Toxicology International (Formerly Indian Journal of Toxicology)

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Toxic Effect of Silver Nanoparticles on Liver, Gill and Muscle Tissues of Zebrafish Danio rerio


Affiliations
1 Department of Zoology, B. P. Arts, Science and Commerce College, Chalisgaon, KBC, North Maharashtra University, Jalgaon – 424101, Maharashtra, India
     

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The present study was aimed to evaluate the toxic effect of Silver nanoparticle on the liver, gill and muscle tissues of the Zebrafish, Danio rerio.  The experiment was designed to understand the chronic toxicity of silver nanoparticles (AgNPs) in adult fish, Danio rerio.  In the chronic toxicity study adult fish, Danio rerio were divided in to two groups.  First group was experimental group in which fish were successively treated with a graded series of 0.3, 0.6, 0.9 mg/l an average 60 nm. PVP. coated AgNPs treatment were given for 21 days, at the end of experimental period, Reduced glutathione activity (GSH), Lipid peroxidation activity (LPO), Lactate dehydrogenase (LDH) and Total protein  in liver, gill and muscle tissues were assayed. Second group were kept as a control which was free from AgNPs exposure.  The levels of Reduced glutathione activity (GSH) and Total protein were found to be decreased were as Lipid peroxidation activity (LPO) and Lactate dehydrogenase activity (LDH) were found to be elevated in liver, gill and muscle tissue of AgNPs treated Zebrafish Danio rerio.

Keywords

Danio rerio, Nanotoxicology, Silver Nanoparticles (AgNPs).
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  • Xia G, Liu T, Wanh Z, Hou Y, Dong L. The effect of silver nanoparticles on embryonic development and toxicology. Artif Cells Nanomed Biotechnol. 2016; 44:1116–21.

  • Bone AJ, Colman BP, Gondikas AP, Newton KM, Harrold KH. Biotic and abiotic connections in sea-going microcosms decide destiny and poisonousness of Ag nanoparticles: Part 2-harmfulness and Ag speciation. Environ Sci Technol. 2012; 46:6925–33. PMid: 22680837. https://doi.org/10.1021/es204683m

  • Ivask K, Juganson O, Bondarenko M, Mortimer V, Aruoja, Kasemets K. Systems of poisonous activity of Ag, ZnO and CuO nanoparticles to choose ecotoxicological test organic entities and mammalian cells in vitro: A comparative review. Nanotoxicology. 2014; 8:57–71. PMid: 24256211. https://doi.org/10.3109/17435390.2013.855831

  • Duran N, Duran M, Jesus MBD, Seabra AB, Favaro WJ, Nakazato G. Silver nanoparticles: A new view on mechanistic aspects on antimicrobial movement. NanomedNanotechnol Biol Med. 2016; 12:789–99. PMid: 26724539. https://doi.org/10.1016/j.nano.2015.11.016

  • Ale A, Rossi AS, Bacchetta C. Integrative assessment of silver nanoparticles toxicity in in Prochilodus lineatus fish. Ecol Indic. 2018; 93:1190–8. https://doi.org/10.1016/j.ecolind.2018.06.023

  • Cameron SJ, Hosseinian F, Willmore WG. A current overview of the biological and cellular effects of nanosilver. Int J Mol Sci. 2018; 19:30–40. PMid: 30002330 PMCid: PMC6073671. https://doi.org/10.3390/ijms19072030

  • Speshock JL, Elrod N, Sadoski DK, Maurer E, Braydich-Stolle LK. Differential organ toxicity in the adult zebrafish following openness to acute sub-deadly doses of 10 nm silver nanoparticles. Front Nanosci Nanotech, 2016; 2:114–20. https://doi.org/10.15761/FNN.1000119

  • Devi GP, Ahmed KB, Varsha MK, Shrijha BS, Lal KK. Sulfidation of silver nanoparticle reduces its toxicity in zebrafish. Aquat Toxicol. 2015; 158:149–56. PMid: 25438120. https://doi.org/10.1016/j.aquatox.2014.11.007

  • OECD, (Organization for Economic Co-activity and Development) Guideline for the Testing of Chemicals: Fish, acute toxicity test. Document 203, 1992; OECD, Paris, France.

  • Henglein A. Colloidal silver nanoparticles: Photochemical arrangement and cooperation with O2 , CCl4 and some metal ions. Chem Mater. 1998; 10:444–50. https://doi.org/10.1021/cm970613j

  • Bacchetta C, Rossi A, Ale A, Campana M, Parma MJ, Cazenave J. Combined toxicological effects of pesticides: A fish multi-biomarker approach. Ecol Indic. 2014; 36:532–8. https://doi.org/10.1016/j.ecolind.2013.09.016

  • Boyne AF, Ellman GL. A methodology for analysis of tissue sulfhydryl components. Anal Biochem. 1972; 46(2):639–53. PMid: 4623507. https://doi.org/10.1016/0003-2697(72)90335-1

  • Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302–10. PMid: 672633. https://doi.org/10.1016/S0076-6879(78)52032-6

  • King J. The dehydrogenase of oxido reductase lactate dehydrogenase. Reasonable Clinical Enzymology (Ed. D .Van), Nostrand Co., London, 1965; pp. 83–93.

