Studies on Regeneration Potential of Callus in Chickpea Cv. ICCV-2

For callus induction studies explants i.e., shoot tips hypocotyls and ischolar_mains were excised from six days aseptically grown seedlings. NAA 2 mg/l+BAP 2 mg/l was found best among various combinations used for induction of callus. For differentiation of callus BAP 1 mg/l+NAA 0.5 mg/l treatment combination was found to be the best that resulted in maximum number of shoot lets, for differentiation followed by profuse ischolar_maining to the regenerated shoots. For the completely developed plantlets, the maximum survival was observed with the hardening treatment where in the plants were directly transferred into polybags were kept in mist chamber.