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Transformation of Elite Rice Cultivars ASD16 And IR64 with cry2Ac Gene for Resistance to Rice Lepidopteran Pests
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With the view to imparting resistance to major lepidopteran pests of rice, attempts were made to transform elite indica genotype ASD16 and IR64 with cry2Ac gene. Agrobacterium and particle bombardment-mediated transformation were carried out using the vector pS2AcP2 harbouring cry2Ac gene, the selectable marker gene, hph and the scorable marker gene, gusA. In Agrobacterium-mediated transformation experiment with mature seed derived calli, two lines in ASD16 were regenerated with a regeneration frequency of 1.1 per cent. In particle bombardment-mediated transformation experiments with immature embryos, 15 lines in IR64 were regenerated with a regeneration frequency of six per cent. Presence of transgenes in these lines could not be demonstrated through PCR.
Keywords
Rice, Agrobacterium tumifaciens, Bacillus thuringiensis, Stemborer, Leaffolder, Cry2ac Gene, Biolistic Gun.
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