Asian Journal of Bio Science

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Pearl Millet Characterization by Using ISSR Marker


Affiliations
1 MGM College of Agricultural Biotechnology, Aurangabad (M.S.), India
2 College of Agriculture Biotechnology, Latur (M.S.), India
3 Banana Research Station, Nanded (M.S.), India
4 Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), India
     

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The present study was carried out with emphasis on ISSR profiling and genetic relationship studies on pearl millet using ISSR marker. The experimental material consisted of 15 genotypes of pearl millet. The seeds of 15 genotypes of pearl millet were grown in petriplates and the young leaves from them were used for DNA extraction. Extraction of total genomic DNA was carried out following the methods suggested by Saghai-Maroof et al. (1984) with minor modification. The selected primers generated 123 ISSR bands and the size of the amplification products ranged from 150-35000 bp. The number of bands generated per primer ranged from 09 (CA)6RG to 19 (AC)8YA with a mean of 13.6 bands per primer. Out of the bands generated 80 were polymorphic and 43 were monomorphic. The genotype GHB 235 showed maximum similarity (0.92) whereas, minimum was for ICMV 155 (0.55) among the genotypes studied.

Keywords

Pearl Millet, Genetic Diversity, ISSR.
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  • Pearl Millet Characterization by Using ISSR Marker

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Authors

V. D. Deshmukh
MGM College of Agricultural Biotechnology, Aurangabad (M.S.), India
H. B. Patil
College of Agriculture Biotechnology, Latur (M.S.), India
J. D. Deshmukh
Banana Research Station, Nanded (M.S.), India
I. A. Madrap
Department of Botany, Marathwada Agricultural University, Parbhani (M.S.), India

Abstract


The present study was carried out with emphasis on ISSR profiling and genetic relationship studies on pearl millet using ISSR marker. The experimental material consisted of 15 genotypes of pearl millet. The seeds of 15 genotypes of pearl millet were grown in petriplates and the young leaves from them were used for DNA extraction. Extraction of total genomic DNA was carried out following the methods suggested by Saghai-Maroof et al. (1984) with minor modification. The selected primers generated 123 ISSR bands and the size of the amplification products ranged from 150-35000 bp. The number of bands generated per primer ranged from 09 (CA)6RG to 19 (AC)8YA with a mean of 13.6 bands per primer. Out of the bands generated 80 were polymorphic and 43 were monomorphic. The genotype GHB 235 showed maximum similarity (0.92) whereas, minimum was for ICMV 155 (0.55) among the genotypes studied.

Keywords


Pearl Millet, Genetic Diversity, ISSR.