Toxicology International (Formerly Indian Journal of Toxicology)

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The Toxicity of Imidacloprid on Early Embryonic Stages And Growth Rate of Hatchlings of Common Carp, Cyprinus Carpio


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1 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana,, India
     

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In the present investigation, the toxicity of imidacloprid was tested on the embryos (fertilized eggs) and hatchlings of Cyprinus carpio in terms of mortality (%), hatching success (%) and abnormal/malformed hatchling along with their growth performance in toxicant medium. Reported data from acute toxicity tests of imidacloprid was statistically analyzed by Probit Analysis Method and 48 h (hour) LC50 was reported as 78 ppm for embryos of C. carpio. Three sublethal doses of imidacloprid i.e., 7.8 ppm (T1), 15.6 ppm (T2) and 23.4 ppm (T2), were selected for further experimentation along with control. The findings revealed that mortality of exposed eggs (%) was significantly (p<0.05) increased in dose dependent manner as a result hatching rate (%) was decreased as compared to control. Similarly, per cent of abnormal hatchling was also increased significantly (p<0.05) with increase in pesticide concentration. Various types of abnormalities has been reported in different body regions such as whole length curved body, stunted growth, hatchling with single eye, malformed head, flexure in the tail and deformed yolk sac etc. Growth rate was observed as length gain in centimeter (cm), weight gain in grams (g) and growth per cent gain in body weight of hatchling, which was also found to be declined significantly (p<0.05) with increase in pesticide concentration. The findings of the present investigation suggest that imidacloprid has significant effect on the early developmental stages, induces malformations and also decline the growth rate of hatchlings.

Keywords

Cyprinus carpio, Growth Performance, Imidacloprid, Malformations.
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  • Schulz R. Field studies on exposure, effects, and risk mitigation of aquatic nonpoint-source insecticide pollution: A review. J. Environ. Qual. 2004; 33:419–48. https://doi.org/10.2134/jeq2004.4190. PMid:15074794.

  • Pimentel D. The pesticide hazard: A global health and environmental audition: Compiled by Barbara Dinham. London: Zed Books Ltd. Agricultural Systems. 1994; 45:470–72. https://doi.org/10.1016/0308-521X(94)90137-5

  • Edwards CA. Pesticide residues in soil and water. In: Environmental pollution by pesticides (Edwards, C.A., Ed.). London: Plenum Press; 1973. https://doi.org/10.1007/978-1-4615-8942-6_12. PMid:4124892.

  • Brown AWA. Ecology of Pesticides. New York: NY: John Wiley & Sons, Inc; 1978.

  • Schulz R. Field studies on exposure, effects and risk mitigation of aquatic nonpoint source insecticide pollution. J Environ Qual. 2004; 33:419–48. https://doi.org/10.2134/jeq2004.4190. PMid:15074794.

  • Villalobos SA, Hamm JT, Teh SJ, Hinton DE. Thiobencarb-induced embryotoxicity in medaka (Oryzias latipes): stage-specific toxicity and the protective role of chorion. Aquat Toxicol. 2000; 48:309–26. https://doi.org/10.1016/S0166-445X(99)00032-6.

  • Cameron P, Berg J, Dethlefsen V, Westernhagen HV. Developmental defects in pelagic embryos of several flatfish species in the southern North Sea. Neth J Sea Res. 1992; 29:239–56. https://doi.org/10.1016/0077-7579(92)90024-9.

  • Bhardwaj JK, Harkrishan, Tyor AK. Imidacloprid Induced Alterations in Behavioral and Locomotory Activity of Fingerlings of Common Carp, Cyprinus carpio. Toxicol Int. 2020; 27:158–67.

  • Bhardwaj JK, Harkrishan, Tyor AK. Sublethal effects of imidacloprid on Haematological and biochemical profile of Freshwater fish, Cyprinus carpio. J Adv Zool. 2020; 41:75–88.

  • Nagel R. DarT: the embryo test with the zebrafish, Danio rerio a general model in ecotoxicology and toxicology. Altex. 2002; 19:38–48.

  • Westernhagen HV. Sublethal effects of pollutants on fish eggs and larvae. In: Hoar WS, Randall DJ, eds. Fish Physiology: The Physiology of Developing Fish. Harcourt Brace Jova. Academic Press, INC; 1988. https://doi.org/10.1016/S1546-5098(08)60201-0.

