, Johnsen-Soriano S, Romero B, Marín N, Muriach M, Bosch-Morell F, Romero FJ. Chronic ethanol feeding induces oxidative stress in the optic nerve of rats. Archives of the Spanish Society of Ophthalmology. 2002; 77(5): 263-68. PMID: 12023745.

  • Johnsen-Soriano S, Genovés JM, Romero B, García-Delpech S, Muriach M, Sancho-Tello M, et al. Chronic ethanol feeding induces oxidative stress in the rat retina: treatment with the antioxidant ebselen. Arch Soc Esp Oftalmol. 2007; 82(12):757-62. https://doi.org/10.4321/S0365-66912007001200008. PMid:18040919.

  • Matsui JI, Egana AL, Sponholtz TR, Adolph AR, Dowling JE. Effects of ethanol on photoreceptors and visual function in developing zebrafish. Invest Ophthalmol Vis Sci. 2006; 47(10):4589-97. https://doi.org/10.1167/iovs.05-0971. PMid:17003456 PMCid:PMC2408731.

  • Chung HY, Chang CT, Young HW, Hu SP, Tzou WS, Hu CH. Ethanol inhibits retinal and CNS differentiation due to failure of cell cycle exit via an apoptosis-independent pathway. Neurotoxicology and Teratology. 2013; 38:92-103. https://doi.org/10.1016/j.ntt.2013.05.006. PMid:23714372.

  • Morello M, Landel V, Lacassagne E, Baranger K, Annweiler C, Féron F, et al. Vitamin D improves neurogenesis and cognition in a mouse model of Alzheimer’s disease. Molecular Neurobiology. 2018; 55(8):6463-79. https://doi.org/10.1007/s12035-017-0839-1. PMid:29318446 PMCid:PMC6061182.

  • Reins RY, McDermott AM. Vitamin D: Implications for ocular disease and therapeutic potential. Experimental Eye Research. 2015; 134:101-10. https://doi.org/10.1016/j.exer.2015.02.019. PMid:25724179 PMCid:PMC4426046.

  • Lu L, Lu Q, Chen W, Li J, Li C, Zheng Z. Vitamin D3 protects against diabetic retinopathy by inhibiting high-glucose-induced activation of the ROS/TXNIP/NLRP3 inflammasome pathway. Journal of Diabetes Research. 2018. https://doi.org/10.1155/2018/8193523. PMid:29682582 PMCid:PMC5842685.

  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. Journal of Clinical Pathology. 2001; 54(5):356-61. https://doi.org/10.1136/jcp.54.5.356. PMid:11328833 PMCid:PMC1731414.

  • Michon JJ, Li ZL, Shioura N, Anderson RJ, Tso MO. A comparative study of methods of photoreceptor morphometry. Investigative Ophthalmology and Visual Science. 1991; 32(2):280-84. PMID: 1993578.

  • Ferreira JG, Hawkins AJ, Bricker SB. Management of productivity, environmental effects and profitability of shellfish aquaculture-the Farm Aquaculture Resource Management (FARM) model. Aquaculture. 2007; 264(1-4):160-74. https://doi.org/10.1016/j.aquaculture.2006.12.017.

  • Sancho-Tello M, Muriach M, Barcia J, Bosch-Morell F, Genovés JM, Johnsen-Soriano S, et al. Chronic alcohol feeding induces biochemical, histological, and functional alterations in rat retina. Alcohol and Alcoholism. 2008; 43(3):254-60. https://doi.org/10.1093/alcalc/agn006. PMid:18304993.

  • Kusano C, Ferrari B. Total antioxidant capacity: A biomarker in biomedical and nutritional studies. Journal of Cell and Molecular Biology. 2008; 7(1):1-5.

  • Diamond I, Gordon AS. Cellular and molecular neuroscience of alcoholism. Physiological Reviews. 1997; 77(1):1-20. https://doi.org/10.1152/physrev.1997.77.1.1. PMid:9016298.

  • Gupta YK, Gupta M, Kohli K. Neuroprotective role of melatonin in oxidative stress vulnerable brain. Indian Journal of Physiology and Pharmacology. 2003; 47:373-86. PMID: 15266948.

