Assessment of Genetic Diversity among Leaf Blight Resistant and Susceptible Cultivars of Barley by RAPD

Molecular diversity was assessed using two leaf blight resistant and two susceptible genotypes in barley. Out of 25 RAPD primers used, 20 primers produced polymorphism. Similarity coefficients ranged from 0.65 (PL-760 and RD- 2508) to 0.78 (PL-760 and DWR-28) among genotypes. The primers OPA-05, OPM-06, and OPB-11 produced 100 per cent polymorphism. Clustering pattern clearly differentiated resistant (DWR-28 and PL-760) and susceptible genotypes (RD-2508 and RD-2653).