In vitro Rooting Studies in Spine Gourd (Momordica dioica Roxb)

Healthy shoots, which have there own ischolar_main system, are able to survive and become complete plantlets. The healthy well developed multiple shoots were individually separated and kept for ischolar_maining on various media composition. 80 per cent ischolar_maining was obtained in AKSG-5 on RM₆ (MS+AdSO₄ 80 mg/l + IBA 1.0 mg/l). The highest ischolar_maining percent in AKSGM-1 (93%) was obtained on RM3 (MS+IBA 1.0 mg/l) followed by AKSG-35 (86.67%) was observed. Similar results reported by Deokar et al. (2003) and they obtained 80% ischolar_mains in spine gourd on MSHP + ADS 80 mg/l + IBA 1.0 mg/l + 0.9% agar agar and Geeta Kulkarni obtained (86.66%) ischolar_maining response to Kartoli on MS+3ppm NAA + 0.8% Agar + 3% sucrose with activated charcoal. In present investigation, in AKSG-5 (4.33) primary ischolar_mains per culture with 4.17 cm ischolar_main length and in AKSG-35 (4.67) primary ischolar_mains per culture with 4.67 cm ischolar_main length in AKSGM-1 (4.67) primary ischolar_mains with 4.67 cm ischolar_main length were recorded on RM₆ (MS+AdSO₄ 80 mg/l + IBA 1.0 mg/l) were recorded.