Callus Induction in Simarouba glauca D.C.

Simarouba glauca is an oil yielding tree with a very high productivity and its rapid multiplication is essential for mitigating the edible/industrial oil demand. For developing in vitro regeneration protocol, optimization of surface sterilization of explant and study of effect of auxins and cytokinins is essential. The explant was exposed to mercuric chloride (0.1 %) and Sodium hypochloride (1 %) for a period of 2-12 minutes. Both the surface sterilizing agents gave near about same results with no contamination even at lowest contact time, however, as the contact time was increased intense browning was observed. The callus induction was observed at all the concentration of 2,4-D and BAP. More callusing was observed with higher concentration of both 2,4 D and BAP.