Isolation, Characterization and Evaluation of Growth of Salt Tolerant Mangrove Microbes Grown Under NaCl Stress

An attempt Was made to evaluate the salt tolerance behaviour of Aspergillus sp. and Bacillus sp. isolated on Brain heart infusion agar from muddy water of Bhitarkanika mangrove ecosystem by growing them under different salt concentration in broth medium . It was observed that fungus could tolerate salt stress upto 16% where as bacteria could be able to grow upto 11 %. The Aspergillus sp. showed increase in growth with the increasing concentration of NaCl where as bacteria declined its growth in this condition. It was observed that acidic metabolites was produced by fungus, therefore, its pH was decreased with the increasing level of NaCl . Simultaneously, bacteria did not show any significant changes in pH of filtrate of different saline treated cultures. The protein content in the culture filtrate of fungus increased in higher concentration of salt . However, bacteria did not behave in the same manner rather showed negative effect of salinity on growth. The present attempt made to analyze the salt stress in fungus and bacteria was of primary nature that provided us various clues to work further.