Journal of Advances in Engineering Sciences

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Fermentative Synthesis of Lactic Acid


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1 Department of Biotechnology, Sinhgad College of Engineering, Pune, Maharashtra, India
     

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Lactic acid, an organic acid is a product of glucose fermentation in certain cells of organisms. Lactic acid is a crucial metabolite that has gained immense industrial importance. Lactic acid application have a wide expanse including its use to synthesize various fermentation products in food industry, pharmaceutical solutions for drug delivery, preservative in foodstuffs, production of surfactants used in soaps and detergents, tanning leather, synthesis of bio composites and as a mordant in textile industries. Due to its wide applications, it is necessary to study and optimize fermentative synthesis of lactic acid. Lactic acid bacteria were isolated from sources of sauerkraut and cheese water. Sauerkraut is a fermentation product that has a number of lactic acid bacteria thriving on it including, Lactobacillus plantarum, Leuconostoc argentinum, Lactobacillus brevis, Pediococcus pentosaceus. Cheese being a major source of lactic acid bacteria such as Lactobacillus helveticus, Lb. delbrueckii subsp. bulgaricus, Lactococcus lactis, Lb. casei was investigated as a source for the synthesis of lactic acid. Fermentation parameters were optimized and fermentation was carried out for a period of 5 days. Extraction method was followed to separate and purify lactic acid produced. CaCO3 precipitation was carried out to separate lactate from other impurities, followed by acidification with concentrated sulphuric acid. This method showed lesser concentration of lactic acid, so it was further optimized to concentrate the product. In the second method, the concentration of sulphuric acid was lowered (63%) as it outweighed the product and the main impurity water was removed by distillation. CaCO3 precipitation, followed by acidification and distillation is an inexpensive technique in comparison to other techniques. The yield of lactic acid produced was checked by using buttermilk powder as a standard and it was found to be 42.78% and 61% respectively for sauerkraut and cheese mixed cultures.
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  • Y. J. Wee, J. N. Kim, and H. W. Ryu, “Biotechnological production of lactic acid and its recent applications,” Food Technology and Biotechnology, vol. 44, no. 2, pp. 163-172, 2006.

  • G. R. Antonio, G. Vaccari, E. Dosi, A. Trilli, M. Rossi, and D. Matteuzzi, “Enhanced production of L-(+)-lactic acid in chemostat by Lactobacillus casei DSM 20011 using ion-exchange resins and cross-flow filtration in a fully automated pilot plant controlled via NIR,” Biotechnology and Bioengineering, vol. 67, no. 2, pp. 147-156, 2000.

  • X. Han, and J. Pan, “A model for simultaneous crystallisation and biodegradation of biodegradable polymers,” Biomaterials, vol. 30, no. 3, pp. 423-430, 2009.

  • R. P. John, G. S. Anisha, K. M. Nampoothiri, and A. Pandey, “Direct lactic acid fermentation: Focus on simultaneous saccharification and lactic acid production,” Biotechnology Advances, vol. 27, no. 2, pp. 145-152, 2009.

  • M. G. Fortinaa, G. Riccia, A. Acquatib, G. Zeppab, A. Gandinib, and P. L. Manachinia, “Genetic characterization of some lactic acid bacteria occurring in an artisanal Protected Denomination Origin (PDO) Italian cheese, the Toma piemontese,” Food Microbiology, vol. 20, pp. 397-404, 2003.

  • V. H. Shiphrah, S. Sahu, A. R. Thakur, and S. R. Chaudhuri, “Screening of bacteria for lactic acid prodution from whey water,” American Journal of Biochemistry and Biotechnology, vol. 9, no. 2, pp. 118-123, 2013.

  • Q. Z. Li, X. L. Jiang, X. J. Feng, J. M. Wang, C. Sun, H. B. Zhang, M. Xian, and H. Z. Liu, “Recovery processes of organic acids from fermentation broths in the biomass-based industry,” J. Microbiol. Biotechnol., vol. 26, no. 1, pp. 1-8, 2016.


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  • Fermentative Synthesis of Lactic Acid

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Authors

Shweta Kashid
Department of Biotechnology, Sinhgad College of Engineering, Pune, Maharashtra, India
Tejashree Dhamale
Department of Biotechnology, Sinhgad College of Engineering, Pune, Maharashtra, India
Srushti Deshpande
Department of Biotechnology, Sinhgad College of Engineering, Pune, Maharashtra, India

Abstract


Lactic acid, an organic acid is a product of glucose fermentation in certain cells of organisms. Lactic acid is a crucial metabolite that has gained immense industrial importance. Lactic acid application have a wide expanse including its use to synthesize various fermentation products in food industry, pharmaceutical solutions for drug delivery, preservative in foodstuffs, production of surfactants used in soaps and detergents, tanning leather, synthesis of bio composites and as a mordant in textile industries. Due to its wide applications, it is necessary to study and optimize fermentative synthesis of lactic acid. Lactic acid bacteria were isolated from sources of sauerkraut and cheese water. Sauerkraut is a fermentation product that has a number of lactic acid bacteria thriving on it including, Lactobacillus plantarum, Leuconostoc argentinum, Lactobacillus brevis, Pediococcus pentosaceus. Cheese being a major source of lactic acid bacteria such as Lactobacillus helveticus, Lb. delbrueckii subsp. bulgaricus, Lactococcus lactis, Lb. casei was investigated as a source for the synthesis of lactic acid. Fermentation parameters were optimized and fermentation was carried out for a period of 5 days. Extraction method was followed to separate and purify lactic acid produced. CaCO3 precipitation was carried out to separate lactate from other impurities, followed by acidification with concentrated sulphuric acid. This method showed lesser concentration of lactic acid, so it was further optimized to concentrate the product. In the second method, the concentration of sulphuric acid was lowered (63%) as it outweighed the product and the main impurity water was removed by distillation. CaCO3 precipitation, followed by acidification and distillation is an inexpensive technique in comparison to other techniques. The yield of lactic acid produced was checked by using buttermilk powder as a standard and it was found to be 42.78% and 61% respectively for sauerkraut and cheese mixed cultures.

References