Toxicology International (Formerly Indian Journal of Toxicology)

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Carbosulfan Induced Reproductive Toxicity in Male Albino Rats (Rattus Norvegicus) and Protective Effect of Cissus Quadrangularis


Affiliations
1 Department of Zoology, Karnatak University, Dharwad – 580003, Karnataka,, India
     

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Carbosulfan is the most commonly used carbamate insecticide in the agriculture industry and is known to cause diverse toxicological effects on non-target organisms. Farmers are more susceptible to accidental consumption of insecticides owing to their unscientific use and ease of access. The present investigation was carried out to evaluate reproductive toxicity induced by carbosulfan and its amelioration using traditional Cissus quadrangularis plant extract. The rats were divided into six groups; (E1) control treated with 1% DMSO/kg bw, (E2) plant extract alone (1000 mg/kg bw), (E3) low dose of carbosulfan (3.4 mg/kg bw), (E4) high dose of carbosulfan (6.37 mg/kg bw), (E5) low dose (3.4 mg/kg bw) and (E6) high dose (6.37 mg/kg bw) carbosulfan co-treatment with Cissus quadrangularis extract (1000 mg/kg bw) for 60 days. The reproductive parameters including histology of testes, sperm count, motility, morphology, and hormonal assay (FSH, T, LH) were performed. Carbosulfan treated groups (E3 & E4) were manifested histopathological alterations including tubular degeneration, disorganisation of cells, vacuolisation, and sloughing of germ cells into the tubular lumen. The hormonal level, sperm count, and motility were significantly decreased and the sperm abnormality rate was increased in the E4 group compared to the control group. The groups (E5 & E6) co-treated with plant extract were significantly recovered compared to insecticide-treated groups (E3 & E4). The histological and hormonal changes were attributed to the reprotoxic effects of the insecticide. However, co-treatment of plant extract significantly recovered the toxic effects caused by low concentration (E5) than the high concentration (E6) of carbosulfan.

Keywords

Carbosulfan, Cissus quadrangularis, Plant Extract, Reproductive Toxicity, Testes Histology
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  • Karunarathne A, Gunnell D, Konradsen F, Eddleston M. How many premature deaths from pesticide suicide have occurred since the agricultural Green Revolution? Clin Toxicol. 2020; 58(4):227-232. https://doi.org/10.1080/15563650.2019.1662433. PMid:31500467.

  • Statistical Database, GOI (Internet). 2021. Cited 2021 Jun 3. Available from: http://ppqs.gov.in/statistical-database.

  • Guillet P, N’Guessan R, Darriet F, Traore‐Lamizana M, Chandre F, Carnevale P. Combined pyrethroid and carbamate ‘two-in-one’ treated mosquito nets: field efficacy against pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus. Med Vet Entomol. 2001; 15(1):105-112. https://doi.org/10.1046/j.1365-2915.2001.00288.x. PMid:11297094.

  • Wei J, Furrer G, Schulin R. Kinetics of Carbosulfan Degradation in the Aqueous Phase in the Presence of a Cosolvent. J Environ Qual. 2000; 29(5):1481-1487. https://doi.org/10.2134/jeq2000.00472425002900050015x.

  • Campbell S, David MD, Woodward LA, Li QX. Persistence of carbofuran in marine sand and water. Chemosphere.2004; 54(8):1155-1161. https://doi.org/10.1016/j.chemosphere. 2003.09.018. PMid:14664844.

  • David M, Lokeshkumar P. Studies on carbosulfan induced oxygen deprivation and behavioral anomalies in fresh water fish Cyprinus carpio. J Adv Sci Res. 2020; 11(4 Suppl 9):36-43.

  • Nwani CD, Agrawal ND, Raghuvanshi S, Jaswal A, Shrivastava S, Sinha N, Onyishi G, Shukla S. Toxicological effects of carbosulfan in rats: Antioxidant, enzymological, biochemical, and hematological responses. Toxicol Ind Health. 2016; 32(7):1335-1343. https://doi.org/10.1177/0748233714564243. PMid:25564597.

  • Giri S, Giri A, Sharma GD, Prasad SB. Mutagenic effects of carbosulfan, a carbamate pesticide. Mutat Res Genet Toxicol Environ Mutagen. 2002; 519(1-2):75-82. https://doi.org/10.1016/S1383-5718(02)00114-6.

  • Giri S, Giri A, Sharma GD, Prasad SB. Induction of sister chromatid exchanges by cypermethrin and carbosulfan in bone marrow cells of mice in vivo. Mutagenesis. 2003 Jan 1; 18(1):53-58. https://doi.org/10.1093/mutage/18.1.53. PMid:12473735.

