Optimization of β-Glucosidase Assay and Protein Estimation from Various Parts of Rauvolfia serpentina

Rauvolfia serpentina is gifted with unique alkaloids that have remarkable medicinal properties and is being pronounced as “Wonder drug of India”. Several Ayurvedic preparations containing Rauvolfia plant parts are available in the market. The activity of crude enzyme in Rauvolfia serpentina was highest in mature leaf-1 (100%) followed by very young leaf (94.715%), whereas lowest in ischolar_main (9.829%). It was noticed that very young leaf and young leaf have highest protein 7.4573 and 3.8344 mg/ml, respectively and lowest protein content was found in stem (0.1689 mg/ml) of Rauvolfia serpentina. Young leaf contained highest number of isoforms (3).