Asian Journal of Bio Science

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Role of Cytological Markers for Evaluation of Genetic Integrity of in Vitro Regenerated Plants


Affiliations
1 Department of Dairy and Food Microbiology, College of Dairy and Food Science Technology, Maharana Pratap University of Agriculture and Technology, Udaipur (Rajasthan), India
     

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Micropropagation can be rewarding only if complete genetic fidelity of micropropagules is maintained. Genetic fidelity is the maintenance of genetic constitution of a particular clone throughout its growth span (Chatterjee and Prakash, 1996). Periodic monitoring of the degree of genetic stability of in vitro conserved plants is of utmost importance for commercial utilization of true-to-type plants of the desired genotype (Mohanty et al., 2011). The assessment of the genetic integrity of in vitro grown regenerants in regular intervals can significantly reduce or eliminate the chance of occurrence of somaclonal variation (Larkin and Scowcroft, 1981) at early or late phase of culture.

Keywords

Cytological Marker, Somaclonal Variation, Genetic Fidelity, Tissue Culture.
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  • Role of Cytological Markers for Evaluation of Genetic Integrity of in Vitro Regenerated Plants

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Authors

Manish Chittora
Department of Dairy and Food Microbiology, College of Dairy and Food Science Technology, Maharana Pratap University of Agriculture and Technology, Udaipur (Rajasthan), India
Aradhana Sukhwal
Department of Dairy and Food Microbiology, College of Dairy and Food Science Technology, Maharana Pratap University of Agriculture and Technology, Udaipur (Rajasthan), India

Abstract


Micropropagation can be rewarding only if complete genetic fidelity of micropropagules is maintained. Genetic fidelity is the maintenance of genetic constitution of a particular clone throughout its growth span (Chatterjee and Prakash, 1996). Periodic monitoring of the degree of genetic stability of in vitro conserved plants is of utmost importance for commercial utilization of true-to-type plants of the desired genotype (Mohanty et al., 2011). The assessment of the genetic integrity of in vitro grown regenerants in regular intervals can significantly reduce or eliminate the chance of occurrence of somaclonal variation (Larkin and Scowcroft, 1981) at early or late phase of culture.

Keywords


Cytological Marker, Somaclonal Variation, Genetic Fidelity, Tissue Culture.

References