Asian Journal of Bio Science

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Study the Genetic Variations among the Azotobacter chroococcum Isolates using Randomly Amplified Polymorphic DNA (RAPD) Marker


Affiliations
1 Institute of BioScience, Ganpat University, Mehsana (Gujarat), India
2 Central Salt & Marine Chemical Research Institute, Bhavnagar (Gujarat), India
3 Department of Medicinal Crop, Anand Agricultural University, Anand (Gujarat), India
4 Nutan Science College, Visnagar (GUJARAT), India
     

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The geographical area of Karnataka is classified into ten agroclimatic zones. The first species of the genus Azotobacter named Azotobacter chroococcum, was isolated from the soil.The investigation was carried out to study the genetic variation of Azotobacter chrococcum occurs in the soils of the ten different agroclimatic zones of Karnataka. These isolates were characterized by using RAPD markers. A total of 103 bands were scored out of which 87 bands were found to be polymorphic (84.97%). Statistical analysis of RAPD data enabled the classification of 10 A. chroococcum isolates into three major groups. The RAPD banding pattern of these isolates could easily distinguish the isolates of different zones. In this the cluster analysis based on 103 RAPD bands revealed that the ten A. chroococcum isolates examined, clustered at a linkage distance of about 55 units on the dendrogram.

Keywords

Azotobacter chrococcum, Waksman No. 77 N-Free Medium, Rapd, Opb-12, Hind3 Marker, OPD-05.
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  • Study the Genetic Variations among the Azotobacter chroococcum Isolates using Randomly Amplified Polymorphic DNA (RAPD) Marker

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Authors

K. Mehta
Institute of BioScience, Ganpat University, Mehsana (Gujarat), India
Y. B. Das
Central Salt & Marine Chemical Research Institute, Bhavnagar (Gujarat), India
N. V. Upadhayay
Department of Medicinal Crop, Anand Agricultural University, Anand (Gujarat), India
S. B. Doshi
Nutan Science College, Visnagar (GUJARAT), India

Abstract


The geographical area of Karnataka is classified into ten agroclimatic zones. The first species of the genus Azotobacter named Azotobacter chroococcum, was isolated from the soil.The investigation was carried out to study the genetic variation of Azotobacter chrococcum occurs in the soils of the ten different agroclimatic zones of Karnataka. These isolates were characterized by using RAPD markers. A total of 103 bands were scored out of which 87 bands were found to be polymorphic (84.97%). Statistical analysis of RAPD data enabled the classification of 10 A. chroococcum isolates into three major groups. The RAPD banding pattern of these isolates could easily distinguish the isolates of different zones. In this the cluster analysis based on 103 RAPD bands revealed that the ten A. chroococcum isolates examined, clustered at a linkage distance of about 55 units on the dendrogram.

Keywords


Azotobacter chrococcum, Waksman No. 77 N-Free Medium, Rapd, Opb-12, Hind3 Marker, OPD-05.