In Vitro Cytotoxic Effect of Leaves and Stem Bark of Azadirachta indica on Human Colon, Liver, Neurablastoma and Prostate Cancer Cell Lines

Cancer still remains the major cause of mortality and morbidity all over the world. In the search of potential anticancer agents from the medicinal plants, the present research work was carried out to examine the anticancer properties of Azadirachta indica leaves and stem bark against human cancer cell lines via., ethanolic, hydro-ethanolic and aqueous extracts using sulphorhodamine B (SRB) dye. All the three extracts from leaf part and stem-bark showed in vitro cytotoxicity against all the human cancer cell lines at 100 μg/ml. At lower doses (10 and 30 μg/ml) aqueous extract from leaf part was found to be more active than ethanolic and hydro-ethanolic extracts in dose dependent manner. Results showed the potent anticancer effect of Azadirachta indica (leaves and stem bark) on human cancer cell lines of colon, liver, neurablastoma and prostate origin and the plant can be explored for probable anticancer lead molecules for the drug development.