Toxicology International (Formerly Indian Journal of Toxicology)

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Toxic Effect of Silver Nanoparticles on Liver, Gill and Muscle Tissues of Zebrafish Danio rerio


Affiliations
1 Department of Zoology, B. P. Arts, Science and Commerce College, Chalisgaon, KBC, North Maharashtra University, Jalgaon – 424101, Maharashtra, India
     

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The present study was aimed to evaluate the toxic effect of Silver nanoparticle on the liver, gill and muscle tissues of the Zebrafish, Danio rerio.  The experiment was designed to understand the chronic toxicity of silver nanoparticles (AgNPs) in adult fish, Danio rerio.  In the chronic toxicity study adult fish, Danio rerio were divided in to two groups.  First group was experimental group in which fish were successively treated with a graded series of 0.3, 0.6, 0.9 mg/l an average 60 nm. PVP. coated AgNPs treatment were given for 21 days, at the end of experimental period, Reduced glutathione activity (GSH), Lipid peroxidation activity (LPO), Lactate dehydrogenase (LDH) and Total protein  in liver, gill and muscle tissues were assayed. Second group were kept as a control which was free from AgNPs exposure.  The levels of Reduced glutathione activity (GSH) and Total protein were found to be decreased were as Lipid peroxidation activity (LPO) and Lactate dehydrogenase activity (LDH) were found to be elevated in liver, gill and muscle tissue of AgNPs treated Zebrafish Danio rerio.

Keywords

Danio rerio, Nanotoxicology, Silver Nanoparticles (AgNPs).
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  • Toxic Effect of Silver Nanoparticles on Liver, Gill and Muscle Tissues of Zebrafish Danio rerio

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Authors

A. D. Shelke
Department of Zoology, B. P. Arts, Science and Commerce College, Chalisgaon, KBC, North Maharashtra University, Jalgaon – 424101, Maharashtra, India

Abstract


The present study was aimed to evaluate the toxic effect of Silver nanoparticle on the liver, gill and muscle tissues of the Zebrafish, Danio rerio.  The experiment was designed to understand the chronic toxicity of silver nanoparticles (AgNPs) in adult fish, Danio rerio.  In the chronic toxicity study adult fish, Danio rerio were divided in to two groups.  First group was experimental group in which fish were successively treated with a graded series of 0.3, 0.6, 0.9 mg/l an average 60 nm. PVP. coated AgNPs treatment were given for 21 days, at the end of experimental period, Reduced glutathione activity (GSH), Lipid peroxidation activity (LPO), Lactate dehydrogenase (LDH) and Total protein  in liver, gill and muscle tissues were assayed. Second group were kept as a control which was free from AgNPs exposure.  The levels of Reduced glutathione activity (GSH) and Total protein were found to be decreased were as Lipid peroxidation activity (LPO) and Lactate dehydrogenase activity (LDH) were found to be elevated in liver, gill and muscle tissue of AgNPs treated Zebrafish Danio rerio.

Keywords


Danio rerio, Nanotoxicology, Silver Nanoparticles (AgNPs).

References