  • Dhouib IE-B, Annabi A, Jrad A, El-Golli N, Gharbi N, Lasram MM, El-Fazaa. Carbosulfan-induced oxidative damage following subchronic exposure and the protective effects of N-acetylcysteine in rats. Gen Physiol Biophys. 2015; 34(03):249-261. https://doi.org/10.4149/gpb_2015005. PMid:25926552.

  • Dhouib, Ines El-Bini. Anti-inflammatory effects of n-acetylcysteine against carbosulfan-induced hepatic impairment in male rats. Recent Adv Biol Med. 2015; 01:29. https://doi.org/10.18639/RABM.2015.01.156935.

  • Kaliwal BB, Ksheerasagar RL. Histological and biochemical changes in the liver of albino mice on exposure to insecticide, carbosulfan. Casp J Environ Sci. 2006; 4(1):67-76.

  • Cui J, Wang F, Gao J, Zhai W, Zhou Z, Liu D, Wang P. Bioaccumulation and metabolism of carbosulfan in Zebrafish (Danio rerio) and the toxic effects of its metabolites. J Agric Food Chem. 2019; 67(45):12348-12356. https://doi.org/10.1021/acs.jafc.9b03674. PMid:31638788.

  • Nwani CD, Lakra WS, Nagpure NS, Kumar R, Kushwaha B, Srivastava SK. Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food Chem Toxicol. 2010; 48(1):202-208. https://doi.org/10.1016/j.fct.2009.09.041. PMid:19818828.

  • Abdelhamid FM, Mahgoub HA, Ateya AI. Ameliorative effect of curcumin against lead acetate-induced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats. Environ Sci Pollut Res. 2020; 27(10):10950-10965. https://doi.org/10.1007/s11356-020-07718-3. PMid:31953765.

  • Farghali H, Canová NK, Zakhari S. Hepatoprotective properties of extensively studied medicinal plant active constituents: possible common mechanisms. Pharm Biol. 2015; 53(6):781-791. https://doi.org/10.1007/s11517-015-1286-7. PMid:25850981.

  • Jaiswal SK, Gupta VK, Siddiqi NJ, Pandey RS, Sharma B. Hepatoprotective effect of citrus limon fruit extract against carbofuran induced toxicity in Wistar rats. Chin J Biol. 2015; 2015:1-10. https://doi.org/10.1155/2015/686071.

  • Jainu M, Devi CSS. In vitro and In vivo evaluation of free-radical scavenging potential of Cissus quadrangularis. Pharm Biol. 2005; 43(9):773-779. https://doi.org/10.1080/13880200500406636.

  • Lee HJ, Le B, Lee D-R, Choi B-K, Yang SH. Cissus quadrangularis extract (CQR-300) inhibits lipid accumulation by down regulating adipogenesis and lipogenesis in 3T3-L1 cells. Toxicol Rep. 2018; 5:608-614. https://doi.org/10.1016/j.toxrep.2018.02.008. PMid:29854631 PMCid:PMC5977379.

  • Nash R, Azantsa B, Kuate D, Singh H, Oben J. The Use of a Stem and leaf aqueous extract of Cissus quadrangularis (CQR-300) to reduce body fat and other components of metabolic syndrome in overweight participants. J Altern Complement Med. 2018; 25(1):98-106. https://doi.org/10.1089/acm.2018.0016. PMid:29912570 PMCid:PMC6352552.

  • Sawangjit R, Puttarak P, Saokaew S, Chaiyakunapruk N. Efficacy and safety of Cissus quadrangularis L. in clinical use: A systematic review and meta-analysis of randomized controlled trials. Phytother Res. 2017; 31(4):555-567. https://doi.org/10.1002/ptr.5783. PMid:28165166.

  • Zenebe S, Feyera T, Assefa S. In vitro anthelmintic activity of crude extracts of aerial parts of Cissus quadrangularis L. and leaves of Schinus molle L. against Haemonchus contortus. Bio Med Research International. 2017; 2017:6. https://doi.org/10.1155/2017/1905987. PMid:29410960 PMCid:PMC5749317.

  • Kokilavani P, Suriyakalaa U, Elumalai P, Abirami B, Ramachandran R, Sankarganesh A, et al. Antioxidant mediated ameliorative steroidogenesis by Commelina benghalensis L. and Cissus quadrangularis L. against quinalphos induced male reproductive toxicity. Pestic Biochem Phys. 2014; 109:18-33. https://doi.org/10.1016/j.pestbp.2014.01.002. PMid:24581381.

  • Lekshmi RK, Sreekutty MS, Mini S. The regulatory effects of Cissus quadrangularis on some enzymes involved in carbohydrate metabolism in streptozotocin-induced diabetic rats. Pharm Biol. 2015; 53(8):1194-1200. https://doi.org/10.3109/13880209.2014.970286. PMid:25857823.