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193:265. PMid: 14907713. https://doi.org/10.1016/S0021-9258(19)52451-6

  • Chae YJ, Pham CH, Lee J, Bae E, Yi J, Gu MB. Evaluation of the toxic impact of silver nanoparticles on Japanese medaka Oryziaslatipes. Aquat Toxicol. 2009; 94:320–7. PMid: 19699002. https://doi.org/10.1016/j.aquatox.2009.07.019

  • Pena-Llopis S, Ferrando MD, Pena JB. Increased recovery of brain acetylcholinesterase activity in dichlorvos-inebriated European eels Anguilla anguilla by bath treatment with N-acetylcysteine. Dis Aquat Organ. 2003; 55:237–45. PMid: 13677510. https://doi.org/10.3354/dao055237

  • Trivedi MH, Sangai NP, Renuka A. Assessment of toxicity of copper sulfate pentahydrate on oxidative stress indicators on liver of gold fish, Carassius auratus Bull. Environ Pharmacol Life Sci. 2012; 1(9):52–7.

  • Valerio-Garciaa RC, Carbajal-Hernandeza AL, Martinez-Ruiza EB, Jarquin-Diaza VHC, Haro-Perezb F, Martinez-Jeronim. Exposure to silver nanoparticles produces oxidative pressure and affects macromolecular and metabolic biomarkers in the goodeid fish, Chapal ichthy spardalis. Sci All out Environ. 2017; 583:308–18. PMid: 28117161. https://doi.org/10.1016/j.scitotenv.2017.01.070

  • Abdel-Hameid NA. Physiological and histopathological alterations induced by phenol exposure in Oreochromis aureus Juveniles. Tur J Fish Aqua Sci. 2007; 7:131–8.

  • Atli G, Ariyurek SY, Kanak EG, Canli M. Alterations in the serum biomarkers belonging to different metabolic systems of fish, Oreochromis niloticus after Cd and Pb openings. Environ Toxicol Pharmacol. 2015; 40:508–15. PMid: 26310509. https://doi.org/10.1016/j.etap.2015.08.001

  • Ramesh M, Anitha S, Poopal RK, Shobana C. Evaluation of acute and sublethal impacts of chloroquine (C18H26CIN3) on certain enzymological and histopathological biomarker reactions of a freshwater fish Cyprinus carpio. Toxicol. 2018, 5:18–27. PMid: 29270363 PMCid: PMC5734797. https://doi.org/10.1016/j.toxrep.2017.11.006

  • Vignardi CP, Hasue FM, Sartorio PV, Cardoso CM, Maghado AS, Passos MJ, Santos TC, Nucci JM, Hewer TL, Watanabe IS and Gomes V. Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766). Aquat. Toxicol, 2015; 158: 218-229. https://doi.org/10.1016/j.aquatox.2014.11.008.

  • Rendon-Von Osten J, Ortiz-Arana A, Guilhermino L, Soares AM. In vivo evaluation of three biomarkers in the mosquito fish, Gambusia yucatana presented to pesticides. Chemosphere. 2005; 58(5):627–36. PMid: 15620756. https://doi.org/10.1016/j.chemosphere.2004.08.065

  • Kong X, Wang G, Li S. Impacts of low temperature acclimation on antioxidant defenses and ATPase practices in the muscle of mud crab Scylla paramamosain. Aquaculture. 2012; 370-371:144–9. https://doi.org/10.1016/j.aquaculture.2012.10.012

  • Alkaladi A, Nasr AM, El-Deen NM, Afifi OA, Abu Zinadah. Hematological and biochemical investigations on the impact of vitamin E and C on Oreochromis niloticus presented to zinc oxide nanoparticles Saudi. J Biol Sci. 2015; 22:556–63. PMid: 26288558 PMCid: PMC4537867. https://doi.org/10.1016/j.sjbs.2015.02.012

  • Qin Q, Qin S, Wang L, Lei W. Immune response and ultrastructural changes of hemocytes in freshwater crab Sinopotamonhenanense presented to raise cadmium. Aquat Toxicol. 2012; 106-107:140–6. PMid: 22155426. https://doi.org/10.1016/j.aquatox.2011.08.013

  • Hermes-Lima M. Oxygen in biology and biochemistry: Role of free extremists Ed. Story, K.B. Utilitarian Metabolism: Regulation Adaptation. John Wiley and Sons, Inc., Hoboken, NJ, USA. 2005; Pp. 319–68. https://doi.org/10.1002/047167558X.ch12

  • Vogel C, Silva GM, Marcotte EM. Protein expression regulation under oxidative stress. Mol Cell Proteomics. 2011; 10:M111.009217. PMid: 21933953 PMCid: PMC3237073. https://doi.org/10.1074/mcp.M111.009217


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  • Toxic Effect of Silver Nanoparticles on Liver, Gill and Muscle Tissues of Zebrafish Danio rerio

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Authors

A. D. Shelke
Department of Zoology, B. P. Arts, Science and Commerce College, Chalisgaon, KBC, North Maharashtra University, Jalgaon – 424101, Maharashtra, India

Abstract


The present study was aimed to evaluate the toxic effect of Silver nanoparticle on the liver, gill and muscle tissues of the Zebrafish, Danio rerio.  The experiment was designed to understand the chronic toxicity of silver nanoparticles (AgNPs) in adult fish, Danio rerio.  In the chronic toxicity study adult fish, Danio rerio were divided in to two groups.  First group was experimental group in which fish were successively treated with a graded series of 0.3, 0.6, 0.9 mg/l an average 60 nm. PVP. coated AgNPs treatment were given for 21 days, at the end of experimental period, Reduced glutathione activity (GSH), Lipid peroxidation activity (LPO), Lactate dehydrogenase (LDH) and Total protein  in liver, gill and muscle tissues were assayed. Second group were kept as a control which was free from AgNPs exposure.  The levels of Reduced glutathione activity (GSH) and Total protein were found to be decreased were as Lipid peroxidation activity (LPO) and Lactate dehydrogenase activity (LDH) were found to be elevated in liver, gill and muscle tissue of AgNPs treated Zebrafish Danio rerio.

Keywords


Danio rerio, Nanotoxicology, Silver Nanoparticles (AgNPs).

References