Derivatized HPTLC Method for Simultaneous Estimation of Glucosamine and Ibuprofen in Tablets

A selective, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Glucosamine and Ibuprofen in tablet formulation. In this method standard and sample solutions of Glucosamine and Ibuprofen were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using Propanol-Ethyl acetate-Ammonia-Water (4: 3: 2: 1 v/v), as mobile phase and derivatized using Iodine vapor. The drugs on the plate were scanned at 254 nm. The dynamic linearity range was 5-25 μg/spot for Glucosamine and 2-10 μg/spot for Ibuprofen. The method was validated for precision, recovery and reproducibility.