Asian Journal of Bio Science

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Enhancement of Growth and Biological Activity of Selected Actinomycetes Strains of Melissa officinalis and Heracleum candicans on Different Media


Affiliations
1 Department of Microbiology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
2 Department of Mycology and Plant Pathology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
3 Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
     

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Actinomycetes strains isolated from rhizosphere of two important medicinal plants Melissa officinalis and Heracleum candicans were evaluated for production of biological and proteolytic activities by selecting different media. In present investigations two strains Act-M-3 and Act-M-5 of Melissa officinalis produced maximum growth on Glucose ammonium salts (GAS) and Glycerol peptone beef (GPB) broths while strain Act-M-8 preferred GPB. Antibacterial and antifungal activities were registered more on GAS, GPB in addition to Nutrient broth (NB) against Bacillus subtilis, Alternaria and Pythium sp. by two strains Act-M-3 and Act-M-8 in comparison to Act-M-5. Proteolytic production was registered highest in Starch Broth (SB) by Act-M-3 than other two strains. However, Act-H-2 strain isolated from H. candicans recorded maximum growth on GPB while Act-H-5 and Act-H-6 obtained higher production on SB. GAS broth supported greater antibacterial activity by Act-H-2 and Act-H-6 strain towards E. coli and B. subtilis but less or weak effect was obtained by Act-H-5 strain in all the media tested. Antifungal effect against Pythium and Phytopthora sp. was found superior in GPB, GP, GAS and SB. Though proteolytic activity produced by Act-H-2 and Act-H-6 was more on SB, NB and GAS media. Strain Act-H-5 on other hand could not show any proteolytic production on GAS, GPB and SB.

Keywords

Microflora, Nutritional Selection, Secondary Metabolites.
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  • Enhancement of Growth and Biological Activity of Selected Actinomycetes Strains of Melissa officinalis and Heracleum candicans on Different Media

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Authors

Mohinder Kaur
Department of Microbiology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
Sunita Chandel
Department of Mycology and Plant Pathology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
Baldev Kumar
Department of Microbiology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India
Chhaya Sharma
Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.), India

Abstract


Actinomycetes strains isolated from rhizosphere of two important medicinal plants Melissa officinalis and Heracleum candicans were evaluated for production of biological and proteolytic activities by selecting different media. In present investigations two strains Act-M-3 and Act-M-5 of Melissa officinalis produced maximum growth on Glucose ammonium salts (GAS) and Glycerol peptone beef (GPB) broths while strain Act-M-8 preferred GPB. Antibacterial and antifungal activities were registered more on GAS, GPB in addition to Nutrient broth (NB) against Bacillus subtilis, Alternaria and Pythium sp. by two strains Act-M-3 and Act-M-8 in comparison to Act-M-5. Proteolytic production was registered highest in Starch Broth (SB) by Act-M-3 than other two strains. However, Act-H-2 strain isolated from H. candicans recorded maximum growth on GPB while Act-H-5 and Act-H-6 obtained higher production on SB. GAS broth supported greater antibacterial activity by Act-H-2 and Act-H-6 strain towards E. coli and B. subtilis but less or weak effect was obtained by Act-H-5 strain in all the media tested. Antifungal effect against Pythium and Phytopthora sp. was found superior in GPB, GP, GAS and SB. Though proteolytic activity produced by Act-H-2 and Act-H-6 was more on SB, NB and GAS media. Strain Act-H-5 on other hand could not show any proteolytic production on GAS, GPB and SB.

Keywords


Microflora, Nutritional Selection, Secondary Metabolites.