Protein Electrophoresis for Identification of Hybrids and their Parents in Sorghum bicolor (L) Moench.

The applicability of protein markers was studied for identification of sorghum hybrid and its parents and to test the genetic purity of commercial samples. The experimental materials consisted of four sorghum hybrids Viz. CHS-14, CSH-9, CSH-19R and CSH-15R and their respective parental lines. The separation of protein on the basis of their molecular mass was performed by denaturing proteins in presence of SDS and then subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis. The hybrid CSH-14 and its parents produced distinguishing protein banding pattern by SDS-PAGE analysis of soluble proteins which was found to be useful in identifying hybrid CSH-14 from its parents and also from the other genotypes under study. No polymorphism in protein profile of hybrid CSH-19R and male parent AKR 354 was observed in the present investigation indicating limitation of SDS-PAGE analysis in CSH-19R for identification and determination of genetic purity and further study to confirm the same. However some of protein markers obtained in hybrid CSH-9 and CSH-15R showed the possibility of application of this technique to some extent for genetic purity analysis in these hybrids.