  • Lekshmi RK, Rajesh R, Mini S. Ethyl acetate fraction of Cissus quadrangularis stem ameliorates hyperglycaemia-mediated oxidative stress and suppresses inflammatory response in nicotinamide/streptozotocin induced type 2 diabetic rats. Phytomedicine. 2015; 22(10):952-960. https://doi.org/10.1016/j.phymed.2015.06.014. PMid:26321745.

  • Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure (En) OECD (Internet). 2021. Cited 2021 May 15. Available from: https://www.oecd.org/env/test-no-425-acute-oral-toxicity-up-and-down-procedure-9789264071049-en.htm.

  • Bancroft JD. Theory and Practice of Histological Techniques. Elsevier Health Sciences; 2008.

  • Hood RD, Hood RD. Developmental and Reproductive Toxicology: A Practical Approach. CRC Press; 2005. https://doi.org/10.1201/9781420040548.

  • World Health Organization, Editor. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th Ed. Geneva: World Health Organization; 2010.

  • Banji D, Banji OJF, Ragini M, Annamalai AR. Carbosulfan exposure during embryonic period can cause developmental disability in rats. Environ Toxicol and Pharmacol. 2014; 38(1):230-238. https://doi.org/10.1016/j.etap.2014.05.009. PMid:24973665.

  • Dirican EK, Kalender Y. Dichlorvos-induced testicular toxicity in male rats and the protective role of vitamins C and E. Exp Toxicol Pathol. 2012; 64(7):821-830. https://doi.org/10.1016/j.etp.2011.03.002. PMid:21458248.

  • Ksheerasagar RL, Kaliwal BB. Temporal effects of carbosulfan on testicular biochemical parameters and enzyme activities in albino mice. Int J Pharm Bio Sci (B). 2013; 4:173-181.

  • Alaa-Eldin EA, El-Shafei DA, Abouhashem NS. Individual and combined effect of chlorpyrifos and cypermethrin on reproductive system of adult male albino rats. Environ Sci Pollut Res. 2017; 24(2):1532-1543. https://doi.org/10.1007/s11356-016-7912-6. PMid:27785720.

  • Abdellatief RB, Elgamal DA, Mohamed EEM. Effects of chronic tramadol administration on testicular tissue in rats: an experimental study. Andrologia. 2015; 47(6):674-679. https://doi.org/10.1111/and.12316. PMid:25228095.

  • Hayes FJ, DeCruz S, Seminara SB, Boepple PA, Crowley Jr WF. Differential regulation of gonadotropin secretion by testosterone in the human male: Absence of a negative feedback effect of testosterone on follicle-stimulating hormone secretion. J Clin Endocrinol Metab. 2001; 86(1):53-58. https://doi.org/10.1210/jcem.86.1.7101. PMid:11231978.

  • Patrick SM, Bornman MS, Joubert AM, Pitts N, Naidoo V, de Jager C. Effects of environmental endocrine disruptors, including insecticides used for malaria vector control on reproductive parameters of male rats. Reprod Toxicol. 2016; 61:19-27. https://doi.org/10.1016/j.reprotox.2016.02.015. PMid:26928317.

  • Hassan HF, Meligi NM. Effects of sublethal Abamectin exposure on some hormonal profiles and testicular histopathology in male albino rats and the possible ameliorative role of Eruca sativa. Environ Sci Pollut Res. 2017; 24(31):24690-24697. https://doi.org/10.1007/s11356-017-0112-1. PMid:28913779.

  • Akinwande DV, Adeyemi JA, Olawuyi ST, Akinola BK, Adedire CO. Protective effects of Camellia sinensis on Syzygium aromaticum or chlorpyrifos-induced reproductive toxicity in male Wistar rats. J Basic Appl Zool. 2019; 80(1):48. https://doi.org/10.1186/s41936-019-0122-2.

  • Tetsatsi ACM, Nkeng-Effouet PA, Alumeti DM, Bonsou GRF, Kamanyi A, Watcho P. Colibri® insecticide induces male reproductive toxicity: Alleviating effects of Lannea acida (Anacardiaceae) in rats. Basic Clin Androl. 2019; 29(1):16. https://doi.org/10.1186/s12610-019-0096-4. PMid:31890217 PMCid:PMC6924042.

  • Saber TM, Abd El-Aziz RM, Ali HA. Quercetin mitigates fenitrothion-induced testicular toxicity in rats. Andrologia. 2016; 48(5):491-500. https://doi.org/10.1111/and.12467. PMid:26264430.

