Asian Journal of Pharmaceutical Research and Health Care

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A simple, rapid, sensitive and precise High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of gatifloxacin in its pharmaceutical dosage forms. In this method RP - C 18 column (150mmx4.6mm I.D., 5 μm particle size) with mobile phase consisting of acetonitrile and 0.05M phosphate buffer in the ratio of 25:75 v/v in isocratic mode was used. The detection wavelength is 293nm and the flow rate is 0.8ml/min. Ciprofloxacin is used as internal standard. The linearity was fo und to be in the range of 0.1 to 10 μg/ml and shows a correlation coefficient of 0.9999. The intra - and inter - day variation was found to be less than 1.2% showing high precision of the assay method. The mean percent recovery of the drug from the solution c ontaining 4 μg/ml was 100.5 ± 2.06 indicating high accuracy of the proposed method. Hence, this method is simple, fast, specific, accurate, precise and less time consuming for the estimation of gatifloxacin in pharmaceutical dosage forms.

Keywords

HPLC, Gatifloxacin, Estimation, Pharmaceutical Dosage Forms.
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