Asian Journal of Bio Science

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Combined Mutagenic Improvement of Bacillus licheniformis SK7 for Cost-Effective Protease Production


Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
2 Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak (Haryana), India
     

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The strain improvement of developed Bacillus licheniformis SK7 was achieved with the combination of physical and chemical agents i.e., UV + NTG + EMS by gradually mutation and positive selection. In case of UV light 99 per cent killing for successful selection of mutants was 6 min and 10 cm distance from source, while in case of EMS and NTG the concentration found effective was 200 μM, 30 μM, respective for 15 min. The mutant Bacillus licheniformis SK7 SN 43 was successfully developed and found stable having higher production of protease (662 U/ml) under optimized medium and physical conditions of fermentation than the wild type Bacillus licheniformis SK7 (472 U/ml) under the same conditions of fermentation.


Keywords

Mutagenesis, NTG, UV Light, Strain Improvement, Protease.
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  • Combined Mutagenic Improvement of Bacillus licheniformis SK7 for Cost-Effective Protease Production

Abstract Views: 310  |  PDF Views: 2

Authors

Sanjay Kumar Yadav
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Poonam Singh
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Kashyap Kumar Dubey
Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak (Haryana), India
Bhanu P. Singh
Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak (Haryana), India

Abstract


The strain improvement of developed Bacillus licheniformis SK7 was achieved with the combination of physical and chemical agents i.e., UV + NTG + EMS by gradually mutation and positive selection. In case of UV light 99 per cent killing for successful selection of mutants was 6 min and 10 cm distance from source, while in case of EMS and NTG the concentration found effective was 200 μM, 30 μM, respective for 15 min. The mutant Bacillus licheniformis SK7 SN 43 was successfully developed and found stable having higher production of protease (662 U/ml) under optimized medium and physical conditions of fermentation than the wild type Bacillus licheniformis SK7 (472 U/ml) under the same conditions of fermentation.


Keywords


Mutagenesis, NTG, UV Light, Strain Improvement, Protease.