Asian Journal of Bio Science

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Molecular Dissection of Drought Related QTLs in Rice Using Microsatelite Markers


Affiliations
1 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), India
2 Department of Biotechnology, Indira Gandhi Agricultural University, Raipur (C.G.), India
     

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A double haploid mapping population consisting of 75 lines of a cross between the irrigated indica variety IR64 and the upland japonica varietyAzucena (Guiderdoni et al., 1992) was used in the present experiment. The complete set of double haploid lines along with parents was evaluated for molecular mapping. A set of 264 microsatellite markers was used for parental polymorphism screening of which 60 markers were found polymorphic. Thirty-one of these markers were associated with QTLs of different traits and are distributed on eight different rice chromosomes. These markers can be successfully used to transfer some of the QTLs into different genetic backgrounds.

Keywords

QTLs, Rice, Drought.
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  • Molecular Dissection of Drought Related QTLs in Rice Using Microsatelite Markers

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Authors

M. S. Dudhare
Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), India
S. K. Katiyar
Department of Biotechnology, Indira Gandhi Agricultural University, Raipur (C.G.), India

Abstract


A double haploid mapping population consisting of 75 lines of a cross between the irrigated indica variety IR64 and the upland japonica varietyAzucena (Guiderdoni et al., 1992) was used in the present experiment. The complete set of double haploid lines along with parents was evaluated for molecular mapping. A set of 264 microsatellite markers was used for parental polymorphism screening of which 60 markers were found polymorphic. Thirty-one of these markers were associated with QTLs of different traits and are distributed on eight different rice chromosomes. These markers can be successfully used to transfer some of the QTLs into different genetic backgrounds.

Keywords


QTLs, Rice, Drought.