Screening the Antimicrobial Activity of some Medicinal Plants against Multidrug Resistance Escherichia Coli Type (1)

The increasing number of Escherichia coli causing mastitis and of bacteria resistant to conventional antibiotics has become a serious problem in recent years. So the search for new antibiotics and alternative products to solve this problem is the question of the age. This research aims to investigate the effectiveness of the extracts prepared from different parts of the following plants: Olea europea Linn ( O leaceae ), Myrtus communis Linn (Liliaceae ), Majorana syriacus Linn (Laminaceae ), Zingiber officinale Linn ( Zingiberaceae ), Achillea falcata Linn ( Asteraceae) against resistant Escherichia coli Type (1). Investigation began for E.coli bacteria in 667 milk samples. The bacteria were identified culturally, morphologically and biochemically. Antibiotic susceptibility testing against E.coli by Kirby - Bauer disk diffusion method were conducted. Then using the blood agar, MacConkey agar, salmonella - shigella agar, and biochemical testing method [API 20 E testing Enterobacteriaceae] were made to type E.coli . P lants were extracted with water , absolute alcohol, then either using a soxhlet apparatus and rotary vacuum evaporator. T he n extracts susceptibility testing against antibiotic resistant E.coli Type (1) were studied. E.coli was defined as oxidase negative, indole positive, catalase positive. The studied antibiotics did not show any antibacterial effect against E.coli. By t he results of the biochemical analysis (API20e) on resistant E.coli, E.coli type (1) was 33.35% of the total number of samples. The anti - bacterial effectiveness against E.coli type (1) of ethanol extracts prepared from different parts of the studied plants were variant, whereas the Myrtus communis extract effectively has the most powerful antibacterial effect for these bacteria , while the Zingiber officinale extract has the lowest influence.