Asian Journal of Bio Science

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Role of Detoxifying Enzymes in Host Plant Resistance to Cotton Mealybugs (Phenococcus solenopsis Tinsley)


Affiliations
1 Department of Agricultural Entomology, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
2 Department of Agricultural Entomology, K.K. Wagh College of Agriculture, Nashik (M.S.), India
     

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A laboratory investigation was conducted to find out role of detoxifying enzymes in defense mechanism of cotton cultivars against mealybugs in Insect Biotechnology Laboratory of Department of Agricultural Entomology,Dr. PDKV, Akola during 2008-09. Quantitative and qualitative studies undertaken for estimation of detoxifying enzymes like Glutathione S-transferases and esterases. GST activity was found higher in resistant variety, PKV Hy-2, followed by AKH-3614-10 (Hirsutum pigmented). The highest esterase activity was found to be present in Bunny-Bt (187.14 nM mg protein-1 min-1) than other cotton cultivars. PKV Hy-2 showed very lowest esterase activity (76.97 nM mg protein-1 min-1). Glutathione-s-transferase and esterase bands were not detected in the susceptible CAHH-231 (Pigmented hybrid) variety. Three GST isozymes were observed in AKA-8, whereas, CAHH-231 (Pigmented hybrid) did not show any isozyme. The study will be helpful in understanding the biochemical basis of mealy bug resistance in cotton. The outcome of the present investigation will act as stepping stone to develop mealy bug resistant cotton variety.

Keywords

Cotton, Detoxifying Enzymes, Esterases, GST, Host Plant Resistance, Phenococcus solenopsis.
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  • Role of Detoxifying Enzymes in Host Plant Resistance to Cotton Mealybugs (Phenococcus solenopsis Tinsley)

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Authors

Suchita R. Ghule
Department of Agricultural Entomology, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
U. P. Barkhade
Department of Agricultural Entomology, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
M. P. Moharil
Department of Agricultural Entomology, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), India
T. B. Ugale
Department of Agricultural Entomology, K.K. Wagh College of Agriculture, Nashik (M.S.), India

Abstract


A laboratory investigation was conducted to find out role of detoxifying enzymes in defense mechanism of cotton cultivars against mealybugs in Insect Biotechnology Laboratory of Department of Agricultural Entomology,Dr. PDKV, Akola during 2008-09. Quantitative and qualitative studies undertaken for estimation of detoxifying enzymes like Glutathione S-transferases and esterases. GST activity was found higher in resistant variety, PKV Hy-2, followed by AKH-3614-10 (Hirsutum pigmented). The highest esterase activity was found to be present in Bunny-Bt (187.14 nM mg protein-1 min-1) than other cotton cultivars. PKV Hy-2 showed very lowest esterase activity (76.97 nM mg protein-1 min-1). Glutathione-s-transferase and esterase bands were not detected in the susceptible CAHH-231 (Pigmented hybrid) variety. Three GST isozymes were observed in AKA-8, whereas, CAHH-231 (Pigmented hybrid) did not show any isozyme. The study will be helpful in understanding the biochemical basis of mealy bug resistance in cotton. The outcome of the present investigation will act as stepping stone to develop mealy bug resistant cotton variety.

Keywords


Cotton, Detoxifying Enzymes, Esterases, GST, Host Plant Resistance, Phenococcus solenopsis.