Isolation of Antagonistic Lactobacillus from Cow Dung and its Nutritional Improvement as Probiotic Strain

Different strain of Lactobacilli spp. were isolated from fresh cow dung and designated as A1 to A10. Selected Lactobacillus isolates were then inoculated with different nutritional sources like MRS broth, Packed milk, Sugarcane juice, Mousammi juice and MRS broth + Sugarcane juice and MRS + Mousammi juice. Their antagonistic effect was tested against three selected pathogenic bacteria namely S. aureus, E. coli and Pseudomonas spp. The result clearly showed that some isolates when inoculated with MRS broth + sugarcane juice inhibited the growth of pathogenic strains in varying degree. Isolate A-6 and A-10 were found to be more effective in reducing growth of all three pathogenic species from 10 to 30 mm. Other isolates (A1 and A2) from the same source, were effective against E. coli and Pseudomonas spp. with a zone of inhibition of 15 to 10, respectively but no zone of inhibition was seen against S.aureus.