Asian Journal of Bio Science

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Screening of Rice (Oryza sativa L.) Genotypes with Response to Salinity by RAPD Marker


Affiliations
1 Department of Biochemistry and Biotechnology, B.A. College of Agriculture, A.A.U., Anand (Gujarat), India
     

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Four genotypes of rice (Dandi, CSR-1, IR-36 and GR-3), differing in salt tolerance were grown at 3 and 5 EC (dSm-1) salinity to study the effect of salinity at seedling (15 DAG) stage. The RAPD study indicated total 50.97% polymorphism and the maximum polymorphic loci was obtained by OP-D-8. Dandi at 3 EC and 5 EC recorded 100 per cent band sharing whereas the maximum genetic distance was recorded between CSR-1 (control) and GR-3 (5 EC) salinity. The polymorphisms in RAPDs are useful to study gene expression due to salinity in different rice cultivars.


Keywords

RAPD, Rice, Salinity.
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  • Screening of Rice (Oryza sativa L.) Genotypes with Response to Salinity by RAPD Marker

Abstract Views: 254  |  PDF Views: 0

Authors

Varsha Suthar
Department of Biochemistry and Biotechnology, B.A. College of Agriculture, A.A.U., Anand (Gujarat), India
Nilesh J. Patel
Department of Biochemistry and Biotechnology, B.A. College of Agriculture, A.A.U., Anand (Gujarat), India
Harshal E. Patil
Department of Biochemistry and Biotechnology, B.A. College of Agriculture, A.A.U., Anand (Gujarat), India
R. Bhatnagar
Department of Biochemistry and Biotechnology, B.A. College of Agriculture, A.A.U., Anand (Gujarat), India

Abstract


Four genotypes of rice (Dandi, CSR-1, IR-36 and GR-3), differing in salt tolerance were grown at 3 and 5 EC (dSm-1) salinity to study the effect of salinity at seedling (15 DAG) stage. The RAPD study indicated total 50.97% polymorphism and the maximum polymorphic loci was obtained by OP-D-8. Dandi at 3 EC and 5 EC recorded 100 per cent band sharing whereas the maximum genetic distance was recorded between CSR-1 (control) and GR-3 (5 EC) salinity. The polymorphisms in RAPDs are useful to study gene expression due to salinity in different rice cultivars.


Keywords


RAPD, Rice, Salinity.