  • Das SK, Hiran KR, Mukherjee S, Vasudevan DM. Oxidative stress is the primary event: effects of ethanol consumption in brain. Indian Journal of Clinical Biochemistry. 2007; 22(1):99. https://doi.org/10.1007/BF02912890. PMid:23105661 PMCid:PMC3454264.

  • Mirzaei A, Mirzaei N, Alamdari A. Effects of chronic ethanol consumption on biochemical parameters and oxidative stress on rat. Indian Journal of Science and Technology. 2015; 8(25):1-5. https://doi.org/10.17485/ijst/2015/v8i25/59160.

  • Bosch-Morell F, Martı́nez-Soriano F, Colell A, Fernández-Checa JC, Romero FJ. Chronic ethanol feeding induces cellular antioxidants decrease and oxidative stress in rat peripheral nerves. Effect of S-adenosyl-L-methionine and N-acetyl-L-cysteine. Free Radical Biology and Medicine. 1998; 25(3):365-68. https://doi.org/10.1016/S0891-5849(98)00036-7.

  • Luo XG, Chiu K, Lau FH, Lee VW, Yung KK, So KF. The selective vulnerability of retinal ganglion cells in rat chronic ocular hypertension model at early phase. Cellular and molecular neurobiology. 2009; 29(8):1143. https://doi.org/10.1007/s10571-009-9407-1. PMid:19396539.

  • Hernández M, Urcola JH, Vecino E. Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments. Experimental eye research. 2008; 86(5):798-806. https://doi.org/10.1016/j.exer.2008.02.008. PMid:18394603.

  • Chan HC, Chang RC, Ip AK, Chiu K, Yuen WH, Zee SY, et al. Neuroprotective effects of Lyciumbarbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma. Experimental neurology. 2007; 203(1):269-73. https://doi.org/10.1016/j.expneurol.2006.05.031. PMid:17045262.

  • Sabet SJ, Darjatmoko SR, Lindstrom MJ, Albert DM. Antineoplastic effect and toxicity of 1, 25-dihydroxy-16-ene-23-yne-vitamin D3 in athymic mice with Y-79 human retinoblastoma tumors. Archives of ophthalmology. 1999; 117(3):365-70. https://doi.org/10.1001/archopht.117.3.365. PMid:10088815.

  • Graffe A, Annweiler C, Mauget‐Faÿsse M, Beauchet O, Kodjikian L, Milea D. Association Between Hypovitaminosis D and Late Stages of Age‐Related Macular Degeneration: A Case-Control Study. Journal of the American Geriatrics Society. 2012; 60(7):1367-69. https://doi.org/10.1111/j.1532-5415.2012.04015.x. PMid:22788394.


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  • Influence of Vitamin D on Ethanol Induced Retinal Changes in Experimental Rats

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Authors

Deekshitha Arunachalam
Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
Ashwin R. Rai
Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
Rajalakshmi Rai
Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India
Teresa Joy
Department of Anatomy, American University of Antigua College of Medicine, Coolidge, P.O. Box 1451, Antigua, United States
Shyamala Nayak
Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka,, India

Abstract


Retina is a neurosensory tissue of ocular system having highly unique lipids which are susceptible to free radical injuries. Chronic ethanol consumption can initiate a wide range of disorders in the CNS and also alteration in the visual system via oxidative pathway. Our study was designed to evaluate the role of vitamin D, a well-known antioxidant and neuroprotective steroid hormone, in the maintenance of ocular health in ethanol induced toxicity. In our present study 4 months old Wistar rats were exposed to ethanol, daily for 6 weeks, while another set of rats received ethanol daily, along with vitamin D for the same duration. A control group consisting of normal rats without any treatment was also included in this experiment. After 6 weeks, Total Anti-Oxidant (TAO) level of eyeball and retinal thickness was measured and statistically analyzed. We observed significantly decreased total antioxidant level in whole eye homogenate in ethanol administered rats which was associated with morphometric changes in the retina. Whereas, vitamin D treated rats showed near to normal retinal morphology as well as TAO level suggesting that vitamin D has the potential to reverse retinal damages caused by ethanol toxicity induced by long term consumption of it.

Keywords


Ethanol, Retina, Toxicity, Vitamin D.

References





DOI: https://doi.org/10.18311/ti%2F2022%2Fv29i1%2F27988