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Development of Triploid Plantlets from Immature Endosperm of Garcinia gummi-gutta var. gummigutta (L) Rob
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The inoculated immature endosperm bits of 1cm size onto ½ MS supplemented with 3.0 mgl-1 each of BA and Kinetin induced adventitious buds and subsequent proliferation of multiple shoots. These triploid plantlets were ischolar_mained, hardened and planted out. Development of regeneration protocol from triploid parenchymatous tissue of endosperm not only overcome the dearth of demands for true-to-type propagules but also surpassed the gender dimorphism. Evolving seedlessness through in vitro endosperm culture may also be helpful to avoid the processing difficulty and to support the establishment of domestic HCA extraction unit for Women empowerment.
Keywords
Garcinia gummi-gutta Var. Gummigutta, Polygamodioecious, Kodampuli, Immature Seeds, Endosperm Culture, Triploid, Seedlessness, Life Style Diseases, Shoot Bud Differentiation, Regeneration, Direct Organogenesis.
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