Callus Induction and Organogenesis in Chandrasoor (Lepidium sativum L.)

The experiment was carried out in the Tissue culture laboratory, College of Agriculture, Indore J.N.K.V.V. (M.P.), during the session 2008-2009. Seven media were tried under the study. Of them, five were of Murashige and Skoog's basal medium with various concentrations and combinations of growth hormones and the other two media were Gamborg B₅ and White's media. The three explants used in this investigation were stem disc, leaf base and leaf blade (middle). With regard to callusing percentage and callus growth, some modifications of MS medium gave high callusing efficiency, as compared to other MS combinations. Medium M₄ was the best, which contained MS + 0.2mg/l BAP +1mg/l Kinetin + 2mg/l IAA. The callusing was less in medium M₁ (MS + 0.5mg/l 2,4-D) followed by Medium M₃ (MS + 2mg/l 2,4-D + 1 mg/l Kinetin + 0.5 mg/ l BAP). The decreasing order of effectiveness of different media tried was M₄> M₅> M₃> B₅ > White's > M₂ > M₁. Among the explants used, stem disc was found to be the best explant closely followed by the leaf base. Thus, the present investigation suggested the use of stem disc and leaf base for callusing. Regarding shoot regeneration M₄ medium containing 1/2 MS salts + 0.2mg/l BAP + 0.05 mg/l Kinetin + 1mg/l IAA was the best followed by M₅ with MS salts + 1mg/l Kinetin + 2mg/l NAA + 0.5mg/l GA₃. For ischolar_main regeneration, M₄ medium containing 1/2 MS salts + 0.2mg/ l BAP + 0.05 mg/l Kinetin + 1mg/l IAA was found to be the best. Darkness was found to be favourable for ischolar_main regeneration.