Morphogenetic Response on Leaf Explants of Cucumus melo cv. BATHASA

Impact of various growth regulators on tissue and isolated plant cells was studied by several workers and these substances were known to play an important role in cell metabolism, cell membrane synthesis (Cocking, 1978) besides growth and differentiation (Butenko, 1968; Stress, 1969), of these growth substances auxins have proved to be an essential supplement for establishing successful culture of plant tissues (Gautheret, 1959; Rao and Swamy, 1972). Auxins were found to exert their hormonal action through their effects on the nucleic acids and protein metabolism. It was also found that auxins exert their effects, directly or indirectly on ‘S’ phase of DNA synthesis (Peaud-Lenoel, 1977). Cytokinins were found to influence cell growth by promoting nucleic acid metabolism and synthesis of specific proteins required for cell division. It was further reported that cytokinins bind to a specific receptor site in the cell to bring about organogenesis (Yamada et al., 1972). Physical environment viz., state of medium, light temperature humidity etc., besides source and size of explants are known to play an important role in the in vitro organogenesis.