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Vahalia, Mahesh K.
- Genotoxic and Mutagenic Activity of Suvarna Bhasma
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Authors
Nilakash Selkar
1,
Sharad Bhagat
1,
Mukesh Chawada
1,
Mahesh K. Vahalia
1,
Anand Puranik
1,
Geeta Vanage
1
Affiliations
1 National Centre for Preclinical Reproductive and Genetic Toxicology National Institute for Research in Reproductive Health, Mumbai, IN
1 National Centre for Preclinical Reproductive and Genetic Toxicology National Institute for Research in Reproductive Health, Mumbai, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 23, No 3 (2016), Pagination: 221-228Abstract
Genetic toxicity and mutagenicity of Suvarna Bhasma (SB), an Ayurvedic drug, was determined using a battery of tests. The results of In-vivo Micronucleus assay and COMET assay did not reveal any significant increase in % Micronucleus frequency(MN) in bone marrow cells of mice and DNA damage in blood lymphocytes respectively after the oral administration of SB at various concentrations (3,-30 mg/kg bw) in treated animals as compared to vehicle control in either sex. The invitro chromosome aberration (CA) assay carried out with and without metabolic activation at different concentrations of SB in human lymphocyte culture did not cause any effect on structural or numerical chromosome aberrations. Suvarna Bhasma did not induce any mutagenic activity in presence and absence of S9 fractions in Ames assay employing three strains of salmonella typhimurium TA98, TA100 and TA102.These results demonstrated that Suvarna Bhasma preparation evaluated in this study is not genotoxic and mutagenic at the concentrations tested under the experimental conditions.Keywords
Suvarna Bhasma, Genotoxicity, Mutagenicity, Micronucleus Assay, COMET Assay, Chromosome Aberration Assay, Ames Assay.
References
- Svarna Bhasma. in: Bharat BhaishyajyaRatnakar,Khand 5 New Delhi,B. Jain Publishers, 1999Yoga No.8357.
- WHO. Legal status of traditional medicine, complementary/ alternativemedicine: a worldwide review. World Health Organization, Geneva; 2001: 1-199.
- WHO. Traditional Medicine Strategy 2002–2005. World Health Organization,Geneva.;2002.
- Raghunathan K. in:Pharmacopeial Standards for Ayurvedic Formulations. New Delhi India: Central Council for Research in Indian Medicine Homeopathy.;1976 :15
- Bajaj S, Vohora SB. Anti-Cataleptic, Anti-Anxiety and AntiDepressant Activity of Gold Preparations Used in Indian Systems of Medicine. Ind J Pharmacol. 2000;32:339–346.
- Shah ZA, Vohora SB. Antioxidant/Restorative Effects of Calcined Gold Preparations Used in Indian Systems of Medicine against Global and Focal Models of Ischaemia. Pharmacol Toxicol. 2002;90:254–259.
- Shah ZA, Gilani RA, Sharma P, Vohora SB. Attenuation of Stress-Elicited Brain Catecholamines, Serotonin and Plasma Corticosterone Levels by Calcined Gold Preparations Used in Indian System of Medicine. Basic Clin Pharmacol Toxicol. 2005;96:469–474.
- Ghosh P, Han G, De M, Kim CK, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev. 2008;60:1307–15.
- Mitra A, Chakraborty S, Auddy B, Tripathi T, Sen S, Saha AV, et al. Evaluation of chemical constituents and free-radical scavenging activity of Swarnabhasma (gold ash), an Ayurvedic drug. J Ethnopharmacol. 2002;80:147–153.
- SinghNP, McCoy MT,TiceRR et al. A simple technique for quantitationof low levels of DNA damage in individual cells.Exp. Cell Res. 1988;175:184–191.
- Maron DM, Ames BN. Revised methods for the Salmonellamutagenicity test. Mut Res. 1983;113: 173-215.
- OECD guideline for the testing of chemicals, OECD guideline no. 473.;1997.
- OECD. Guideline for the testing of chemicals, OECD guideline no. 474.;1997:18/34/1948442.
- Cavalcanti BC, Ferreiraa, JR, Moura DJ et al.. Structure-mutagenicityrelationship of kaurenoic acid from Xylopiasericeae (Annonaceae). Mut Res. 2010;701:153–163.
- Ogura R, Ikeda N, Yuki K, Morita O et al. Genotoxicity studies on green tea catechin. Food and Chem Toxicol. 2008;46: 2190–2200.
- Wang J, Yu S, Jiao S et al. Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells. Mut Res.2012;1:16–23.
- Dash VB, Kashyap L. Processing of Metals and Minerals. in:Iotra-Chemistry of Ayurveda (Rasa Sastra) New Delhi: Concept Publishing Company;1994;20:234–308.