Toxicology International (Formerly Indian Journal of Toxicology)

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Cleistopholis Patens Extracts Attenuate Inflammatory Cytokine and Oxidative Stress Status in Complete Freund’s Adjuvant-induced Rheumatoid Arthritis in Wistar Albino Rats


Affiliations
1 Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State,, Nigeria
2 Department of Biochemistry, Faculty of Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
3 Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
4 Department of Physiology, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria
5 Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State,
6 Department of Biochemistry, Delta State University, Abraka,, Nigeria
     

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Rheumatoid Arthritis (RA) is an autoimmune inflammatory disease. Accumulating evidences show that natural products use for RA treatment is gaining importance in the medical world due to negative effects associated with orthodox medicines. This study investigated the anti-inflammatory and antioxidant potency of Stem Bark of Cleistopholis Patens (SBCP) on Chicken Type II collagen in Complete Freund’s Adjuvant (CFA) induced RA in rats. Experimental rats were divided into nine groups (n= 15). RA was established in the rats by a single intradermal injection of 0.1ml Chicken Type II collagen in CFA through the left hind paw. The serum concentrations of Inter-interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) were evaluated. Unlike the untreated arthritic group, the test groups (4-9) orally administered with graded doses (400, 600 and 800 mg/kg) of SBCP extracts for twenty-one days showed significant reduction (P < 0.05) in concentrations of IL-1β, IL-6, TNF-α, MDA and NO while the activities of SOD and CAT increased significantly (P < 0.05) when compared to the negative control. The anti-inflammatory and anti-oxidative effects of SBCP extracts were comparable to that of the reference drug, indomethacin. This study indicates that SBCP ethanol and aqueous extracts could suppress proinflammatory cytokines and reduce oxidative stress in rheumatoid arthritic rats.

Keywords

Anti-Inflammatory, Antioxidants, Cleistopholis patens, Oxidative Stress, Rheumatoid Arthritis.
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  • Cleistopholis Patens Extracts Attenuate Inflammatory Cytokine and Oxidative Stress Status in Complete Freund’s Adjuvant-induced Rheumatoid Arthritis in Wistar Albino Rats

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Authors

Chinyere Aloke
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State,, Nigeria
Udu A. Ibiam
Department of Biochemistry, Faculty of Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
Orji U. Obasi
Department of Biochemistry, Faculty of Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
Nwogo A. Obasi
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State,, Nigeria
Chuks O. Edeogu
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
Patrick M. Aja
Department of Biochemistry, Faculty of Sciences, Ebonyi State University, Abakaliki Ebonyi State,, Nigeria
Chinedum U. Emelike
Department of Physiology, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria
Patience N. Ogbu
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State,
Joseph C. Mordi
Department of Biochemistry, Delta State University, Abraka,, Nigeria

Abstract


Rheumatoid Arthritis (RA) is an autoimmune inflammatory disease. Accumulating evidences show that natural products use for RA treatment is gaining importance in the medical world due to negative effects associated with orthodox medicines. This study investigated the anti-inflammatory and antioxidant potency of Stem Bark of Cleistopholis Patens (SBCP) on Chicken Type II collagen in Complete Freund’s Adjuvant (CFA) induced RA in rats. Experimental rats were divided into nine groups (n= 15). RA was established in the rats by a single intradermal injection of 0.1ml Chicken Type II collagen in CFA through the left hind paw. The serum concentrations of Inter-interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) were evaluated. Unlike the untreated arthritic group, the test groups (4-9) orally administered with graded doses (400, 600 and 800 mg/kg) of SBCP extracts for twenty-one days showed significant reduction (P < 0.05) in concentrations of IL-1β, IL-6, TNF-α, MDA and NO while the activities of SOD and CAT increased significantly (P < 0.05) when compared to the negative control. The anti-inflammatory and anti-oxidative effects of SBCP extracts were comparable to that of the reference drug, indomethacin. This study indicates that SBCP ethanol and aqueous extracts could suppress proinflammatory cytokines and reduce oxidative stress in rheumatoid arthritic rats.

Keywords


Anti-Inflammatory, Antioxidants, Cleistopholis patens, Oxidative Stress, Rheumatoid Arthritis.

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DOI: https://doi.org/10.18311/ti%2F2021%2Fv29i1%2F28162