  • Stasiunaite P. Long-term heavy metal mixture toxicity to embryos and Alevins of rainbow trout (Oncorhynchus mykiss). Acta Zool Litu. 1999; 2:40–5. https://doi.org/10.1080/13921657.1999.10512286.

  • Koprucu K, Aydın R. The toxic effects of pyrethroid deltamethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Pestic Biochem Physiol. 2004; 8:47–53. https://doi.org/10.1016/j.pestbp.2004.05.004.

  • Lammer E, Carr GJ, Wendler K, Rawlings JM, Belanger SE, Braunbeck T. Is the fish embryo toxicity test (FET) Zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test? Comp Biochem Physiol, Part C. 2009; 149:196–209. https://doi.org/10.1016/j.cbpc.2008.11.006. PMid:19095081.

  • Sarkar M, Biswas P, Roy S, Kole R, Chowdhury A. Effect of pH and type of formulation on the persistence of imidacloprid in water. Bull Environ Contam Toxicol. 1999; 63:604–9. https://doi.org/10.1007/s001289901023. PMid:10541679.

  • Matsuda K, Buckingham SD, Kleier D, Rauh J, Grauso M, Sattelle DB. Neonicotinoids: insecticides acting on insect nicotinic acetylcholine receptors. Trends Pharmacol Sci. 2001; 22:73–80. https://doi.org/10.1016/S0165-6147(00)01820-4.

  • Kagabu S, Medej S. Stability comparison of imidacloprid and related compounds under simulated sunlight hydrolysis conditions and to oxygen. Biosci Biotechnol Biochem. 1995; 59:980–85. https://doi.org/10.1271/bbb.59.980.

  • Kreuger J, Graaf S, Patring J. Pesticides in surface water in areas with open ground and greenhouse horticultural crops in Sweden 2008. Ekohydrologi. 2010:1–49.

  • Hladik ML, Kolpin DW. First national-scale reconnaissance of Neonicotinoid insecticides in streams across the USA. Environ Chem. 2015; 13:12–20. https://doi.org/10.1071/EN15061.

  • Annon. EPA bans most DDT uses, readies lead action. Environ. Sci. Technol. 1972; 6:675.

  • Sanyal N, Hazra D, Pal R, Somchaudhury AK, Chowdhury A. Imidacloprid in processed tea and tea liquor. J Zhejiang Univ Sci B. 2006; 7:619–22. https://doi.org/10.1631/jzus.2006.B0619. PMid:16845714. PMCid:PMC1533760.

  • OECD Guideline for testing of chemicals. Test Guideline 210, Fish, Early life Stage Toxicity Test [Internet]. 1992. Available from: https://www.oecd.org/chemicalsafety/risk-assessment/1948269.pdf.

  • Jezierska B, Lugowska K, Witeska M, Sarnowski P. Malformations of newly hatched common carp larvae. Electron J Pol AgricUniv. 2000; 3:1–5.

  • Garg SK, Bhatnagar A, Kalla A, Johal MS. Experimental ichthyology. New Delhi: CBS Publishers; 2002.

  • Spacie A, Hamelink J. Bioaccumulation fundamentals of aquatic toxicology: Methods and applications. Washington DC: Hemisphere Publishing Corporation; 1985.

  • Hilmy AM, Shabana MB, Daabees AY. Bioaccumulation of cadmium: toxicity in Mugil cephalus. Comp Biochem Physiol, Part C. 1985; 81:139–44. https://doi.org/10.1016/0742-8413(85)90105-7.

  • Islam MA, Hossen MS, Sumon KA, Rahman MM. Acute toxicity of imidacloprid on the developmental stages of common carp Cyprinus carpio. Toxicol Environ Health Sci. 2019; 11:244–51. https://doi.org/10.1007/s13530-019-0410-8.

  • Aydin R, Koprucu K. Acute toxicity of diazinon on the common carp (Cyprinus carpio L.) embryos and larvae. Pestic Biochem Physiol. 2005; 82:220–25. https://doi.org/10.1016/j.pestbp.2005.03.001.

  • Richterva Z, Machova J, Stara A, Tumova J, Velisek J, Sevcikova M, et al. Effects of a cypermethrin-based pesticide on early life stages of common carp (Cyprinus carpio L.). Veterinary Medicine. 2015; 60:423–31. https://doi.org/10.17221/8417-VETMED.

