Asian Journal of Chemistry and Pharmaceutical Sciences

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Study on Lipid Content and Fatty Acid Profile of Four Marine Macro Algae (Seaweeds) Collected from South East Coast of Sri Lanka


Affiliations
1 Analytical Chemistry Laboratory, National Aquatic Resources Research and Development Agency, Crow Island, Colombo - 15, Sri Lanka
2 Department of Chemistry, Faculty of Applied Science, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
 

The lipid content and fatty acid profile of four marine macro algae (Ulva lactuca, Sargussum wightii, Sargussum turbinaria, and Kappaphycus alvarezii) that are collected from south east coast of Sri Lanka were studied by solvent extraction followed by gas chromatographic analysis. The results indicated that, the saturated and unsaturated fatty acids levels from C4:0 to C22:6. The lipid content of the selected seaweeds varied from 0.49-1.51% and the highest lipid content was found in U. lactuca while the lowest lipid content was found in the S. turbinaria. The analysis of differences in the composition of the saturated to unsaturated fatty acids in the extracts, shown that, palmitic acid (C16:0) and linoleic acid (C18:2 cis 9, 12) reached the highest value in all selected seaweeds and the highest quantity of monounsaturated fatty acids was observed in S. wightii (18.35%) followed by S. turbinaria (15.32%) and K. alvarezii (15.20%). An omega-3 fatty acid such as, alpha linolenic acid (all-cis-6, 9, 12), linolenic acid (all-cis-9, 12, 15), and eicosatrienoic acid (all-cis-8, 11, 14) were rich in U. lactuca, S. wightii and S. turbinaria. But some seaweeds have been detected the butyric acid (C4:0) in higher proportion (5.58-28.94%) and it may due to the fermentation of the seaweeds. The obtained results helped to determine that selected seaweeds have higher content of saturated fatty acids than the unsaturated fatty acids and proper drying condition and storage condition will be need to the seaweeds.


Keywords

Butyric Acid, Fatty Acids, Palmitic Acid, Saturated Fatty Acids, Unsaturated Fatty Acids.
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  • Kaliaperumal N. Products from seaweeds SDMRI research publication; 2003. p. 10.

  • Polat S, Ozogul Y. Fatty acid, mineral and proximate composition of some seaweeds from the northeastern Mediterranean coast. Italian Journal of Food Science. 2009; 21(3):317–24.

  • Kumar M, Kumari P, Trivedi N, Shukla MK, Gupta V, Reddy C, et al. Minerals, PUFAs and antioxidant properties of some tropical seaweeds from Saurashtra coast of India. Journal of Applied Phycology. 2011; 23(5):797–810. https://doi.org/10.1007/s10811-010-9578-7

  • Zhang H, Wang Z, Liu O. Development and validation of a GC-FID method for quantitative analysis of oleic acid and related fatty acids. Journal of Pharmaceutical Analysis. 2015; 5(4):223–30. https://doi.org/10.1016/j.jpha.2015.01.005 PMid:29403935 PMCid:PMC5762211

  • Sukhija PS, Palmquist D. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry. 1988; 36(6):1202–6. https://doi.org/10.1021/jf00084a019

  • Rodriguez-Ruiz J, Belarbi E-H, Sanchez JLG, Alonso DL. Rapid simultaneous lipid extraction and transesterification for fatty acid analyses. Biotechnology Techniques. 1998; 12(9):689–91. https://doi.org/10.1023/A:1008812904017

  • Khotimchenko S. Fatty acids of brown algae from the Russian Far East. Phytochemistry. 1998; 49:2363–9. https:// doi.org/10.1016/S0031-9422(98)00240-4

  • Ivanova V, Stancheva M, Petrova D. Fatty acid composition of black sea Ulva rigida and Cystoseira crinita. Bulg J Agric Sci. 2013; 19(Suppl 1):42–7.

  • Simopoulos AP. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Experimental biology and medicine. 2008; 233(6):674–88. https://doi.org/10.3181/0711-MR-311 PMid:18408140

  • Hossain AS, Salleh A, Boyce AN, Chowdhury P, Naqiuddin M. Biodiesel fuel production from algae as renewable energy. American Journal of Biochemistry and Biotechnology. 2008; 4(3):250–4. https://doi.org/10.3844/ajbbsp.2008.250.254

  • Manivannan K, Thirumaran G, Karthikai G, Anantharaman P, Balasubramanian T. Proximate composition of different group of seaweeds from vedalai coastal waters (Gulf of Mannar): Southeast coast of India. Middle-East Journal of Scientific Research. 2009; 4(2):72–7.

  • Sudhakar M, Manivannan K, Soundrapandian P. Nutritive value of hard and soft shell crabs of Portunus sanguinolentus (Herbst). Journal Animal and Veterinary Advances. 2009; 1(2):44–8.

  • Ahamad F, Sulaiman MR, Saiman W, Yee CF, Matanjun P. Proximate composition and total penolic contents of the selected edible seaweed from semporna, Sabah, Malayasia. Borneo Science. 2012; 31:12.

