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Isolation and PGPR Characterization of Cadmium Resistant Strains RCP 1 in Cadmium Contaminated Soil


Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
2 Department of Microbiology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur (U.P.), India
     

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Resistance and tolerance are arbitrarily defined terms frequently interchangeable and after based on weather a given isolate can grow in the presence of selected heavy metal concentration in laboratory media. Total bacterial strains were isolated and screened on the basis of their heavy metal tolerance ability for cadmium and their PGPR activity. It was estimated that they have good tolerance power for cadmium, the heavy metals and are plant growth promoting rhizobacteria. If we study morphological aspects we can say that Gram negative rods are more efficient in tolerating heavy metals. By performing different PGPR test it was proved that the isolated strains have PGPR activity can be used as biopesticides and biofertilizers. At pH 7 and 280C temperature strain RCP 1 was resistant to heavy metal but at pH 9, the growth was inhibited in presence of heavy metal. There were high growth rate of strain RCP 1 at 280C. Strain RCP 1 grew at neutral and alkaline pH also, but in presence of heavy metal growth was increased. In presence of heavy metal, strain RCP 1 increased the ischolar_main and shoot length as well as fresh and dry weight also. So, the microbes isolated from RCP 1 that must have good tolerance power and developed resistance mechanism for their survival and thus, can be helpful in bioremediation.


Keywords

Rhizobacteria, Tolerance, Resistance, PGPR, Cadmium.
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  • Isolation and PGPR Characterization of Cadmium Resistant Strains RCP 1 in Cadmium Contaminated Soil

Abstract Views: 316  |  PDF Views: 2

Authors

Anjali Tiwari
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
O. P. Verma
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Manishi Tripathi
Department of Microbiology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur (U.P.), India

Abstract


Resistance and tolerance are arbitrarily defined terms frequently interchangeable and after based on weather a given isolate can grow in the presence of selected heavy metal concentration in laboratory media. Total bacterial strains were isolated and screened on the basis of their heavy metal tolerance ability for cadmium and their PGPR activity. It was estimated that they have good tolerance power for cadmium, the heavy metals and are plant growth promoting rhizobacteria. If we study morphological aspects we can say that Gram negative rods are more efficient in tolerating heavy metals. By performing different PGPR test it was proved that the isolated strains have PGPR activity can be used as biopesticides and biofertilizers. At pH 7 and 280C temperature strain RCP 1 was resistant to heavy metal but at pH 9, the growth was inhibited in presence of heavy metal. There were high growth rate of strain RCP 1 at 280C. Strain RCP 1 grew at neutral and alkaline pH also, but in presence of heavy metal growth was increased. In presence of heavy metal, strain RCP 1 increased the ischolar_main and shoot length as well as fresh and dry weight also. So, the microbes isolated from RCP 1 that must have good tolerance power and developed resistance mechanism for their survival and thus, can be helpful in bioremediation.


Keywords


Rhizobacteria, Tolerance, Resistance, PGPR, Cadmium.