Optimization and Comparative Analysis of Meat Infusion in Muller Medium for The Production of Effective Tetanus Toxin

Optimizations of fermentation medium for Clostridium tetani (MTCC 449) in the effective production of tetanus toxin were studied. Modified Muller Media with different concentration of Meat infusion has been used for the production of tetanus toxin . In this present work, the comparative study was carried out to determine the efficacy of tetanus toxin with the commercially prepared meat infusion broth compared and the meat infusion powder supplemented in Muller medium. The observed results showed the higher titer value of toxin in Muller media supplemented with meat infusion with higher concentration. The OD of the cell growth and pH changes due to buffering capacity were also studied. The better growth of the C.tetani was observed in the MM medium which contain higher rate of meat infusion broth. The level of the tetanus toxin was measured by Lf test. Minimal lethal dose was performed to check the presence of tetanus toxin.