  • Birge WJ, Black JA, Westerman AG, Ramey BA. Fish and amphibian embryos a model system for evaluating teratogenicity. Fundam Appl Toxicol. 1983; 3:237–42. https://doi.org/10.1016/S0272-0590(83)80134-1.

  • Ansari S, Ansari BA. Embryo and Fingerling toxicity of dimethoate and effect on fecundity, viability, hatchability and survival of zebrafish, Danio rerio (Cyprinidae). World Journal of Fish and Marine Sciences. 2011; 3:167–73.

  • Hagenmaier H. The hatching process in fish embryos: V. Characterization of the hatching protease (chorionase) from the perivitelline fluid of the rainbow trout, Salmo gairdneri Rich, as a metalloenzyme. Roux’s Arch Dev Biol. 1974; 175:157–62. https://doi.org/10.1007/BF00574299. PMid:28304719.

  • Ansari BA, Aslam M, Kumar K. Diazinon toxicity: activities of acetylcholinesterase and phosphatases in the nervous tissues of zebra fish, Brachydanio rerio (Cyprinidae). Acta Hydrochim Hydrobiol. 1987; 15:301–5. https://doi.org/10.1002/aheh.19870150309.

  • Walker MK, Peterson RE. Potencies of polychlorinated dibenzo-p-dioxin, dibenzofuran, and biphenyl congeners, relative to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin, for producing early life stage mortality in rainbow trout (Oncorhynchus mykiss). Aquat Toxicol. 1991; 21:219–38. https://doi.org/10.1016/0166-445X(91)90074-J.

  • Zorriehzahra MJ. Aetiologic agents of fry mortality syndrome in the rainbow trout (Oncorhynchus mykiss) in Iran. Ph.D. Thesis: University of Putra, Malaysia; 2008. 36. Velisek J, Stara A. Effect of thiacloprid on earl life stages of common carp (C. carpio). Chemosphere. 2018; 194:481–7. https://doi.org/10.1016/j.chemosphere.2017.11.176. PMid:29232641.

  • Kanazawa J. Bioconcentration ratio of diazinon by freshwater fish and snail. Bull Environ Contam Toxicol. 1978; 20:613–17. https://doi.org/10.1007/BF01683573. PMid:737338.

  • Kuder RS, Gundala HP. Developmental toxicity of deltamethrin and 3-phenoxybenzoic acid in embryo-larval stages of zebrafish (Danio rerio). Toxicol Mech Methods. 2018; 28:415–22. https://doi.org/10.1080/15376516.2018.1439131. PMid:29421951.

  • Ekrem SC, Hasan K, Sevdan Y. Effects of phosalone on mineral contents and spinal deformities in common carp (Cyprinus carpio L.). Turkish J Fish Aquat Sci. 2012; 12:259–64.

  • Gorge G, Nagel R. Toxicity of lindane, atrazine and deltamethrin to early life stages of zebrafish (Brachydanio rerio). Ecotoxicol Environ Saf. 1990; 20:246–55. https://doi.org/10.1016/0147-6513(90)90004-O.

  • Guimares AT, Silva de Assis HC, Boeger W. The effect of trichlorfon on acetylcholinesterase activity and histopathology of cultivated fish Oreochromis niloticus. Ecotoxicol Environ Saf. 2007; 68:57–62. https://doi.org/10.1016/j.ecoenv.2006.08.005. PMid:17055053.

  • Gagnon MM, Rawson CA. Diuron increases spinal deformity in early life stage of pink snapper Pagrus auratus. Mar Pollut Bull. 2009; 58:1078–95. https://doi.org/10.1016/j.marpolbul.2009.04.011. PMid:19427652.

  • Kienle C, Kohler HR, Gerhardt A. Behavioural and developmental toxicity of chlorpyrifos and nickel chloride to zebrafish (Danio rerio) embryos and larvae. Ecotoxicol Environ Saf. 2009; 72:1740–7. https://doi.org/10.1016/j.ecoenv.2009.04.014. PMid:19477011.

  • Ponmani RB, Logaswamy S. Sub-lethal effect of monocropotophos on food utilization of Cyprinus carpio. J Environ Biol. 1997; 18:321–4.

