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Somatic Embryogenesis in Kalazira (Bunium persicum Bioss.)
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In vitro multiplication of Bunium persicum via somatic embryogenesis is reported. The callus was derived from petiole explants on Murashige and Skoog medium containing 2mgl-1 2,4-D and 2mgl-1 Kn differentiated globular embryos upon subculture to MS medium supplemented with different concentration of auxins. The globular embryos continued to multiply on a medium supplemented with 2,4-D but failed to mature. Further differentiation of globular embryos occurred when embryogenic callus was sub cultured to a medium lacking 2,4-D. However, the globular embryos formed on NAA and IBA supplemented medium differentiated into mature embryos on the same medium. The mature embryos were germinated on a medium supplemented with 0.2 mgl-1 Kn and 0.01 mgl-1 IBA at a temperature of 10-15°C. The plantlets produced a small tuber on their ischolar_mains which sprouted after 8 weeks.
Keywords
Bunium persicum, Callus Formation, Somatic Embryogenesis.
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