Asian Journal of Bio Science

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Production of Citric Acid by Yarrowia lipolytica Using Biodiesel Derived Glycerol


Affiliations
1 Department of Civil Engineering, National University of Ireland, Galway, Ireland
2 Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
     

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Crude glycerol, a by product of Jatropha biodiesel was used as carbon source for production of citric acid by Yarrowia lipolytica MTCC 35. Concentrations of crude glycerol ranging from 1 to 4 per cent were taken in buffered and unbuffered broth for the optimized production of citric acid by Yarrowia lipolytica MTCC 35. The amount of citric acid produced in buffered broth (11.2 gl-1) was doubled when compared to unbufferd broth (5.2 gl-1). Quantitative estimation through HPLC also confirmed the same.

Keywords

Citric Acid, Yarrowia lipolytica, Crude Glycerol-Biodiesel by Product.
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  • Production of Citric Acid by Yarrowia lipolytica Using Biodiesel Derived Glycerol

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Authors

M. Iniyakumar
Department of Civil Engineering, National University of Ireland, Galway, Ireland
A. Ramalakshmi
Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
P. Santhanakrishnan
Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India

Abstract


Crude glycerol, a by product of Jatropha biodiesel was used as carbon source for production of citric acid by Yarrowia lipolytica MTCC 35. Concentrations of crude glycerol ranging from 1 to 4 per cent were taken in buffered and unbuffered broth for the optimized production of citric acid by Yarrowia lipolytica MTCC 35. The amount of citric acid produced in buffered broth (11.2 gl-1) was doubled when compared to unbufferd broth (5.2 gl-1). Quantitative estimation through HPLC also confirmed the same.

Keywords


Citric Acid, Yarrowia lipolytica, Crude Glycerol-Biodiesel by Product.