  • Harkrishan, Saraf P, Tyor AK, Bhardwaj JK. Effect of Imidacloprid on Histopathological Alterations of Brain, Gills and Eyes in hatchling carp (Cyprinus carpio L.). Toxicol Int. 2020; 27:70–8.

  • Sweilum MA. Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticus L.) and water quality of ponds. Aquac Res. 2006; 37:1079–89. https://doi.org/10.1111/j.1365-2109.2006.01531.x.

  • Majumder R, Kaviraj A. Cypermethrin induced stress and changes in growth of freshwater fish Oreochromis niloticus. Int Aquat Res. 2017; 9:117–28. https://doi.org/10.1007/s40071-017-0161-6.

  • Agbohessi TP, Toko II, Ntcha I, Geay F, Mandiki SNM, Kestemont P. Exposure to agricultural pesticides impairs growth, feed utilization and energy budget in African catfish Clarias gariepinus (Burchell, 1822) fingerlings.Int Aquat Res. 2014; 6:229–43. https://doi.org/10.1007/s40071-014-0083-5.

  • Majumder R, Kaviraj A. Acute and sublethal effects of organophosphate insecticide chlorpyrifos on freshwater fish Oreochromis niloticus. Drug Chem Toxicol. 2018; 26:1–9. https://doi.org/10.1080/01480545.2018.1425425. PMid:29372658.

  • Lal B, Sarang MK, Kumar P. Malathion exposure induces the endocrine disruption and growth retardation in the catfish, Clarias batrachus (Linn). Gen Comp Endocrinol. 2013; 181:139–45. https://doi.org/10.1016/j.ygcen.2012.11.004. PMid:23174696.

  • Dube PN, Hosetti BB. Behaviour surveillance and oxygen consumption in the freshwater fish Labeo rohita (Hamilton) exposed to sodium cyanide. Biotechnol Anim Husb. 2010; 26:91–103. https://doi.org/10.2298/BAH1002091D.

  • Jin Y, Wu S, Zeng Z, Fu Z. Effects of environmental pollutants on gut microbiota. Environ Pollut. 2017; 222:1–9. https://doi.org/10.1016/j.envpol.2016.11.045. PMid:28086130.

  • Ganeshwade RM. Effect of pesticides on freshwater fishes from Marathwada region. Ph.D. Thesis, Dr. Babasaheb Ambedkar Marathwada University; Aurangabad, India; 2002.


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  • The Toxicity of Imidacloprid on Early Embryonic Stages And Growth Rate of Hatchlings of Common Carp, Cyprinus Carpio

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Authors

Jitender Kumar Bhardwaj
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana,, India
Harkrishan Kamboj
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana,, India
Anil K. Tyor
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana,, India

Abstract


In the present investigation, the toxicity of imidacloprid was tested on the embryos (fertilized eggs) and hatchlings of Cyprinus carpio in terms of mortality (%), hatching success (%) and abnormal/malformed hatchling along with their growth performance in toxicant medium. Reported data from acute toxicity tests of imidacloprid was statistically analyzed by Probit Analysis Method and 48 h (hour) LC50 was reported as 78 ppm for embryos of C. carpio. Three sublethal doses of imidacloprid i.e., 7.8 ppm (T1), 15.6 ppm (T2) and 23.4 ppm (T2), were selected for further experimentation along with control. The findings revealed that mortality of exposed eggs (%) was significantly (p<0.05) increased in dose dependent manner as a result hatching rate (%) was decreased as compared to control. Similarly, per cent of abnormal hatchling was also increased significantly (p<0.05) with increase in pesticide concentration. Various types of abnormalities has been reported in different body regions such as whole length curved body, stunted growth, hatchling with single eye, malformed head, flexure in the tail and deformed yolk sac etc. Growth rate was observed as length gain in centimeter (cm), weight gain in grams (g) and growth per cent gain in body weight of hatchling, which was also found to be declined significantly (p<0.05) with increase in pesticide concentration. The findings of the present investigation suggest that imidacloprid has significant effect on the early developmental stages, induces malformations and also decline the growth rate of hatchlings.

Keywords


Cyprinus carpio, Growth Performance, Imidacloprid, Malformations.

References





DOI: https://doi.org/10.18311/ti%2F2022%2Fv29i1%2F28317