  • Costa NO, Vieira ML, Sgarioni V, Pereira MRF, Montagnini BG, Mesquita S de FP, Gerardin DC. Evaluation of the reproductive toxicity of fungicide propiconazole in male rats. Toxicology. 2015; 335:55-61. https://doi.org/10.1016/j.tox.2015.06.011. PMid:26169826.

  • Taşdemir I, Taşdemir M, Tavukçuoglu S, Kahraman S, Biberoģlu K. Effect of abnormal sperm head morphology on the outcome of intracytoplasmic sperm injection in humans. Hum Reprod. 1997; 12(6):1214-1217. https://doi.org/10.1093/humrep/12.6.1214. PMid:9222003.

  • Wyrobek AJ, Bruce WR. Chemical induction of sperm abnormalities in mice. Proc Nat Acad Sci. 1975; 72(11):4425-4429. https://doi.org/10.1073/pnas.72.11.4425. PMid:1060122 PMCid:PMC388734.

  • Khan S, Jan MH, Kumar D, Telang AG. Firpronil induced spermotoxicity is associated with oxidative stress, DNA damage and apoptosis in male rats. Pestic Biochem Phys. 2015; 124:8-14. https://doi.org/10.1016/j.pestbp.2015.03.010. PMid:26453224.

  • Ben Slima A, Chtourou Y, Barkallah M, Fetoui H, Boudawara T, Gdoura R. Endocrine disrupting potential and reproductive dysfunction in male mice exposed to deltamethrin. Hum Exp Toxicol. 2017; 36(3):218-226. https://doi.org/10.1177/0960327116646617. PMid:27164926.

  • Sarbishegi M, Alhagh Charkhat Gorgich E, Khajavi O. Olive leaves extract improved sperm quality and antioxidant status in the testis of rat exposed to rotenone. NephroUrol Mon. 2017; 9(3):1-7. https://doi.org/10.5812/numonthly.47127.

  • Mosbah R, Djerrou Z, Mantovani A. Protective effect of Nigella sativa oil against acetamiprid induced reproductive toxicity in male rats. Drug Chem Toxicol. 2018; 41(2):206-212. https://doi.org/10.1080/01480545.2017.1337127. PMid:28669218.

  • Nahid Z, Tavakol HS, Abolfazl GK, Leila M, Negar M, Hamed F, et al. Protective role of green tea on malathion-induced testicular oxidative damage in rats. Asian Pac J Reprod. 2016; 5(1):42-45. https://doi.org/10.1016/j.apjr.2015.12.007.


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  • Carbosulfan Induced Reproductive Toxicity in Male Albino Rats (Rattus Norvegicus) and Protective Effect of Cissus Quadrangularis

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Authors

Lokeshkumar Prakash
Department of Zoology, Karnatak University, Dharwad – 580003, Karnataka,, India
Muniswamy David
Department of Zoology, Karnatak University, Dharwad – 580003, Karnataka,, India

Abstract


Carbosulfan is the most commonly used carbamate insecticide in the agriculture industry and is known to cause diverse toxicological effects on non-target organisms. Farmers are more susceptible to accidental consumption of insecticides owing to their unscientific use and ease of access. The present investigation was carried out to evaluate reproductive toxicity induced by carbosulfan and its amelioration using traditional Cissus quadrangularis plant extract. The rats were divided into six groups; (E1) control treated with 1% DMSO/kg bw, (E2) plant extract alone (1000 mg/kg bw), (E3) low dose of carbosulfan (3.4 mg/kg bw), (E4) high dose of carbosulfan (6.37 mg/kg bw), (E5) low dose (3.4 mg/kg bw) and (E6) high dose (6.37 mg/kg bw) carbosulfan co-treatment with Cissus quadrangularis extract (1000 mg/kg bw) for 60 days. The reproductive parameters including histology of testes, sperm count, motility, morphology, and hormonal assay (FSH, T, LH) were performed. Carbosulfan treated groups (E3 & E4) were manifested histopathological alterations including tubular degeneration, disorganisation of cells, vacuolisation, and sloughing of germ cells into the tubular lumen. The hormonal level, sperm count, and motility were significantly decreased and the sperm abnormality rate was increased in the E4 group compared to the control group. The groups (E5 & E6) co-treated with plant extract were significantly recovered compared to insecticide-treated groups (E3 & E4). The histological and hormonal changes were attributed to the reprotoxic effects of the insecticide. However, co-treatment of plant extract significantly recovered the toxic effects caused by low concentration (E5) than the high concentration (E6) of carbosulfan.

Keywords


Carbosulfan, Cissus quadrangularis, Plant Extract, Reproductive Toxicity, Testes Histology

References





DOI: https://doi.org/10.18311/ti%2F2022%2Fv29i1%2F28635