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Cytogenetic Studies of Psoralea corylifolia Linn. due to Exposure of Physical Mutagens (Gamma Radiations)


Affiliations
1 Department of Botany, Shivaji Science College, Nagpur (M.S.), India
2 Department of Biotechnology, Shivaji Science College, Nagpur (M.S.), India
     

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Mutations are the important tools to produce many cultivars with improved economic value and study of genetics and plant development phenomena. Various mutagenic agents are used to induce favourable mutations at high frequency that include ionizing radiation and chemical mutagens. In the present study, the effect of physical mutagens i.e. gamma radiations have been studied on the cytogenetic of Psoralea corylifolia Linn. seeds. Different dosages of gamma rays were used to irradiate ranging from 5KR to 80KR. The frequency of dividing cells were increased at lower exposures while found decreased at higher exposures in M1 generations of Psoralea corylifolia. The mitotic index increased upto 20KR while it decreased at high exposures. Various chromosomal aberrations were recorded in mitosis like laggards, bridges, clumping of chromosomes and precocious movement of chromosomes. The frequency of abnormalities increased with increase in dose of gamma rays.

Keywords

Psoralea corylifolia, Physical Mutagens, Chromosomal Abnormalities.
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  • Cytogenetic Studies of Psoralea corylifolia Linn. due to Exposure of Physical Mutagens (Gamma Radiations)

Abstract Views: 291  |  PDF Views: 0

Authors

R. N. Deshmukh
Department of Botany, Shivaji Science College, Nagpur (M.S.), India
N. A. Tambe
Department of Biotechnology, Shivaji Science College, Nagpur (M.S.), India

Abstract


Mutations are the important tools to produce many cultivars with improved economic value and study of genetics and plant development phenomena. Various mutagenic agents are used to induce favourable mutations at high frequency that include ionizing radiation and chemical mutagens. In the present study, the effect of physical mutagens i.e. gamma radiations have been studied on the cytogenetic of Psoralea corylifolia Linn. seeds. Different dosages of gamma rays were used to irradiate ranging from 5KR to 80KR. The frequency of dividing cells were increased at lower exposures while found decreased at higher exposures in M1 generations of Psoralea corylifolia. The mitotic index increased upto 20KR while it decreased at high exposures. Various chromosomal aberrations were recorded in mitosis like laggards, bridges, clumping of chromosomes and precocious movement of chromosomes. The frequency of abnormalities increased with increase in dose of gamma rays.

Keywords


Psoralea corylifolia, Physical Mutagens, Chromosomal Abnormalities.