  • Wong KH, Cheung, Peter CK. Nutritional evaluation of some subtropical red and green seaweeds: Part I—proximate composition, amino acid profiles and some physicochemical properties. Food Chemistry. 2000; 71(4):475–82. https://doi.org/10.1016/S0308-8146(00)00175-8

  • Syad AN, Shunmugiah KP, Kasi PD. Seaweeds as nutritional supplements: Analysis of nutritional profile, physicochemical properties and proximate composition of G. acerosa and S. wightii. Biomedicine and Preventive Nutrition. 2013; 3(2):139–44. https://doi.org/10.1016/j.bionut.2012.12.002

  • Otleş S, Pire R. Fatty acid composition of Chlorella and Spirulina microalgae species. Journal of AOAC International. 2001; 84(6):1708–14. PMid:11767135

  • Chan JC-C, Cheung PC-K, Ang PO. Comparative studies on the effect of three drying methods on the nutritional composition of seaweed Sargassum hemiphyllum (Turn.) C. Ag. Journal of Agricultural and Food Chemistry. 1997; 45(8):3056–9. https://doi.org/10.1021/jf9701749

  • El-Kassas HY. Growth and fatty acid profile of the marine microalga Picochlorum sp grown under nutrient stress conditions. The Egyptian Journal of Aquatic Research. 2013; 39(4):233–9. https://doi.org/10.1016/j.ejar.2013.12.007

  • Huesemann MH, Kuo L-J, Urquhart L, Gill GA, Roesijadi G. Acetone-butanol fermentation of marine macroalgae. Bioresource Technology. 2012; 108:305–9. https://doi.org/10.1016/j.biortech.2011.12.148 PMid:22277213

  • Norziah MH, Ching CY. Nutritional composition of edible seaweed Gracilaria changgi. Food Chemistry. 2000; 68(1):69–76. https://doi.org/10.1016/S03088146(99)00161-2

  • Muralidhar AP, Syamala K, Prakash C, Kalidas C, Prasad NR. Comparative studies on fatty acid composition of three marine macroalgae collected from Mandapam region : South East Coast of India. World Applied Sciences Journal. 2010; 11(8):958–65.

  • Li X, Fan X, LHan, Lou Q. Fatty acids of some algae from the Bohai Sea. Phytochemistry. 2002; 59:157–61. https:// doi.org/10.1016/S0031-9422(01)00437-X

  • Sanchez-Machado D, Lopez-Cervantes J, Lopez-Hernandez J, Paseiro-Losada P. Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chemistry. 2004; 85(3):439–44. https://doi.org/10.1016/j.foodchem.2003.08.001

  • Simopoulos AP. Essential fatty acids in health and chronic disease. The American Journal of Clinical Nutrition. 1999; 70(3): 560s–9s. https://doi.org/10.1093/ajcn/70.3.560s PMid:10479232


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  • Study on Lipid Content and Fatty Acid Profile of Four Marine Macro Algae (Seaweeds) Collected from South East Coast of Sri Lanka

Abstract Views: 723  |  PDF Views: 640

Authors

G. D. T. M. Jayasinghe
Analytical Chemistry Laboratory, National Aquatic Resources Research and Development Agency, Crow Island, Colombo - 15, Sri Lanka
B. K. K. K. Jinadasa
Analytical Chemistry Laboratory, National Aquatic Resources Research and Development Agency, Crow Island, Colombo - 15, Sri Lanka
S. D. M. Chinthaka
Department of Chemistry, Faculty of Applied Science, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

Abstract


The lipid content and fatty acid profile of four marine macro algae (Ulva lactuca, Sargussum wightii, Sargussum turbinaria, and Kappaphycus alvarezii) that are collected from south east coast of Sri Lanka were studied by solvent extraction followed by gas chromatographic analysis. The results indicated that, the saturated and unsaturated fatty acids levels from C4:0 to C22:6. The lipid content of the selected seaweeds varied from 0.49-1.51% and the highest lipid content was found in U. lactuca while the lowest lipid content was found in the S. turbinaria. The analysis of differences in the composition of the saturated to unsaturated fatty acids in the extracts, shown that, palmitic acid (C16:0) and linoleic acid (C18:2 cis 9, 12) reached the highest value in all selected seaweeds and the highest quantity of monounsaturated fatty acids was observed in S. wightii (18.35%) followed by S. turbinaria (15.32%) and K. alvarezii (15.20%). An omega-3 fatty acid such as, alpha linolenic acid (all-cis-6, 9, 12), linolenic acid (all-cis-9, 12, 15), and eicosatrienoic acid (all-cis-8, 11, 14) were rich in U. lactuca, S. wightii and S. turbinaria. But some seaweeds have been detected the butyric acid (C4:0) in higher proportion (5.58-28.94%) and it may due to the fermentation of the seaweeds. The obtained results helped to determine that selected seaweeds have higher content of saturated fatty acids than the unsaturated fatty acids and proper drying condition and storage condition will be need to the seaweeds.


Keywords


Butyric Acid, Fatty Acids, Palmitic Acid, Saturated Fatty Acids, Unsaturated Fatty Acids.

References