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Screening of Anti-Bacterial Activity of C-Phycocyanin and its Minimum Inhibitory Concentration (MIC) Determination by Agar Dilution Method


Affiliations
1 Department of Microbiology, S.R.M. Medical College Hospital and Research Institute, S.R.M. University, Kattankulathur, Kancheepuram (T.N.), India
     

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C-phycocyanin was extracted from laboratory grown Spirulina platensis, and the identity was confirmed using standard Cphycocyanin, by HPTLC and SDS-PAGE. It was then partially purified and screened for antibacterial activity. The results were compared with standard C-phycocyanin (Sigma-Aldrich) and standard antibiotics. There were 6 bacterial isolates (Clinical isolates) assayed for antimicrobial activity. The study proved that the C-phycocyanin possessed antibacterial activity which was more potent than standard C-phycocyanin.

Keywords

C-Phycocyanin, Antimicrobial Activity, Partial Purification, Screening, Minimum Inhibitory Concentrations.
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  • Screening of Anti-Bacterial Activity of C-Phycocyanin and its Minimum Inhibitory Concentration (MIC) Determination by Agar Dilution Method

Abstract Views: 259  |  PDF Views: 0

Authors

T. Murugan
Department of Microbiology, S.R.M. Medical College Hospital and Research Institute, S.R.M. University, Kattankulathur, Kancheepuram (T.N.), India
Radhamadhavan
Department of Microbiology, S.R.M. Medical College Hospital and Research Institute, S.R.M. University, Kattankulathur, Kancheepuram (T.N.), India

Abstract


C-phycocyanin was extracted from laboratory grown Spirulina platensis, and the identity was confirmed using standard Cphycocyanin, by HPTLC and SDS-PAGE. It was then partially purified and screened for antibacterial activity. The results were compared with standard C-phycocyanin (Sigma-Aldrich) and standard antibiotics. There were 6 bacterial isolates (Clinical isolates) assayed for antimicrobial activity. The study proved that the C-phycocyanin possessed antibacterial activity which was more potent than standard C-phycocyanin.

Keywords


C-Phycocyanin, Antimicrobial Activity, Partial Purification, Screening, Minimum Inhibitory Concentrations.