Studies on  in vitro  cytotoxicity of  Ficus hispida  Leaves

Madhulika Bhagat; Jatinder Singh Arora; Ajit Kumar Saxena

Studies on in vitro cytotoxicity of Ficus hispida Leaves

Madhulika Bhagat ¹, Jatinder Singh Arora ², Ajit Kumar Saxena ³

Affiliations
1 Department of Biotechnology, University of Jammu, Jammu (J&K), India
2 Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar (Punjab), India
3 Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu (J&K), India

Ficus hispida Linn. is explored for its in vitro cytotoxicty against oral (KB) and colon (COLO 205) human cancer cell lines. Three extracts (alcoholic, hydro-alcoholic and aqueous) and four fractions (n-hexane, chloroform, n-butanol and aqueous) from the leaves were prepared and were evaluated using sulforhodamine B assay at 10, 30 and 100 μg/ml. The growth inhibition demonstrated by all extracts and fractions were in dose dependent manner. The alcoholic extract was most active followed by aqueous and 50% aqueous-alcoholic extract. Further on the evaluation of fractions, n-butanol fraction was highly significant among the four fractions of alcoholic extract against both oral and colon cancer cell lines.